Neuroprotective effects of Tiaogeng decoction against H2O2-induced oxidative injury and apoptosis in PC12 cells via Nrf2 and JNK signaling pathways

Gao, Xianwei; Li, Shengnan; Liu, Xiaofei; Cong, Chao; Zhao, Li; Liu, Huicong; Xu, Luwei

doi:10.1016/j.jep.2021.114379

Cited by 10 publications

(6 citation statements)

References 59 publications

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“…Similar trends were also observed with respect to HO-1 expression (Figure F,G right). Our results are consistent with data generated by Gao et al, who found that antioxidant treatment was sufficient to alleviate the oxidative stress-mediated inhibition of the NRF2/HO-1 pathway . Our results thus confirmed the biosafety of PBNPs while demonstrating their ability to effectively protect against cell death via ROS scavenging and the restoration of endogenous antioxidant pathway activity.…”

Section: Resultssupporting

confidence: 92%

Thermosensitive Hydrogel Incorporating Prussian Blue Nanoparticles Promotes Diabetic Wound Healing via ROS Scavenging and Mitochondrial Function Restoration

Zhao

Liu

et al. 2022

ACS Appl. Mater. Interfaces

115

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Diabetic foot ulcer is a serious complication in diabetes patients, imposing a serious physical and economic burden to patients and to the healthcare system as a whole. Oxidative stress is thought to be a key driver of the pathogenesis of such ulcers. However, no antioxidant drugs have received clinical approval to date, underscoring the need for the further development of such medications. Hydrogels can be applied directly to the wound site, wherein they function to prevent infection and maintain local moisture concentrations, in addition to serving as a reservoir for the delivery of a range of therapeutic compounds with the potential to expedite wound healing in a synergistic manner. Herein, we synthesized Prussian blue nanoparticles (PBNPs) capable of efficiently scavenging reactive oxygen species (ROS) owing to their ability to mimic the activity of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD). In the context of in vitro oxidative stress, these PBNPs were able to protect against cytotoxicity, protect mitochondria from oxidative stress-related damage, and restore nuclear factor erythroid 2-related factor 2 (NRF2)/heme oxygenase-1 (HO-1) pathway activity. To expand on these results in an in vivo context, we prepared a thermosensitive poly (d,L-lactide)-poly(ethylene glycol)-poly(d,L-lactide) (PDLLA-PEG-PDLLA) hydrogel (PLEL)-based wound dressing in which PBNPs had been homogenously incorporated, and we then used this dressing as a platform for controlled PBNP release. The resultant PBNPs@PLEL wound dressing was able to improve diabetic wound healing, decrease ROS production, promote angiogenesis, and reduce pro-inflammatory interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) levels within diabetic wounds. Overall, our results suggest that this PBNPs@PLEL platform holds great promise as a treatment for diabetic foot ulcers.

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Section: Resultssupporting

confidence: 92%

Thermosensitive Hydrogel Incorporating Prussian Blue Nanoparticles Promotes Diabetic Wound Healing via ROS Scavenging and Mitochondrial Function Restoration

Zhao

Liu

et al. 2022

ACS Appl. Mater. Interfaces

115

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“…Some studies have shown that the degree of membrane permeability on bEnd.3 cells can indirectly indicate the penetration of BBB since bEnd.3 is a reliable BBB model [34,35]. Neuronal cell uptakes of CFPE-Lip and CFPE-Glu-Lip were tested on PC12 cells, a neuronal cell model, as the degree of membrane permeability on PC12 cells can indirectly indicate the targeting and uptake effects of neuronal cells [36,37]. The results show that the uptake of glucose-modified liposome CFPE-Glu-Lip on bEnd.3 cells was The evaluations were simultaneously performed by qualitative observation using laser confocal microscopy (Figure 7B).…”

Section: Discussionmentioning

confidence: 99%

A Novel Quercetin Encapsulated Glucose Modified Liposome and Its Brain-Target Antioxidative Neuroprotection Effects

