Neurospheres from rat adipose-derived stem cells could be induced into functional Schwann cell-like cells in vitro

Xu, Yongfeng; Liu, Zhengshan; Liu, Lan; Zhao, Chuanzhi; Xiong, Fu; Zhou, Chang; Li, Yong; Shan, Yanchang; Peng, Funing; Zhang, Cheng

doi:10.1186/1471-2202-9-21

Cited by 98 publications

(90 citation statements)

References 36 publications

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“…In support of this, human ASCs can form nestin-positive neurospheres that, upon dissociation, express characteristic SC markers like S100, GFAP, and the p75 NGF receptor [123]. Similar markers have also been seen in rat ASCs induced with mixture of glial growth factors [130,131]. Moreover, SC-induced ASCs also appear to possess functional capacity, demonstrating the ability to stimulate neurite outgrowth of dorsal root ganglia neurons (DRGNs) under coculture [123,132], NG108-12 motor neurons [130], and SH-SY5Y neuroblastoma cells [131].…”

Section: Increased Potential Of Ascs: In Vitro Ectodermal Capacitymentioning

confidence: 66%

Adipose-Derived Stem Cells in Tissue Regeneration: A Review

2013

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In 2001, researchers at the University of California, Los Angeles, described the isolation of a new population of adult stem cells from liposuctioned adipose tissue. These stem cells, now known as adipose-derived stem cells or ADSCs, have gone on to become one of the most popular adult stem cells populations in the fields of stem cell research and regenerative medicine. As of today, thousands of research and clinical articles have been published using ASCs, describing their possible pluripotency in vitro, their uses in regenerative animal models, and their application to the clinic. This paper outlines the progress made in the ASC field since their initial description in 2001, describing their mesodermal, ectodermal, and endodermal potentials both in vitro and in vivo, their use in mediating inflammation and vascularization during tissue regeneration, and their potential for reprogramming into induced pluripotent cells.

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Section: Increased Potential Of Ascs: In Vitro Ectodermal Capacitymentioning

confidence: 66%

Adipose-Derived Stem Cells in Tissue Regeneration: A Review

2013

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“…Both populations presented identical cell size, doubling times and karyotype stability, differing only in morphology with rounder cells in the sub-odontoblastic compartment versus spindle-shaped cells with long processes in the perivascular one [239]. Pre-clinical experiments have demonstrated that stem cells show promising results in differentiation into neuronal-like cells [226,228,232,233,240] and their secretion of growth factors [229,241,242]. Shi and colleagues [243] stated the potential of glia-derived neurotrophic factor expressing neural stem cells in the regeneration of facial nerve gap in rats.…”

Section: Cellular Systemsmentioning

confidence: 99%

“…Other sources of stem cells have been used in peripheral nerve injuries, such as adipose-derived stem cells [203,206,212,[226][227][228][229][230][231] and dental pulp stem cells (DPSCs) [232][233][234][235][236]. Dental pulp stem cells have also demonstrated differentiation capacity towards multiple other mesodermal and endodermal lineages, under appropriate conditions: adipogenic, osteo/dentinogenic, chondrogenic, neurogenic, endothelial, myofibroblastic [237] and hepatocytes [238].…”

Section: Cellular Systemsmentioning

confidence: 99%

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

Pedrosa¹,

Caseiro²,

Santos³

et al. 2017

Scaffolds in Tissue Engineering - Materials, Technologies and Clinical Applications

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Human adult peripheral nerve injuries are a high incidence clinical problem that greatly affects patients' quality of life. Although peripheral nervous system has intrinsic regenerative capacity, this occurs in an incomplete or poorly functional manner. When a nerve fiber loses its continuity with consequent damage of the basal lamina tubes, axon spontaneous regeneration is disorganized and mismatched. These phenomena translate in an inadequate nerve functional recovery and consequent musculoskeletal incapacity. Nerve grafts still remain the gold standard in peripheral injuries treatment. However, this approach contains its disadvantages such as the necessity of primary surgery to harvest the autografts, loss of a functional nerve, donor site morbidity and longer surgery procedures. Therefore, biomaterials and tissue engineering can provide efficient resources and alternatives to nerve injury repair not only by the development of biocompatible structures but also, introducing neurotrophic factors and cellular systems to stimulate optimum clinical outcome. In this chapter, a comprehensive state-of-the art picture of tissue-engineered nerve grafts scaffolds, their application in nerve regeneration along with latest advances in peripheral nerve repair and future perspectives will be discussed, including our own large experience in this field of knowledge.

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“…Unlike BMSCs, donor age and site of harvest does not seem to significantly influence the therapeutic effect of ADSCs [50] . Multiple neuronal and SC markers have been expressed from differentiated ADSCs [7,30,[95][96][97] . The expression of myelin protein zero, peripheral myelin protein-22 and myelin basic protein also suggest the retention of myelinating capability [98] .…”

Section: Bone Marrow Derived Stem Cellsmentioning

confidence: 99%

Augmenting peripheral nerve regeneration using stem cells: A review of current opinion

Fairbairn

Meppelink

Ng-Glazier

et al. 2015

WJSC

128

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Outcomes following peripheral nerve injury remain frustratingly poor. The reasons for this are multifactorial, although maintaining a growth permissive environment in the distal nerve stump following repair is arguably the most important. The optimal environment for axonal regeneration relies on the synthesis and release of many biochemical mediators that are temporally and spatially regulated with a high level of incompletely understood complexity. The Schwann cell (SC) has emerged as a key player in this process. Prolonged periods of distal nerve stump denervation, characteristic of large gaps and proximal injuries, have been associated with a reduction in SC number and ability to support regenerating axons. Cell based therapy offers a potential therapy for the improvement of outcomes following peripheral nerve reconstruction. Stem cells have the potential to increase the number of SCs and prolong their ability to support regeneration. They may also have the ability to rescue and replenish populations of chromatolytic and apoptotic neurons following axotomy. Finally, they can be used in non-physiologic ways to preserve injured tissues such as denervated muscle while neuronal ingrowth has not yet occurred. Aside from stem cell type, careful consideration must be given to differentiation status, how stem cells are supported following transplantation and how they will be delivered to the site of injury. It is the aim of this article to review current opinions on the strategies of stem cell based therapy for the augmentation of peripheral nerve regeneration.

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Neurospheres from rat adipose-derived stem cells could be induced into functional Schwann cell-like cells in vitro

Cited by 98 publications

References 36 publications

Adipose-Derived Stem Cells in Tissue Regeneration: A Review

Adipose-Derived Stem Cells in Tissue Regeneration: A Review

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

Augmenting peripheral nerve regeneration using stem cells: A review of current opinion

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