Neuroregenerative effects of polyethylene glycol and FK-506 in a rat model of sciatic nerve injury

Paskal, Adriana M.; Paskal, Wiktor; Pietruski, Piotr; Kusmierczyk, Zofia; Jankowska‐Steifer, Ewa; Andrychowski, Jarosław; Włodarski, Paweł

doi:10.1016/j.bjps.2019.10.011

Cited by 6 publications

(5 citation statements)

References 36 publications

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“…For more precise morphometrical evaluation, samples from each group were stained with Weigert-Pal (WP) method according to the protocol described by Kiernan et al to stain myelin sheaths [ 51 ]. Quantitative assessment was performed for sections B. Nerve fibers were identified with the aid of a semi-automatic analysis tool—CellProfiler and adjusted protocol reported by Paskal et al [ 52 ]. Nerve fiber density was described as the number of fibers in mm 2 (axons/mm 2 ) in NF-200 staining and WP staining.…”

Section: Methodsmentioning

confidence: 99%

Bioactive Nanofiber-Based Conduits in a Peripheral Nerve Gap Management—An Animal Model Study

Dębski

Kijeńska‐Gawrońska

Zołocińska

et al. 2021

IJMS

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The aim was to examine the efficiency of a scaffold made of poly (L-lactic acid)-co-poly(ϵ-caprolactone), collagen (COL), polyaniline (PANI), and enriched with adipose-derived stem cells (ASCs) as a nerve conduit in a rat model. P(LLA-CL)-COL-PANI scaffold was optimized and electrospun into a tubular-shaped structure. Adipose tissue from 10 Lewis rats was harvested for ASCs culture. A total of 28 inbred male Lewis rats underwent sciatic nerve transection and excision of a 10 mm nerve trunk fragment. In Group A, the nerve gap remained untouched; in Group B, an excised trunk was used as an autograft; in Group C, nerve stumps were secured with P(LLA-CL)-COL-PANI conduit; in Group D, P(LLA-CL)-COL-PANI conduit was enriched with ASCs. After 6 months of observation, rats were sacrificed. Gastrocnemius muscles and sciatic nerves were harvested for weight, histology analysis, and nerve fiber count analyses. Group A showed advanced atrophy of the muscle, and each intervention (B, C, D) prevented muscle mass decrease (p < 0.0001); however, ASCs addition decreased efficiency vs. autograft (p < 0.05). Nerve fiber count revealed a superior effect in the nerve fiber density observed in the groups with the use of conduit (D vs. B p < 0.0001, C vs. B p < 0.001). P(LLA-CL)-COL-PANI conduits with ASCs showed promising results in managing nerve gap by decreasing muscle atrophy.

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Section: Methodsmentioning

confidence: 99%

Bioactive Nanofiber-Based Conduits in a Peripheral Nerve Gap Management—An Animal Model Study

Dębski

Kijeńska‐Gawrońska

Zołocińska

et al. 2021

IJMS

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“…Tacrolimus, also known as FK506, is another macrolide that is widely used as an immunosuppressive medication [84,88]. It can cross the brain–blood barrier and exert robust neuroprotective and neuroregenerative effects in different CNS diseases such as SCI ( Table 1 ) [1,89–95]. Tacrolimus promotes neuroprotection in addition to neural and axonal regeneration associated with functional recovery in SCI rodents [96–100].…”

Section: Antibiotics With Therapeutic Effects In Scimentioning

confidence: 99%

Antibiotics with therapeutic effects on spinal cord injury: a review

Afshari

Roudsari

Lashgari

et al. 2020

Fundamemntal Clinical Pharma

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Accumulating evidence indicates that a considerable number of antibiotics exert anti‐inflammatory and neuroprotective effects in different central and peripheral nervous system diseases including spinal cord injury (SCI). Both clinical and preclinical studies on SCI have found therapeutic effects of antibiotics from different families on SCI. These include macrolides, minocycline, β‐lactams, and dapsone, all of which have been found to improve SCI sequels and complications. These antibiotics may target similar signaling pathways such as reducing inflammatory microglial activity, promoting autophagy, inhibiting neuronal apoptosis, and modulating the SCI‐related mitochondrial dysfunction. In this review paper, we will discuss the mechanisms underlying therapeutic effects of these antibiotics on SCI, which not only could supply vital information for investigators but also guide clinicians to consider administering these antibiotics as part of a multimodal therapeutic approach for management of SCI and its complications.

