Neutralization of early pregnancy factor by passive immunization alters normal embryonic development and cytokine balance

Grosso, M.; Bellingeri, Romina Valeria; Schade, Rüdiger; Vivas, Adriana

doi:10.1016/j.inmuno.2012.09.003

Cited by 2 publications

(1 citation statement)

References 31 publications

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“…Ayrıca, antikor uygulanan sıçanlarda IL-10 ve INF-y seviyeleri yükselirken, serum ve plasental TGF-Beta seviyelerinde önemli bir düşüş izlenmiştir. 10 Tümör proliferasyonu için EGF'nin önemi EGF sadece embriyonun canlılığının devamı için değil, aynı zamanda tümör hücreleri için de gereklidir. EGF'nin tümör hücrelerinin büyümesiyle ilgisi olduğu ilk olarak 1983 yılında Rolfe ve arkadaşları tarafından gösterilmiş-tir.…”

Section: Embriyonik Canlılık Için Egf'nin öNemiunclassified

An immunosupressive protein supporting embryonic implantation and having an important role in cell proliferation: The Early Pregnancy Factor

Özbek¹,

Cengiz²,

Yanar³

2013

Sakarya Med J

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Early pregnancy factor (EPF), identified as an immunosuppresive protein, can be detected in maternal serum within 6-24 hours of fertilization and is present for at least the first two trimester of pregnancy. Besides pregnancy, EPF is secreted by tumor cells, normal growing cells and active platelets during cell growth and division. It is also required for tumor proliferation and tissue renewal. Seventy per cent of the amino acid sequence of EPF derived from human platelets was determined and except from a single residue, it was identical to the sequence of rat mitochondrial chaperonin 10 (cpn 10). The molecular weight of the protein was found to be 10,843 Da. Whether EPF and cpn 10 are the same molecule or not is still discussed by many researchers. The rosette inhibition test (RIT) which is the most widely used and most reliable assay for EPF detection is not practical for routine utilization since it is laborious, time consuming and sensitive. Recently, it is expected to speed up the EPF studies by investigating novel assay systems that will be more practical than RIT.

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Section: Embriyonik Canlılık Için Egf'nin öNemiunclassified

An immunosupressive protein supporting embryonic implantation and having an important role in cell proliferation: The Early Pregnancy Factor

Özbek¹,

Cengiz²,

Yanar³

2013

Sakarya Med J

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Immunohistochemical distribution of early pregnancy factor in ovary, oviduct and placenta of pregnant gilts

Grosso¹,

Bellingeri²,

Motta³

et al. 2014

Biotechnic & Histochemistry

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Early pregnancy factor (EPF) is an immunosuppressant that promotes maternal immune system tolerance of the allogenic fetus. Little is known about localization of this factor in different tissues and nothing has been reported about localization in swine reproductive and placental tissues. We determined the concentration of EPF in serum of gilts and porcine placenta conditioned medium (PPCM). We also analyzed the expression of EPF in different reproductive tissues of pregnant gilts at 10, 30, 60 and 90 days of pregnancy. EPF concentration in serum and PPCM was determined by western blot and densitometry. EPF expression in reproductive tissue was assessed by immunohistochemistry. The highest concentration of EPF was observed at 30 days in serum and PPCM; the concentration was higher in PPCM than in serum at the stages we evaluated. All reproductive tissues from the gestational stages analyzed showed specific labeling of EPF, but this labeling did not appear in non-pregnant gilts. At 30 days pregnancy, the EPF expression in the ovary was predominantly in follicular lutein cells, probably owing to its function as a luteotrophic factor. In the oviduct, EPF was expressed in unciliated secretory epithelial cells and in the cilia of ciliated cells. In the placenta, EPF was expressed in the fetal portion (mesoderm chorioallantois and epithelium of endoderm). EPF acts as an autocrine and paracrine growth factor for the trophoblast during the peri-implantation period.

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Neutralization of early pregnancy factor by passive immunization alters normal embryonic development and cytokine balance

Cited by 2 publications

References 31 publications

An immunosupressive protein supporting embryonic implantation and having an important role in cell proliferation: The Early Pregnancy Factor

An immunosupressive protein supporting embryonic implantation and having an important role in cell proliferation: The Early Pregnancy Factor

Immunohistochemical distribution of early pregnancy factor in ovary, oviduct and placenta of pregnant gilts

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