Chen,

et al. 2024

Molecules

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Neurodegenerative diseases (NDDs) are mainly induced by oxidative stress which produces excessive reactive oxygen species (ROS). Quercetin (QU) is a potent antioxidant with some effects on NDDs. This study prepared and characterized a novel glucose-modified QU liposome (QU–Glu–Lip), aiming not only to overcome QU’s poor water solubility and bioavailability but also to deliver more QU to brain tissue to enhance its neuroprotective effect. QU–Glu–Lip possessed encapsulation efficiency (EE) of 89.9%, homogenous particle sizes (116–124 nm), small PDI value (<0.3), zeta value −1.363 ± 0.437 mV, proper pH and salt stability, and proper cytotoxicity. The glucose-modified liposome penetrated the blood–brain barrier (BBB) mediated via the glucose transporter 1 (GLUT1) and was taken by neuronal cells more efficiently than liposome without glucose, according to bEnd.3 and PC12 cell tests. QU–Glu–Lip attenuated H2O2-induced oxidative damage to PC12 with higher cell viability (88.42%) and lower intracellular ROS compared to that of QU. QU–Glu–Lip had higher brain target ability and delivered more QU to neuronal cells, effectively exerting the antioxidative neuroprotection effect. There is potential for the QU–Glu–Lip application for more effective treatment of NDDs.

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“…42 Inhibition of apoptosis is the main mechanism underlying the protective effect of a variety of traditional Chinese medicinal compounds on PC12 cells. 40,43 Previous studies have shown that KPL treats a variety of diseases, such as osteoporosis, 44 knee osteoarthritis, 45 and ischemia-reperfusion injury, 13,46 by inhibiting apoptosis. Research indicates that there are 3 main apoptotic pathways: the mitochondrial pathway, the endoplasmic reticulum pathway, and the death receptors pathway.…”

Section: Discussionmentioning

confidence: 99%

“…PC12 cells exposed to H 2 O 2 are often used as a neurological injury model. [39][40][41] Therefore, we used the PC12 cell line to mimic neural cell damage after SCI and to further explore the mechanism of KPL in the treatment of SCI. The CCK-8 assay was used to determine the half-maximal inhibitory concentration of H 2 O 2 and to assess the effect of KPL or both KPL and H 2 O 2 on cell viability.…”

Section: Discussionmentioning

confidence: 99%

Kaempferol Protects Against Apoptosis in PC12 Cells Exposed to Hydrogen Peroxide by Activating Akt1

Kang

Zhang

et al. 2023

Natural Product Communications

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Objective Spinal cord injury (SCI) is a debilitating disease that cannot be cured at present. Kaempferol (KPL) has proven neuroprotective, antioxidant, and antibacterial properties. However, the neuroprotective effect of KPL on SCI and its potential mechanism are still unclear. Methods Network pharmacology and molecular docking were used to determine the potential mechanisms of KPL on SCI. In vitro studies were used to validate the results. Hydrogen peroxide (H2O2)-exposed PC12 cells were pretreated with or without KPL. The safe concentrations of KPL, H2O2, and MK2206 in PC12 cells were determined via cell counting kit-8 assays. Then, terminal deoxynucleotidyl transferase dUTP nick end labeling staining and Annexin V-FITC/PI double staining were used to detect apoptotic cells. In addition, Western blotting was utilized to measure apoptosis-related gene and protein expression levels. Results Network pharmacology and molecular docking data demonstrated an interaction between KPL and Akt1 and identified apoptosis as a potential target of the KPL/Akt1 complex. The data verified that KPL could inhibit mitochondrial apoptosis in vivo by inducing Akt1 phosphorylation. Subsequently, MK2206, a pharmacological Akt1 inhibitor, was used to assess the role of Akt1 in this process, and we found that MK2206 attenuated the antiapoptotic effect of KPL on PC12 cells. Conclusion This study demonstrates that KPL could inhibit mitochondrial apoptosis by inducing the phosphorylation of Akt1, suggesting that KPL is a very promising candidate for SCI treatment.

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Neuroprotective effects of Tiaogeng decoction against H2O2-induced oxidative injury and apoptosis in PC12 cells via Nrf2 and JNK signaling pathways

Cited by 10 publications

References 59 publications

Thermosensitive Hydrogel Incorporating Prussian Blue Nanoparticles Promotes Diabetic Wound Healing via ROS Scavenging and Mitochondrial Function Restoration

Thermosensitive Hydrogel Incorporating Prussian Blue Nanoparticles Promotes Diabetic Wound Healing via ROS Scavenging and Mitochondrial Function Restoration

A Novel Quercetin Encapsulated Glucose Modified Liposome and Its Brain-Target Antioxidative Neuroprotection Effects

Kaempferol Protects Against Apoptosis in PC12 Cells Exposed to Hydrogen Peroxide by Activating Akt1

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