Neuroprotective effects on amyloid-beta induced cytotoxicity of Pandanus clementis Merr

Abstract

The present study determined the neuroprotective potential of the alcoholic and aqueous extracts of Pandanus clementis Merr. (Pandanaceae) to protect the neuroblastoma SH-SY5Y cells against amyloid-beta 1-42 (Aβ) cytotoxicity. Inhibition of Aβ aggregation was determined by Thioflavin T (ThT) assay, and in vitro neuroprotective cell viability, intracellular reactive oxygen species (ROS), and mitochondrial membrane potential (MMP) were evaluated with human neuroblastoma SH-SY5Y cells insulted with Aβ. Chromatographic separation on the alcoholic extract yielded known phytosterols. Results showed that pretreatment of the SH-SY5Y cells with the P. clementis extracts increased cell viability and MMP, and decreased ROS, suggesting protective effects. Hence, P. clementis extract has promising neuroprotective therapeutic potential.

Effects of Cinnamon Essential Oil on Oxidative Damage and Outer Membrane Protein Genes of Salmonella enteritidis Cells

“…Referring to the method of Tan and Ramazani et al, we used the SOD kit to measure the SOD activity of the sample [ 28 , 29 ]. First, the bacterial suspension cultured to the logarithmic growth phase was added with different concentrations (1/2 MIC, MIC) of CEO to treatment for 0, 2, 4, 6, and 8 h. Then, the pellets were subject to centrifugation, washed with PBS and resuspended, and then sonicated, and centrifuged at 4 °C to keep the supernatant.…”

Pandanus amaryllifolius Exhibits In Vitro Anti-Amyloidogenic Activity and Promotes Neuroprotective Effects in Amyloid-β-Induced SH-SY5Y Cells

“…ThT assay was used to evaluate the inhibition of Aβ aggregation of the P. amaryllifolius extracts and phenol red (positive control) as previously described [ 21 , 22 , 23 ]. Briefly, Aβ42 (Aggresure™ AnaSpec, Fremont, CA, USA) in PBS was mixed with or without the P. amaryllifolius extracts or phenol red for 24 h at 37 °C.…”

“…SH-SY5Y cells (1 × 10 4 cells/well) were plated in 96-well plate and acclimatized for 24 h. Then, the cells were treated with 50, 20, 10, and 1 μg/mL P. amaryllifolius extracts for 24 h. After treatment, cells were washed with PBS, followed by the addition of 100 μL fresh media and incubation for 30 min. The cell viability was assessed following the ATP Luminescence (CellTiter-Glo ® ) (Promega, Madison, WI, USA) method as previously described [ 22 , 23 ]. The cell viability was expressed as % of the control cells (untreated).…”

“…For the neuroprotective experiment, SH-SY5Y cells (1 × 10 4 cells/well) were sub-cultured in 96-well plate and incubated for 24 h. After incubation, cells were then treated with the 20, 10, and 1 μg/mL P. amaryllifolius extracts for 6 h. This was followed by Aβ (10 μM) treatment for 24 h. The % cell viability was determined in triplicate experiments using the ATP Luminescence (CellTiter-Glo ® ) method as previously described [ 22 , 23 ].…”

“…In our continuing search for Philippine medicinal plants and their constituents with inhibitory effects against Aβ toxicity in vitro [ 19 , 20 , 21 , 22 , 23 ], we herein describe the neuroprotective effects and inhibition of Aβ aggregations and oligomerizations of P. amaryllifolius crude alcoholic (CAE) and crude base (CBE) extracts. The unprecedented isolation of nicotinamide from P. amaryllifolius , as a potential bioactive constituent of the crude base extract, is also reported.…”