Neutrophil and B Cell Expansion in Mice That Lack the Murine IL-8 Receptor Homolog

Cacalano, Grace; Lee, James; Kikly, Kristine; Ryan, Ann Marie; Pitts-Meek, Sharon; Hultgren, Bruce; Wood, William I.; Moore, Mark

doi:10.1126/science.8036519

Cited by 625 publications

(447 citation statements)

References 24 publications

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“…Several studies showed that human PMN produced TNF-α, IL-12 and IL-8 after stimulation with microbial products such as bacterial LPS, Toxoplasma gondii and Candida albicans [10][11][12]. While human PMN normally undergo rapid apoptosis during culture, several inflammatory cytokines (e.g., IL-1β, TNF-α, IL-6, G-CSF, GM-CSF) prolong their life span [4,13].…”

Section: Discussionmentioning

confidence: 99%

Bacterial lipopolysaccharide stimulates bovine neutrophil production of TNF-α, IL-1β, IL-12 and IFN-γ

et al. 2007

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-After intramammary infection, polymorphonuclear neutrophil leukocytes (PMN) are the first cells recruited into the mammary gland. Rapid recruitment of and bacterial phagocytosis and killing by PMN are the most effective defenses against establishment of bacterial infection. In addition to their phagocytic and bactericidal properties, PMN may play a key supportive role through secretion of cytokines during the innate immune response. We sought to determine whether bovine PMN produce cytokines in response to stimulation by lipopolysaccharide (LPS). To investigate the effects of LPS on the expression of cytokines secreted by bovine PMN, we measured the expression of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-12, and interferon (IFN)-γ by ELISA after stimulation with different concentrations of LPS, and secretion of IL-8 after co-stimulation with LPS and either TNF-α or IL-1β. Bovine PMN were shown to secrete TNF-α, IL-1β, IL-12, IL-8 and IFN-γ in response to LPS. Co-incubation of PMN with LPS and TNF-α increased secretion of IL-8 when compared to LPS alone. It was concluded that LPS stimulation up-regulates the secretion of cytokines by bovine PMN, and that co-incubation of LPS with TNF-α had an additive effect on the secretion of IL-8. These data show that bovine PMN, in addition to their phagocytic and bactericidal properties, may play a supportive role in the innate immune response to infection by Gram-negative bacteria through their ability to produce immuno-regulating cytokines.lipopolysaccharide / bovine / neutrophils / cytokines / mastitis

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Section: Discussionmentioning

confidence: 99%

Bacterial lipopolysaccharide stimulates bovine neutrophil production of TNF-α, IL-1β, IL-12 and IFN-γ

et al. 2007

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“…CXCR2ko mice appear outwardly healthy, but previous studies have demonstrated that approximately 25% of these mice exhibited granulopoiesis in the periportal regions of the liver. 39 Although CXCR2ko mice do not normally exhibit hepatic damage, 39 the increased presence of granulocytes in the liver may partly account for the increased susceptibility of these mice to the lethal effects of acetaminophen. However, it should be emphasized that approximately 75% of the CXCRko mice examined in the present study died as a result of acetaminophen challenge.…”

Section: Discussionmentioning

confidence: 99%

Macrophage inflammatory protein-2 gene therapy attenuates adenovirus- and acetaminophen-mediated hepatic injury

et al. 1999

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“…An alternative to knockout a chemokine gene in mice to study the role of the chemokine is to knockout the gene for a chemokine receptor. An example of this is the knockout of the human IL-8R homologue (murine [m]IL-8Rh -/-) in mice in which enhanced neutrophils and B-lymphocyte numbers were apparent [61]. These mIL-8Rh 4-mice had bone marrow cells responsive to inhibition by a number of chemokines including human MIP-la, PF4, P-10 and MCP-1, but they were insensitive to inhibition by human IL-8 1621: These results suggested that IL-8 may be mediating its suppressive effects through CXCR2 (IL-8RB).…”

Section: Inhibition Of Synergistically Stimulated Cell Proliferation mentioning

confidence: 99%

Biology and mechanisms of action of synergistically stimulated myeloid progenitor cell proliferation and suppression by chemokines

1997

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A number of cytokines can act together to stimulate/enhance the proliferation of hematopoietic stem and progenitor cells in a greater than additive fashion. An example of this is the combination of a colony-stimulating factor with a potent costimulating molecule such as steel factor. Certain members of the chemokine family of cytokines can suppress this synergistically enhanced proliferation. This review focuses on cytokines involved in these stimulating/enhancing/suppressing effects with regard to biological activity and what is beginning to be learned about the intracellular signal transduction events that may he mediating these effects. Examples of intracellular mediators involved include, but are not limited to, the Raf-l/ MAP kinase pathway and cyclin-dependent kinase inhibitors p21ci~-l and for cell proliferation, and eukaryotic initiation factor-4E and 4E hinding protein 1 for protein synthesis. Stem Cells 1997; ~~( S U P P~1): [69][70][71][72][73][74][75][76][77][78]

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Neutrophil and B Cell Expansion in Mice That Lack the Murine IL-8 Receptor Homolog

Cited by 625 publications

References 24 publications

Bacterial lipopolysaccharide stimulates bovine neutrophil production of TNF-α, IL-1β, IL-12 and IFN-γ

Bacterial lipopolysaccharide stimulates bovine neutrophil production of TNF-α, IL-1β, IL-12 and IFN-γ

Macrophage inflammatory protein-2 gene therapy attenuates adenovirus- and acetaminophen-mediated hepatic injury

Biology and mechanisms of action of synergistically stimulated myeloid progenitor cell proliferation and suppression by chemokines

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