Neutrophil and monocyte adhesion molecules in bronchopulmonary dysplasia, and effects of corticosteroids

Abstract

Aims: To study a longitudinal change in the expression of adhesion molecules CD11b, CD18, and CD62L on neutrophils and monocytes in very low birth weight babies who develop respiratory distress syndrome, to compare these levels between bronchopulmonary dysplasia (BPD) and non-BPD infants, and to assess the effect of corticosteroid treatment on these adhesion molecules. Methods: Of 40 eligible neonates, 11 neonates were oxygen dependent at 36 weeks (BPD 36 weeks), 16 infants were oxygen dependent at 28 days, but not at 36 weeks (BPD d28), and 13 infants did not develop BPD. Seventeen neonates received a six day course of steroid treatment. Expression of CD11b, CD18, and CD62L was measured on neutrophils and monocytes in arterial blood on days 1, 3, 7, 14, 21, and 28, and before and 2-3 days after initiation of dexamethasone treatment by flow cytometry. Results: CD18 expression on neutrophils and monocytes and CD62L on neutrophils, measured as mean fluorescent intensity, was significantly decreased in BPD neonates compared to non-BPD neonates on days 1-28. Dexamethasone treatment significantly decreased CD11b, CD18, and CD62L expression on neutrophils, and CD11b and CD18L expression on monocytes. Conclusions: Decreased CD18 expression on neutrophils and monocytes, and decreased CD62L expression on neutrophils, measured as mean fluorescent intensity during the first four weeks of life in micropremies may be risk factors and early predictors of BPD. Dexamethasone use was associated with decreased expression of CD11b, CD18, and CD62L.

Protein- and Lipid Modification of Natural Bovine Surfactant

“…On the other hand, prenatally administered corticosteroids with placental permeability mainly exert their action by inducing surfactant synthesis and maturing lung structure with a time interval for optimal response > 48 h after treatment [41]. The supposed mode of action of postnatally administered corticosteroids is postulated by supressing inflammatory activity associated with bronchopulmonary dysplasia and membrane stabilisation [42]. Thus, at least in our setting, the ongoing discussion with respect to short-term effects of corticosteroid treatment in RDS/ ARDS cannot be answered sufficiently.…”

Interleukin-10 Versus Dexamethasone: Effects on Polymorphonuclear Leukocyte Functions of the Newborn

“…However, in the context of the fetal inflammatory response syndrome and its sequelae, our study on circulating PMNs has clinical relevance (2). There are also other PMN functions for example, adhesion, which should be studied; DEX is associated with decreased expression of adhesion molecules on PMNs and monocytes of the newborn (38), but the effect of IL-10 is unknown.…”

Selectin polymorphisms and perinatal morbidity in low‐birthweight infants

“…High E‐selectin levels in cord and peripheral blood [22, 23] have been shown to be predictive of BPD. Low plasma L‐selectin levels during the early postnatal period have been shown to be predictive of length of supplemental oxygen requirement [24] and risk of BPD [25, 26]. Dexamethasone treatment may also affect BPD risk through its impact on L‐selectin expression [27].…”