Neurotransmitter Amino Acids as Modulators of Biological Processes of Spermatozoa

Boldizsár, H; Wekerle, L.; Vén, E.; Sarlós, P.; Barna, Judit

doi:10.1007/978-3-642-76915-3_14

Cited by 5 publications

(6 citation statements)

References 16 publications

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“…Results from other studies indicated a role for GABA in mammalian fertilization process [13], [16]. Aanesen et al in 1996 has found the presence of GABA uptake in human sperm and suggested that GABA transporter may be involved in regulating the action of GABA on sperm [8], [9].…”

Section: Resultsmentioning

confidence: 97%

“…GABA transporters are members of a large family characterized structurally by a homology in amino acid sequence and functionally by their co-transporting of Na + and Cl -across the cell membrane [11]. It has been suggested as early as 1982 that in fowl spermatozoa there existed a carrier-mediated transport of GABA [12], and the presence of glutamate transporter, another amino acid transporter, was also reported recently on boar spermatozoa [13].…”

Section: Introductionmentioning

confidence: 99%

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Identification of γ-aminobutyric acid transporter (GAT1) on the rat sperm

Yuan

2000

Cell Res

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Some recent studies indicated that GABAergic system is involved in mammalian sperm acrosome reaction (AR), but direct evidence pertaining to the expression of gat1 in mammalian sperm is not yet demonstrated. In this study, we evaluated the presence of 67kDa GAT1 protein and mRNA in rat testis by Western blotting and reverse transcription-polymerase chain reaction. Meanwhile, immunohistochemical and immunofluorescent analyses also identified GAT1 protein on the elongated spermatid and sperm. These results indicated that rat testis is a novel site of gat1 expression. Further studies should be taken to explore the role of GAT1 protein on sperm acrosome reaction.

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Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Identification of γ-aminobutyric acid transporter (GAT1) on the rat sperm

Yuan

2000

Cell Res

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“…The GABA A R is a pentamer composed of various subunits, and the GABA B R is a heterodimer of GABA B(1) and GABA B(2) (Jones et al, 1998; Kaupmann et al, 1998; White et al, 1998; Kuner et al, 1999). GABA and GABA A R are found in many nonneuronal peripheral tissues, including both male and female reproductive structures, such as testis (Erdö et al, 1983; Boldizsar et al, 1992; Frungieri et al, 1996; Akinci and Schofield, 1999), epididymis (Erdö et al, 1983; Frungieri et al, 1996), vas deferens (Erdö et al, 1983), ovary (del Rio and Caballero, 1980; Apud et al, 1984; Erdö and László, 1984; Tanaka, 1985; Amenta et al, 1986; Gimeno et al, 1986), oviduct (Erdö et al, 1982; Martin del Rio and Sierra Lopez, 1983; Celotti et al, 1986; Orensanz et al, 1986; Erdö and Wolff, 1990; László et al, 1992), and uterus (Erdö, 1984; Gimeno et al, 1986; Erdö et al, 1989). In peripheral nonneuronal tissues, the putative functions of GABA differ according to the cell type.…”

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confidence: 99%

Cellular Localization of GABA and GABA_B Receptor Subunit Proteins During Spermiogenesis in Rat Testis

Kanbara

Okamoto

Nomura

et al. 2005

Journal of Andrology

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ABSTRACT:The GABAergic system, a major inhibitory regulator in the central nervous system, may also play important roles in peripheral nonneuronal tissues and cells. Recent studies showed that GA-BA B receptor is expressed in testis and sperm. To understand the role of the GABAergic system in spermiogenesis, we examined cellular localization of GABA and GABA B receptor subunits in rat spermatids by immunocytochemistry. Immunoreactivity for GABA was detected around acrosomal granules of spermatids during the Golgi and cap phases. GABA B(1) immunoreactivity was observed in the acrosomal vesicle of spermatids in Golgi phase, and during cap phase, this reactivity expanded to the entire region of the acrosome covering the nuclear membrane. The level of reactivity decreased gradually with maturation of spermatids. In contrast, GABA B(2) immunoreactivity was not observed in spermatids during Golgi phase but was detected in the equatorial region during cap phase. Both GABA immunoreactivity and GABA B(2) immunoreactivity were transferred to the residual cytoplasm during the release of spermatozoa. Electron microscopic immunocytochemistry revealed that, during cap phase, GABA and GABA B(1) were distributed within the whole acrosomal vesicle but not in the acrosomal granule. GABA B(2) immunoreactivity was observed in the narrow space between the inner acrosomal and nuclear membrane and was limited to the equatorial region of the spermatid head. These results indicate that the GABAergic system might be involved in regulation of spermiogenesis.

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“…Previously, others had used 36 C1-to demonstrate that 1 mM GABA caused an influx of Cl-in uncapacitated boar sperm [49]. The fluorescent Cl-probe 6-methoxy-N-ethylquinolonium (MEQ) provides another method for analyzing cellular Cl-flux [50].…”

Section: The Sperm Gabaa Receptor-like/ci-channel and The Progesteronmentioning

confidence: 99%

Amino Acid Neurotransmitter Receptor/Chloride Channels of Mammalian Sperm and the Acrosome Reaction1

Meizel¹

1997

Biology of Reproduction

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Two different amino acid neurotransmitter receptor/Cl-channels, a gamma-aminobutyric acid (GABA) receptor-like/Cl- channel and a glycine receptor/Cl- channel, have recently been found in mammalian sperm and shown to be involved in the sperm acrosome reaction (AR), an exocytotic event essential to mammalian fertilization. The identity of the receptor/channel involved in the AR depends upon the stimulatory ligand. The AR initiated by the egg zona pellucida requires the participation of the sperm glycine receptor/Cl- channel, whereas the AR initiated by another putative physiological AR initiator, progesterone, involves the sperm GABA receptor-like/Cl- channel. Cl- efflux through the latter has been shown to occur during the progesterone-initiated AR. Activation of sperm amino acid neurotransmitter receptor/Cl- channels may result in plasma membrane depolarization and thereby exert at least a partial control over voltage-sensitive sperm Ca2+ channels and Ca2+ influx important to the AR.

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Neurotransmitter Amino Acids as Modulators of Biological Processes of Spermatozoa

Cited by 5 publications

References 16 publications

Identification of γ-aminobutyric acid transporter (GAT1) on the rat sperm

Identification of γ-aminobutyric acid transporter (GAT1) on the rat sperm

Cellular Localization of GABA and GABA_B Receptor Subunit Proteins During Spermiogenesis in Rat Testis

Amino Acid Neurotransmitter Receptor/Chloride Channels of Mammalian Sperm and the Acrosome Reaction1

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Neurotransmitter Amino Acids as Modulators of Biological Processes of Spermatozoa

Cited by 5 publications

References 16 publications

Identification of γ-aminobutyric acid transporter (GAT1) on the rat sperm

Identification of γ-aminobutyric acid transporter (GAT1) on the rat sperm

Cellular Localization of GABA and GABAB Receptor Subunit Proteins During Spermiogenesis in Rat Testis

Amino Acid Neurotransmitter Receptor/Chloride Channels of Mammalian Sperm and the Acrosome Reaction1

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Cellular Localization of GABA and GABA_B Receptor Subunit Proteins During Spermiogenesis in Rat Testis