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“…Images of stained nerve sections were captured under 400x magni cation. Quantitative assessment was performed for sections B. Nerve ber were identi ed with the aid of semi-automatic analysis tool -CellPro ler and adjusted protocol reported by Paskal et al [18]. Nerve ber density was described as the number of bers in an mm 2 (axons/mm 2 ).…”

Section: Samples Harvest and Histological Analysesmentioning

confidence: 99%

Bioactive Nanofiber-based Conduits in a Peripheral Nerve Gap Management- an Animal Model Study.

Dębski

Kijeńska‐Gawrońska

Zołocińska

et al. 2020

Preprint

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Background: Peripheral nerve injuries (PNI) are trauma with severe squeals for patients’ quality of life. Currently, the gold standard of nerve reconstruction relies on primary coaptation. However, in case of a nerve gap, a reconstructive material for bridging is needed. Nerve conduits are an increasingly popular solution alternative to autografts or processed nerved allografts. The aim of the study was to examine the efficiency of a novel bioactive nanofiber-based tubular scaffold made of poly (L-lactic acid)-co-poly(ϵ-caprolactone), collagen, polyaniline and enriched with adipose-derived stem cells (ASCs) as a nerve conduit in a rat model.Methods: Poly (L-lactic acid)-co-poly(ϵ-caprolactone) scaffold was optimized and enriched with collagen (COL) and polyaniline (PANI) to create final (P(LLA-CL)-COL-PANI), tubular-shaped scaffold, manufactured with electrospinning technology. Parallelly, adipose tissue from 10 Lewis rats was harvested for ASC culture. 28 inbred male Lewis rats were divided into four even groups. Each animal underwent sciatic nerve transection and excision of a 10 mm nerve trunk fragment. In group A, nerve gap remained untouched, in B excised trunk was rotated and used as an autograft, in C nerve stumps were secured with P(LLA-CL)-COL-PANI conduit. In the D group gap was reconstructed with P(LLA-CL)-COL-PANI conduit enriched with ASCs. After 6-months of observation rats were sacrificed. Gastrocnemius muscles (operated and non-operated side) and reconstructed sciatic nerves were harvested for analyses. Muscles were weighted and underwent histological analysis. Nerves were processed and stained immunohistochemically with NF-200 to be analyzed with dedicated software for nerve fiber count. Results: No signs of rejection or excessive fibrosis was noted. Muscle mass ratio was highest in group B (0.77±0.05), then in C (0.74±0,04) and D (0.67± 0.07). Group A showed advanced atrophy of the muscle, each intervention prevented muscle mass decrease (p<0.0001), however, ASC addition decreased efficiency vs autograft (p<0.05). Nerve fiber count revealed a superior effect in the nerve fiber density observed in the group with the use of conduit vs autograft (D vs B p<0.0001, C vs B p<0.001). ASC added to the conduit decreased perineurium hyperplasia.Conclusion: P(LLA-CL)-COL-PANI conduits with ASC showed promising results in managing nerve gap by decreasing muscle atrophy and providing a favorable environment for peripheral nerve regeneration.

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Neuroregenerative effects of polyethylene glycol and FK-506 in a rat model of sciatic nerve injury

Cited by 6 publications

References 36 publications

Bioactive Nanofiber-Based Conduits in a Peripheral Nerve Gap Management—An Animal Model Study

Bioactive Nanofiber-Based Conduits in a Peripheral Nerve Gap Management—An Animal Model Study

Antibiotics with therapeutic effects on spinal cord injury: a review

Bioactive Nanofiber-based Conduits in a Peripheral Nerve Gap Management- an Animal Model Study.

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