Neuroprotective potential of fisetin in an experimental model of spinal cord injury: via modulation of NF-κB/IκBα pathway

Abstract

Supplemental Digital Content is available in the text.

Discovering the Effects of Fisetin on NF-κB/NLRP-3/NRF-2 Molecular Pathways in a Mouse Model of Vascular Dementia Induced by Repeated Bilateral Carotid Occlusion

“…Group 4: VaD + Fisetin = same as the VaD + veh group, but Fisetin at a dose of 40 mg/kg orally, dissolved in 1% aqueous solution of dimethyl sulfoxide (DMSO), was administered 24 h after the surgical procedure and once a day for 15 days thereafter (N = 12). The dose and the route of administration of Fisetin were chosen based on a previous study [ 26 ]. Because no significant difference was found between the sham + vehicle and sham + Fisetin, only data regarding the sham + vehicle groups were shown.…”

“…- Novel object recognition test (NOR): This model was performed as previously described [ 26 ]. The exploration time for the animal to investigate the new object was reported.…”

“…- Morris water maze (MWM): The MWM approach was used to investigate spatial learning and memory. In navigation trials (3 trials per day for 5 days), the mice’s escape latency (the time it took them to identify and stand on a platform under water) was assessed, and the frequency time around and within the platform quadrant was measured in probe trial (at 6th day) [ 26 ].…”

“…Histopathological examination with hematoxylin and eosin staining was performed as previously described [ 26 , 27 , 28 , 29 ]. The brains were collected rapidly and preserved in 4% formalin.…”

“…The hippocampal CA1 and CA3 areas were then examined using a Leica DM6 microscope and Leica LAS X Navigator software (Leica Microsystems SpA, Milan, Italy). Ischemic neuronal damage was scored as previously [ 26 ]. All the histological studies were completed in blind.…”