Neutrophil CD64 expression and serum IL-8: Sensitive early markers of severity and outcome in sepsis

Livaditi, Olga; Κοτανίδου, Αναστασία; Psarra, Aikaterini; Dimopoulou, Ioanna; Sotiropoulou, Christina; Augustatou, Kallirroi; Papasteriades, Chryssa; Armaganidis, Apostolos; Roussos, Charis; Orfanos, Stylianos E.; Douzinas, Emmanuel E.

doi:10.1016/j.cyto.2007.02.007

Cited by 148 publications

(134 citation statements)

References 37 publications

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“…However, it is also increased in cases of SIRS due to non-infectious disease conditions such as severe congestive heart failure or acute pancreatitis, and viral and parasitic infections [30,31]. The accuracy of CD64 index to detect positive sepsis in subjects with VAP was superior compared to other parameters, which was actually in accordance with other studies [32,33]. We also found that in subjects with severe disease, such as VAP-induced sepsis, slightly higher cut-off values for positivity in CD64 index (1.58), CRP (163.5 mg/L), and PCT (1.73 µg/L) levels are required to obtain the best sensitivity, specificity, and predictive values.…”

Section: Discussionsupporting

confidence: 80%

CD64 index on neutrophils can diagnose sepsis and predict 30-day survival in subjects after ventilator-associated pneumonia

Muzlovič

Ihan

Stubljar

2016

J Infect Dev Ctries

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Introduction: Sepsis associated with ventilator-associated pneumonia (VAP) causes mortality in intensive care unit (ICU) patients. The time of diagnosis is crucial, and microbiological cultures take time. In this study, the diagnostic accuracy of CD64 index to predict VAP-induced sepsis and survival time in subjects requiring mechanical ventilation were evaluated and compared to conventional biomarkers and culturing methods. Methodology: A total of 32 subjects with VAP were included. Sepsis after VAP was diagnosed in 25 (78.1%) patients according to clinical signs, radiographic examination, and samples of blood and trachea taken for culturing. Simultaneously with cultures, CD64 index on neutrophils, C-reactive protein (CRP), procalcitonin (PCT), and count of leucocytes and neutrophils were determined. Results: Biomarker values were evaluated in both groups of subjects (with and without sepsis after VAP). The values of CD64 index and CRP were significantly higher in the sepsis group. Receiver operating characteristic (ROC) curve analysis revealed an area under curve (AUC) of 0.929 for CD64 index in differentiating subjects with VAP-induced sepsis from those without sepsis. The biomarkers CRP and PCT showed comparable results (AUC of 0.869 and 0.909, respectively). Blood cultures were positive in 12 subjects, endotracheal aspirate in 19. CD64 index and isolation of pathogen with positive blood cultures or from endotracheal aspirate (positive in 24 cases) could predict survival time before application of more targeted antibiotic therapy. Conclusions: CD64 index may be used as a useful diagnostic tool to recognize VAP-induced sepsis; moreover, accompanied with an identified pathogen, can predict survival for ICU patients.

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Section: Discussionsupporting

confidence: 80%

CD64 index on neutrophils can diagnose sepsis and predict 30-day survival in subjects after ventilator-associated pneumonia

Muzlovič

Ihan

Stubljar

2016

J Infect Dev Ctries

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“…In the present study, serum CRP levels and WBC counts were sensitive and reliable indexes for distinguishing bacterial infection from viral infection. Numerous serum molecules, including IL-6, IL-8, IL-10 and TNF-α, have been reported to be markers of sepsis (5)(6)(7)(9)(10)(11)24,25). Similar to the results of serum CRP level and WBC counts, significantly elevated concentrations of IL-6 and IL-8 were observed in fever patients in the bacterial infection group, regardless of culture-positive or Data presented as the mean ± standard deviation.…”

Section: Discussionsupporting

confidence: 75%

Inflammatory molecules expression pattern for identifying pathogen species in febrile patient serum

