Neutrophil Activation/Maturation Markers in Chronic Heart Failure with Reduced Ejection Fraction

Abstract

Background: Neutrophils are critically involved in the immune response. Inflammatory stimuli alter the expression status of their surface molecule toolset, while inflammation-stimulated granulopoiesis might also influence their maturation status. Data on neutrophil status in heart failure with reduced ejection fraction (HFrEF) are scarce. The present study aims to evaluate the role of neutrophil CD11b, CD66b and CD64 expression in HFrEF. Methods: A total of 135 HFrEF patients and 43 controls were recruited. Mean fluorescence intensity of the activation/maturation markers CD11b, CD66b and CD64 was measured on neutrophils by flow cytometry. CD10 (neprilysin) expression was simultaneously determined. Results: Neutrophil CD64 expression was higher in HFrEF compared with controls, while CD11b/CD66b levels were similar. Neutrophil CD11b and CD66b showed a significant direct correlation to neutrophil CD10 expression (rs = 0.573, p < 0.001 and rs = 0.184, p = 0.033). Neutrophil CD11b and CD66b correlated inversely with heart failure severity reflected by NT-proBNP and NYHA class (NT-proBNP: rs = −0.243, p = 0.005 and rs = −0.250, p = 0.004; NYHA class: p = 0.032 and p = 0.055), whereas no association for CD64 could be found. Outcome analysis did not reveal a significant association between the expression of CD11b, CD66b and CD64 and all-cause mortality (p = ns). Conclusions: The results underline the potential role of neutrophils in HFrEF disease pathophysiology and risk stratification and should stimulate further research, characterizing subpopulations of neutrophils and searching for key molecules involved in the downward spiral of inflammation and heart failure.

Application Value of NT-proBNP Combined with NLR in Evaluation of Major Adverse Cardiac Events in Elderly Patients with Chronic Heart Failure

“…NEU is one of the most abundant leukocyte types and is involved in active nonspecific inflammation. In the process of inflammation, abnormally high activation of NEU leads to the release of multiple proinflammatory factors and proteolytic enzymes, which amplify the inflammatory response and destroy the cardiomyocytes, causing direct damage to the heart function [19,20]. LYM is related to physiological stress and plays an important role in inflammation.…”

Clinical and Immunological Parameters in the Diagnostics of Asthenic Syndrome in Patients with Chronic Heart Failure

“…Субстратами этой протеазы являются, в частности, компоненты сосудистого внеклеточного матрикса -коллаген IV типа и эластин [28]. Принимая во внимание роль ЛЭ в воспалении, можно предположить, что снижение ее активности в крови может определяться функциональной истощенностью нейтрофилов в ходе длительно текущего патологического процесса и/или проникновением части нейтрофилов в первичный очаг повреждения (сердце) вследствие повышения сосудистой проницаемости [29]. Вместе с тем выявленные отрицательные корреляционные связи между активностью ЛЭ и выраженностью астенических расстройств при ХСН, позволяют предположить вовлеченность в патологический процесс также сосудов гематоэнцефалического барьера (ГЭБ) на отдаленных этапах воспаления.…”

Androgen-mediated TGFβ expression suppresses anti-tumor neutrophil response in bone metastatic prostate cancer

“…In support of this, there changes in maturation markers, such as CD10 which was significantly reduced with progression of disease, along with CD66b. Both CD10 and CD66b have been associated with PMN maturity and are highly expressed on fully differentiated PMNs, compared to immature precursors 36, 37 . There was a significant increase in PMNs expressing CD88 (C5aR) in mCRPC compared to mCSPC.…”