Neurospora crassa homologue of Neuronal Calcium Sensor-1 has a role in growth, calcium stress tolerance, and ultraviolet survival

Deka, Rekha; Kumar, Rakesh; Tamuli, Ranjan

doi:10.1007/s10709-011-9592-y

Cited by 18 publications

(13 citation statements)

References 66 publications

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“…Deletion of cse-1 in N. crassa results in a mutant that is viable, but sensitive to calcium stress and ultraviolet light, and which shows slightly impaired growth [33]. Similar to NCS-1 and Frq1p, CSE1 harbors a consensus signal for N-terminal myristoylation and four EF-hand domains (PF00036) predicted to be involved in calcium binding [32], [34], [35].…”

Section: Resultsmentioning

confidence: 99%

“…We reasoned that these attributes would likely be conserved in N. crassa . We first focused on the role of calcium; the Δ cse-1 mutant shows growth sensitivity to excess calcium, as well as to calcium depletion [33]. We therefore hypothesized that calcium could be required for chemotropic interactions between N. crassa germlings, and to test this, we assayed fusion of wild-type germlings on growth medium depleted of Ca 2+ .…”

Section: Resultsmentioning

confidence: 99%

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Genome Wide Association Identifies Novel Loci Involved in Fungal Communication

et al. 2013

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Understanding how genomes encode complex cellular and organismal behaviors has become the outstanding challenge of modern genetics. Unlike classical screening methods, analysis of genetic variation that occurs naturally in wild populations can enable rapid, genome-scale mapping of genotype to phenotype with a medium-throughput experimental design. Here we describe the results of the first genome-wide association study (GWAS) used to identify novel loci underlying trait variation in a microbial eukaryote, harnessing wild isolates of the filamentous fungus Neurospora crassa. We genotyped each of a population of wild Louisiana strains at 1 million genetic loci genome-wide, and we used these genotypes to map genetic determinants of microbial communication. In N. crassa, germinated asexual spores (germlings) sense the presence of other germlings, grow toward them in a coordinated fashion, and fuse. We evaluated germlings of each strain for their ability to chemically sense, chemotropically seek, and undergo cell fusion, and we subjected these trait measurements to GWAS. This analysis identified one gene, NCU04379 (cse-1, encoding a homolog of a neuronal calcium sensor), at which inheritance was strongly associated with the efficiency of germling communication. Deletion of cse-1 significantly impaired germling communication and fusion, and two genes encoding predicted interaction partners of CSE1 were also required for the communication trait. Additionally, mining our association results for signaling and secretion genes with a potential role in germling communication, we validated six more previously unknown molecular players, including a secreted protease and two other genes whose deletion conferred a novel phenotype of increased communication and multi-germling fusion. Our results establish protein secretion as a linchpin of germling communication in N. crassa and shed light on the regulation of communication molecules in this fungus. Our study demonstrates the power of population-genetic analyses for the rapid identification of genes contributing to complex traits in microbial species.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Genome Wide Association Identifies Novel Loci Involved in Fungal Communication

et al. 2013

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“…In T. reesei it was suggested that ENVOY and PDE are interconnected and in concert they regulate the light-dependent G-protein signaling pathway with cellulase production as an output pathway (619). The NCS of N. crassa shows functions in growth, calcium stress, and UV tolerance (863). In the fission yeast Schizosaccharomyces pombe, NCS is required for nutrition-independent sexual development and is involved in sporulation (864).…”

Section: Genomes Of T Reesei T Virens and T Atroviride Respectimentioning

confidence: 99%

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Schmoll

Dattenböck

Carreras-Villaseñor

et al. 2016

Microbiol Mol Biol Rev

166

195

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SUMMARYThe genusTrichodermacontains fungi with high relevance for humans, with applications in enzyme production for plant cell wall degradation and use in biocontrol. Here, we provide a broad, comprehensive overview of the genomic content of these species for “hot topic” research aspects, including CAZymes, transport, transcription factors, and development, along with a detailed analysis and annotation of less-studied topics, such as signal transduction, genome integrity, chromatin, photobiology, or lipid, sulfur, and nitrogen metabolism inT. reesei,T. atroviride, andT. virens, and we open up new perspectives to those topics discussed previously. In total, we covered more than 2,000 of the predicted 9,000 to 11,000 genes of eachTrichodermaspecies discussed, which is >20% of the respective gene content. Additionally, we considered available transcriptome data for the annotated genes. Highlights of our analyses include overall carbohydrate cleavage preferences due to the different genomic contents and regulation of the respective genes. We found light regulation of many sulfur metabolic genes. Additionally, a new Golgi 1,2-mannosidase likely involved inN-linked glycosylation was detected, as were indications for the ability ofTrichodermaspp. to generate hybrid galactose-containingN-linked glycans. The genomic inventory of effector proteins revealed numerous compounds unique toTrichoderma, and these warrant further investigation. We found interesting expansions in theTrichodermagenus in several signaling pathways, such as G-protein-coupled receptors, RAS GTPases, and casein kinases. A particularly interesting feature absolutely unique toT. atrovirideis the duplication of the alternative sulfur amino acid synthesis pathway.

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“…Other Ca 2+ -related deletion strains present altered resistance to staurosporine, including Δ cse-1 , Δ camk-4 and Δ stk-16 . Knockout of cse-1 has been shown to lead to impaired germling communication and fusion 43, as well as increased sensitivity to UV irradiation and Ca 2+ 44. A previous study has shown that disruption of camk-4 results in benomyl resistance, while disruption of stk-16 leads to increased sensitivity to fludioxonil, sodium chloride and benomyl 45.…”

Section: Discussionmentioning

confidence: 99%

Extracellular calcium triggers unique transcriptional programs and modulates staurosporine-induced cell death in Neurospora crassa

Gonçalves¹,

Monteiro

Lucchi

et al. 2014

MIC

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Alterations in the intracellular levels of calcium are a common response to cell death stimuli in animals and fungi and, particularly, in the Neurospora crassa response to staurosporine. We highlight the importance of the extracellular availability of Ca2+ for this response. Limitation of the ion in the culture medium further sensitizes cells to the drug and results in increased accumulation of reactive oxygen species (ROS). Conversely, an approximately 30-fold excess of external Ca2+ leads to increased drug tolerance and lower ROS generation. In line with this, distinct staurosporine-induced cytosolic Ca2+ signaling profiles were observed in the absence or presence of excessive external Ca2+. High-throughput RNA sequencing revealed that different concentrations of extracellular Ca2+ define distinct transcriptional programs. Our transcriptional profiling also pointed to two putative novel Ca2+-binding proteins, encoded by the NCU08524 and NCU06607 genes, and provides a reference dataset for future investigations on the role of Ca2+ in fungal biology.

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Neurospora crassa homologue of Neuronal Calcium Sensor-1 has a role in growth, calcium stress tolerance, and ultraviolet survival

Cited by 18 publications

References 66 publications

Genome Wide Association Identifies Novel Loci Involved in Fungal Communication

Genome Wide Association Identifies Novel Loci Involved in Fungal Communication

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Extracellular calcium triggers unique transcriptional programs and modulates staurosporine-induced cell death in Neurospora crassa

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