Liu

Lin

et al. 2016

Experimental and Therapeutic Medicine

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Abstract.Inflammatory molecules, such as cytokines and chemokines, have been considered markers for bacterial or viral infection in serum of patients in numerous studies. The aim of the present study was to investigate whether we were able to identify the pathogen species through patterns of inflammatory molecules. A total of 132 patients with elevated body temperature (tympanic temperature, >38.3˚C) were recruited for this study. The concentrations of various inflammatory molecules in the patients' serum were evaluated using a cytometric bead array. Higher concentrations of interleukin (IL)-6 and IL-8 were detected in bacterial infection groups (patients with positive and negative blood cultures), as compared with the viral infection group. Viral infection (including influenza and dengue viral infections) was associated with higher concentrations of interferon-γ-inducible protein 10 (IP-10), as compared with the bacterial infection group. In addition, IL-8 levels in the gram-negative bacteria group were higher, as compared with the gram-positive bacteria group. However, IL-8 was insufficient for bacterial species identification. By contrast, dengue virus infection induced the highest serum level of IP-10 among all groups. In conclusion, detection of the patterns of inflammatory molecules may aid the subsequent management and treatment modalities in hospitals, although evaluation of these molecules alone may be insufficient for identifying the pathogen species.

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“…Multiple studies have demonstrated the potential utility of IL-8 in the early diagnosis of systemic bacterial infections, particularly in neonates (17)(18)(19)(20)(21). Of more direct relevance to the current study, previous studies have correlated increased serum IL-8 levels with increased illness severity and mortality in adults and children with septic shock (22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34). To our knowledge, however, the negative predictive value of IL-8 in pediatric septic shock has never been tested in the manner and with the magnitude of the current study.…”

Section: Discussionmentioning

confidence: 64%

Interleukin-8 as a Stratification Tool for Interventional Trials Involving Pediatric Septic Shock

Wong¹,

Cvijanovich²,

Wheeler³

et al. 2008

Am J Respir Crit Care Med

133

103

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Rationale: Interventional clinical trials involving children with septic shock would benefit from an efficient preenrollment stratification strategy. Objectives: To test the predictive value of interleukin (IL)-8 for 28-day mortality in pediatric septic shock. Methods: A training data set (n 5 40) identified a serum IL-8 of greater than 220 pg/ml as having a 75% sensitivity and specificity for predicting 28-day mortality. This cutoff was then subjected to a series of validation steps. Measurements and Main Results: Subjects were drawn from two large, independent pediatric septic shock databases. Prospective application of the IL-8 cutoff to validation data set 1 (n 5 139) demonstrated 78% sensitivity and 64% specificity for 28-day mortality. A serum IL-8 level of 220 pg/ml or less, however, had a negative predictive value for 28-day mortality of 95% in validation data set 1, which was subsequently applied to an independently generated data set of children with septic shock (validation set 2, n 5 193). A serum IL-8 level of 220 pg/ml or less had a negative predictive value for 28-day mortality of 94% when applied to validation set 2. Conclusions: A serum IL-8 level of 220 pg/ml or less, obtained within 24 hours of admission, predicts a high likelihood of survival in children with septic shock. We propose that IL-8 can be used to exclude such patients from interventional clinical trials and ultimately derive a study population with a more favorable risk to benefit ratio when subjected to a study agent.

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Neutrophil CD64 expression and serum IL-8: Sensitive early markers of severity and outcome in sepsis

Cited by 148 publications

References 37 publications

CD64 index on neutrophils can diagnose sepsis and predict 30-day survival in subjects after ventilator-associated pneumonia

CD64 index on neutrophils can diagnose sepsis and predict 30-day survival in subjects after ventilator-associated pneumonia

Inflammatory molecules expression pattern for identifying pathogen species in febrile patient serum

Interleukin-8 as a Stratification Tool for Interventional Trials Involving Pediatric Septic Shock

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