Neutralizing determinants of canine herpesvirus as defined by monoclonal antibodies

Xuan, Xuenan; Horimoto, Taisuke; Limcumpao, Joselito A.; Takumi, Aki; Tohya, Yukinobu; Takahashi, Eiji; Mikami, Takeshi

doi:10.1007/bf01319241

Cited by 10 publications

(8 citation statements)

References 18 publications

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“…Therefore, it appears that serum neutralizing antibodies can protect puppies against a fatal CHV infection. Three CHV glycoproteins, gp145/112, gp80 and gp47, are known to elicit CHV neutralizing antibodies (Xuan et al, 1991). The genes encoding these glycoproteins have not been identified.…”

Section: Discussionmentioning

confidence: 99%

Nucleotide sequence of the genes encoding the canine herpesvirus gB, gC and gD homologues

Mp²,

Conte³

et al. 1994

Journal of General Virology

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The nucleotide sequence of the genes encoding the canine herpesvirus (CHV) gB, gC and gD homologues was determined. These genes are predicted to encode polypeptides of 879, 459 and 345 amino acids, respectively. Comparison of the predicted amino acid sequences of CHV gB, gC and gD with the homologous sequences from other herpesviruses indicates that CHV is an alphaherpesvirus, a conclusion that is consistent with the previous classification of this virus according to biological properties. Alignment of the homologous gB, gC and gD amino acid sequences indicates that most of the cysteine residues are conserved, suggesting that these glycoproteins possess similar tertiary structures. The nucleotide sequence of the open reading frame downstream from the CHV gC gene was also determined. The predicted amino acid sequence of this putative polypeptide appears to be homologous to a family of proteins encoded downstream from the gC gene in most, although not all, alphaherpesviruses.

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Section: Discussionmentioning

confidence: 99%

Nucleotide sequence of the genes encoding the canine herpesvirus gB, gC and gD homologues

Mp²,

Conte³

et al. 1994

Journal of General Virology

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“…By using monoclonal antibodies (MAbs) against CHV YP11mu strain, three glycoproteins, gB, gC, and gD, were identified [33,35,36]. Almost all MAbs against gB and gC had complement-dependent or complement-enhanced virusneutralizing (VN) activity.…”

Section: Methodsmentioning

confidence: 99%

“…Almost all MAbs against gB and gC had complement-dependent or complement-enhanced virusneutralizing (VN) activity. All of the MAbs against gD possessed complement-independent VN activity [35]. In addition, four of the five MAbs against CHV gD inhibited hemagglutination (HA) activity of CHV against canine red blood cells (RBC) [34].…”

Section: Methodsmentioning

confidence: 99%

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Characterization of Canine Herpesvirus Glycoprotein D (Hemagglutinin).

Maeda

Xuan

Kawaguchi

et al. 1997

The Journal of Veterinary Medical Science

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ABSTRACT. Glycoprotein D (gD) of canine herpesvirus (CHV) YP2 strain was expressed in COS-7 and insect (Spodoptera frugiperda; Sf9) cells. The gDs expressed in COS-7 and Sf9 cells reacted with a panel of monoclonal antibodies (MAbs) against CHV gD (hemagglutinin) and an MAb 25C9 against feline herpesvirus type 1 (FHV-1) gD by indirect immunofluorescence assay, and possessed a molecular weight (MW) of approximately 51-55 and 41-46 kilodalton (kDa), respectively, when examined by immunoblot analysis. After treatment with tunicamycin, the MW of the gD expressed in Sf9 cells became approximately 37 kDa. By hemadsorption (HAD) tests using canine or feline red blood cells (RBC), COS-7 cells expressing CHV gD adsorbed only canine RBC, but not feline RBC, whereas control COS-7 cells expressing FHV-1 gD adsorbed feline RBC, but not canine RBC. By hemagglutination (HA) tests, lysates of Sf9 cells expressing CHV gD agglutinated canine RBC, but not feline RBC. These HA and HAD activities were inhibited by HA-inhibition MAbs against CHV gD. Control lysates of Sf9 cells expressing FHV-1 gD agglutinated only feline RBC. Serum from mice inoculated with lysates of Sf9 cells expressing CHV gD possessed a high titer of virus-neutralizing activities against CHV infection. These results indicated that CHV gD is structurally similar to FHV-1 gD, but is functionally different from FHV-1 gD. -KEY WORDS : canine herpesvirus, feline herpesvirus type 1, glycoprotein D, hemagglutinin.

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“…MAbs: MAbs 11F7, 09D1, 10C10, and 05B7 against CHV gD and an MAb 25C9 against FHV-1 gD were previously produced and characterized [5,12,25,26]. IFA: For detection of CHV gD in IFA, transfected cells were smeared on glass slides, air-dried and then fixed with acetone.…”

Section: Viruses and Cellsmentioning

confidence: 99%

Role of One N-linked Oligosaccharide Chain on Canine Herpesvirus gD in Its Biological Activity.

Maeda

Yokoyama

Fujita

et al. 1997

The Journal of Veterinary Medical Science

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ABSTRACT. The YP11mu strain of a plaque-selected canine herpesvirus (CHV) encoded a smaller molecular weight (MW) of gD than those of other strains including YP2 strain . When nucleotide sequence of the mutated gD of YP11mu strain (gD(YP11mu)) was compared with that of gDs of other CHV strains, gD(YP11mu) lacked 12 nucleotides encoding 4 amino acids, NKTI, including one predicted potential N-linked glycosylation site and no other change was found in other regions. When the gD(YP11mu) and gD of YP2 strain (gD(YP2)) expressed in COS-7 and insect (Spodoptera frugiperda; Sf9) cells were compared each other, both gDs reacted with a panel of monoclonal antibodies (MAbs) against CHV gD by indirect immunofluorescence analysis and the gD (YP11mu) in the virus penetration process remains to be further analyzed.Xuan et al. [25] reported that one plaque-selected CHV, YP11mu strain, possessed a smaller molecular weight (MW) of gD than those of other strains including YP2 strain. However its HA activity and reactivity with antibodies against CHV were similar to those of other strains. Therefore, it seems that mutation in the gD of YP11mu strain does not affect biological activities of the gD. By genetical analysis of this mutation, it is expected to obtain further information on functional region of gD.In this communication, we identified the mutated region on the gD of YP11mu strain and expressed the gD in COS-7 and insect (Spodoptera frugiperda; Sf9) cells. The CHV gD expressed in COS-7 cells specifically adsorbed canine RBC and extracts of CHV gD expressed in Sf9 cells agglutinated canine RBC. Further, antibodies raised in mice immunized with recombinant CHV gDs neutralized CHV infection in vitro. MATERIALS AND METHODS Viruses and cells:Three isolates from our laboratory, YP2, YP11, and the plaque-selected YP11 (YP11mu) [25], two isolates from other laboratories, GCH-1 and Pirene [29], and two reference strains, F-205V and Glasgow CHV2 of CHV were used in this study. All CHV strains were grown in Madin-Darby canine kidney (MDCK) cells for extraction

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Neutralizing determinants of canine herpesvirus as defined by monoclonal antibodies

Cited by 10 publications

References 18 publications

Nucleotide sequence of the genes encoding the canine herpesvirus gB, gC and gD homologues

Nucleotide sequence of the genes encoding the canine herpesvirus gB, gC and gD homologues

Characterization of Canine Herpesvirus Glycoprotein D (Hemagglutinin).

Role of One N-linked Oligosaccharide Chain on Canine Herpesvirus gD in Its Biological Activity.

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