Neurotensin and prostaglandin interactions in smooth muscle of the guinea pig stomach

Katsoulis, Stavros; Conlon, J. Michael

doi:10.1016/0014-2999(88)90074-x

Cited by 8 publications

(5 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The NT-induced activation of guinea pig gastric muscle was insensitive to atropine, phentolamine, (7). Similar direct myogenic effects of NT, sensitive to indomethacin, have been reported for the isolated colon (21,22) and the isolated rat portal vein (23).…”

Section: And Prostaglandinssupporting

confidence: 77%

“…In the circular Paper received November 9 , 1989; revised November 13, 1990. 1043451 891 00002F muscle of the ileum the response was inhibitory and was produced by myogenic and neurogenic mechanisms (6). In guinea pig gastric muscle (longitudinal corpus) NT produced an initial small relaxation followed by a sustained contraction, an effect which was not significantly modified by TTX or muscarinic or adrenergic antagonists (7).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Neurotensin on Gastric Smooth Muscle of Dog and Guinea Pig

Mandrek

Milenov

1991

Neurogastroenterology Motil

View full text Add to dashboard Cite

Mechanical activity was recorded in muscle preparations isolated from different regions of dog and guinea pig stomach. Neurotensin (10−11 to 10−8 mol/liter) produced three types of responses in canine gastric muscle: a small activation of tone infundic strips and in longitudinal strips from corpus and antrum; a transient inhibition of the rhythmic activity in circular strips from corpus, antrum, and outer pylorus; and a strong activation in the strips from inner pylorus. In guinea pig gastric muscle strong tonic responses were observed in circular fundic muscle strips, whereas in all other preparations the responses were negligible. All these effects were insensitive to atropine, dimethpyrindene, and tetrodotoxin. The neurotensin effect on strips from guinea pig stomach was strongly reduced in the presence of indomethacin or cortisone and markedly potentiated in the presence of arachidonic acid or prostaglandin F2α. contrast, the excitatory effect of neurotensin on canine inner pylorus was potentiated during indomethacin treatment.

show abstract

Section: And Prostaglandinssupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Effects of Neurotensin on Gastric Smooth Muscle of Dog and Guinea Pig

Mandrek

Milenov

1991

Neurogastroenterology Motil

View full text Add to dashboard Cite

show abstract

“…In the guinea pig stomach, the presence of inhibitory [6] or excitatory [7] neurotensin receptors on smooth muscle cells have been suggested. This study dem onstrated the absence of a direct contractile response to neurotensin in isolated gastric smooth muscle cells, and that neurotensin has a direct inhibitory effect on carbachol-induced gastric smooth muscle cell contraction, which an IC50 value of 5 nM.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, the inhibitory effect of neurotensin on guinea pig [17], rat [13,15] and porcine [18] small intestine, and guinea pig colon [13] has been observed. Furthermore, a biphasic effect of neurotensin (initial relaxation followed by sustained contraction) on guinea pig stomach [6,13], and small intestine of guinea pigs [13. 19.…”

Section: Discussionmentioning

confidence: 99%

“…Neurotensin causes inhibition of motility, resulting in a delay in gastric emptying [3][4][5], Katsoulis and Conlon [6] reported a biphasic effect (initial relaxation followed by sustained contraction) of neurotensin on longitudinal muscle strips prepared from guinea pig stomach and sug gested the presence of inhibitory neurotensin receptors on smooth muscle cells. On the other hand.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Direct Inhibitory Effect of Neurotensin on Isolated Gastric Smooth Muscle Cells of Guinea Pig via the Cyclic GMP System

Chijiíwa

Kawakami²,

Misawa³

et al. 1993

Digestion

View full text Add to dashboard Cite

Smooth muscle cells isolated from the gastric muscle layers of the guinea pig were used to determine whether neurotensin can inhibit the contractile response produced by 10^-6M carbachol by exerting a direct action on muscle cells. In addition, the inhibitory effect of 2´,5´ -dideoxyadenosine, an inhibitor of adenylate cyclase, and methylene blue, an inhibitor of guanylate cyclase, on the neurotensin-induced relaxation of gastric smooth muscle cells were examined. Neurotensin inhibited the contractile response produced by carbachol in a dose-dependent manner, with an IC50 value of 5 nM. 2´,5´ -Dideoxyadenosine did not have any significant effect on the neurotensin-induced relaxation. On the other hand, methylene blue significantly inhibited the relaxation produced by neurotensin. These results strongly suggest that the action of neurotensin is mediated by cyclic GMP.

show abstract

Calcitonin gene-related peptides relax guinea pig and rat gastric smooth muscle

Katsoulis

Conlon

1989

European Journal of Pharmacology

View full text Add to dashboard Cite

Neurotensin and prostaglandin interactions in smooth muscle of the guinea pig stomach

Cited by 8 publications

References 24 publications

Effects of Neurotensin on Gastric Smooth Muscle of Dog and Guinea Pig

Effects of Neurotensin on Gastric Smooth Muscle of Dog and Guinea Pig

Direct Inhibitory Effect of Neurotensin on Isolated Gastric Smooth Muscle Cells of Guinea Pig via the Cyclic GMP System

Calcitonin gene-related peptides relax guinea pig and rat gastric smooth muscle

Contact Info

Product

Resources

About