Neurotoxic effect of Caulerpa racemosa var. cylindracea by neurite inhibition on the neuroblastoma cell line

Kurt, Oğuz; Özdal-Kurt, F.; Tuğlu, Mehmet İbrahim; Deliloğlu-Gürhan, S İsmet; Öztürk, Mehmet

doi:10.1134/s1063074009040105

Cited by 8 publications

(2 citation statements)

References 35 publications

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“…MTT solution was removed, 200 µL of DMSO was added to each well, and spectrophotometric measurements were performed using a microplate reader at 570 nm with a 690 nm reference filter. 29…”

Section: -(45-dimethylthiazol-2-yl)-25diphenyltetrazolium Bromidementioning

confidence: 99%

In vitro investigation of the effect of matrix molecules on the behavior of colon cancer cells under the effect of geldanamycin derivative

et al. 2017

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The chaperone-binding drug, 17-allylamino-17-demethoxygeldanamycin, has recently come into clinical use. It is a derivative of geldanamycin, an ansamycin benzoquinone antibiotic with anti-carcinogenic effect. Understanding the effect of this drug on the cancer cells and their niche is important for treatment. We applied 17-allylamino-17-demethoxygeldanamycin to colon cancer cell line (Colo 205) on matrix molecules to investigate the relationship of apoptosis with terminal deoxynucleotidyl transferase dUTP nick end labeling immunocytochemistry and related gene expression. We used laminin and collagen I for matrix molecules and vascular endothelial growth factor for angiogenic structure. We also examined apoptosis-related signaling pathway including mitochondrial proteins, cytochrome c, Bcl-2, caspase-9, Apaf-1 expression using real-time polymerase chain reaction. There was clear effect of 17-allylamino-17-demethoxygeldanamycin that killed more cells on tissue culture plastic compared to matrix molecules. The IC value was 0.58 µg/mL for tissue culture plastic compared with 0.64 µg/mL for laminin and 0.75 µg/mL for collagen I. The analyses showed that more cells on matrix molecules underwent apoptosis compared to that on tissue culture plastic. Apoptosis-related gene expression was similar in which Bcl-2 expression decreased and proapoptotic gene expression of the cells on matrix molecules increased compared to that on tissue culture plastic. However, the application of 17-allylamino-17-demethoxygeldanamycin was more effective for the cells on collagen I compared to the cells on laminin. There was also a decrease in angiogenesis as shown by the vascular endothelial growth factor staining. This was more pronounced by coating of the tissue culture plastic with matrix molecules. Our results supported the anti-cancer effect of 17-allylamino-17-demethoxygeldanamycin, and this effect depended on matrix molecules. This effect occurs through apoptosis, and related genes were also altered. All these genes may serve for novel target under the effect of matrix substrate. However, correct interpretation of the results requires further studies.

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“…MTT solution was removed, 200 µL of DMSO was added to each well, and spectrophotometric measurements were performed using a microplate reader at 570 nm with a 690 nm reference filter. 29…”

Section: -(45-dimethylthiazol-2-yl)-25diphenyltetrazolium Bromidementioning

confidence: 99%

In vitro investigation of the effect of matrix molecules on the behavior of colon cancer cells under the effect of geldanamycin derivative

et al. 2017

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“…Nevertheless, its neurotoxic effect is a factor to be considered as multi functional agents especially in neuronal metabolism (Kurt 2009). …”

Section: Toxicitymentioning

confidence: 99%

Opportunities and Challenges for Developing Caulerpa Racemosa as Functional Foods

Fithriani

2015

KLS

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The higher public awareness of the health in community would made the view of some people for food has shifted, not only as relieving hunger, but also can provide a real impact for health. This phenomenon has resulted a term what is called functional food'. Functional food is defined as food which contain active compounds that play a role in increasing endurance, minimize the risk of attack of certain diseases and to provide maximum health benefits. One of marine resource that have a potency to develop as functional foods is Caulerpa racemosa. Caulerpa racemosa is one of green seaweed that grows naturally in the waters of Indonesia. Caulerpa racemosa found growing on coral substrate or a substrate of sand-rubble. Caulerpa racemosa is edible or can be consumed by humans. Caulerpa racemosa from Indonesia contains high insoluble dietary fiber. Insoluble dietary fiber contains cellulose and hemicellulose, who plays an important role in preventing constipation, colitis and hemmoroid. In other hand Caulerpa racemosa can also produce secondary metabolites, including antioxidants. Antioxidants are substances that are capable of slowing or preventing the oxidation processes that are beneficial to health.

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Marine macroalga Caulerpa: role of its metabolites in modulating cancer signaling

2019

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Neurotoxic effect of Caulerpa racemosa var. cylindracea by neurite inhibition on the neuroblastoma cell line

Cited by 8 publications

References 35 publications

In vitro investigation of the effect of matrix molecules on the behavior of colon cancer cells under the effect of geldanamycin derivative

In vitro investigation of the effect of matrix molecules on the behavior of colon cancer cells under the effect of geldanamycin derivative

Opportunities and Challenges for Developing Caulerpa Racemosa as Functional Foods

Marine macroalga Caulerpa: role of its metabolites in modulating cancer signaling

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