Neuroprotective effects of phenylethanoid glycosides in an in vitro model of Alzheimer's disease

Abstract

The present study aimed to investigate the neuroprotective effects of phenylethanol glycosides (PhGs) on HO- and β-amyloid peptide (Aβ)-induced injury of PC12 cells as an model of Alzheimer's disease (AD). The optimal induction conditions were established through screening of various incubation times and concentrations. PC12 cells were treated with 0.5 µM Aβ and HO in the presence of PhGs for 24 h and the cell viability was then evaluated by an MTT assay; lactate dehydrogenase (LDH) release and malondialdehyde (MDA) content were also measured. The optimal conditions for establishing the AD model were the treatment of PC12 cells with 0.5 µM Aβ for 48 h, or with 25 µM HO dissolved in DMEM with PBS. PhGs at concentrations of 5, 25 and 50 µg/ml increased the viability and decreased LDH and MDA release by PC12 cells injured with Aβ or HO. In conclusion, the model of Aβ- and HO-induced PC12 cell injury was successfully established. PhGs were shown to have a significant neuroprotective effect against Aβ- or HO-induced cell injury.

Scutellarin‑treated exosomes increase claudin 5, occludin and ZO1 expression in rat brain microvascular endothelial cells

“…Oxidative stress-related injury and apoptosis increase cell membrane permeability, resulting in the release of LDH (21). As such, LDH activity in the cell culture medium is proportional to cell death (22). For normal RBMVECs, 5–200 µmol/l Scu was not observed to cause significant toxicity at 24 h. However, 2.5 mmol/l Hcy was observed to be toxic to normal RBMVECs, and so this concentration was selected for subsequent experiments.…”

Therapeutic potential of phenylethanoid glycosides: A systematic review

“…87 Caleolarioside B, paraboside B, and paraboside II isolated from Paraboea martinii effectively protected PC12 cells from H 2 O 2induced damage by upregulating HO-1. 88 It is believed that β amyloid peptide (Aβ) is a major cause of Alzheimer's disease. 89 Total PhGs extracted from Cistanches herba at concentrations of 5, 25, and 50 μg/ml increased the viability and decreased lactate dehydrogenase and malondialdehyde (MDA) release by PC12 cells injured with Aβ 1-42 .…”

Protective role of phenylethanoid glycosides, Torenoside�B and Savatiside A, in Alzheimer's disease

“…They may also be used as potential drugs for kidney protection and laxative effect treatments (9). Previous studies have revealed that PhGs extracts can be used to treat a variety of coronary artery diseases (7), respiratory infections and pneumonia (10). In a recent report, PhGs were demonstrated to influence the structure and function of neurons and thus, may be used as neuroprotective agents against H 2 O 2 and Aβ 1-42 -induced injury in PC12 cells (11).…”

“…According to previous studies, PhGs are considered to be potential drug candidates for the treatment of AD (7,8,10). However, few reports assess the neuroprotective effect of TB and SA extracted from Monochasma savatieri Franch, in the treatment of AD as well as the signal pathways utilized.…”