Neutrophil-to-lymphocyte ratio and scoring system for predicting coronary artery lesions of Kawasaki disease

Chang, Ling‐Sai; Lin, Yi-Ju; Yan, Jia-Huei; Guo, Mindy Ming‐Huey; Lo, Mao‐Hung; Kuo, Ho-Chang

doi:10.1186/s12887-020-02285-5

Cited by 45 publications

(50 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our case, we found that these two neutrophil markers are elevated by 58 and 48 folds in KD neutrophil compared to healthy control respectively, which is a strong evidence that neutrophil induced immune disorder existed in KD patient. Neutrophil to leukocyte ratio is a severity marker for KD [26], for further study, we can explore more cases and validate the neutrophil activation pattern stability in KD.…”

Section: Discussionmentioning

confidence: 99%

Utilizing Single-cell RNA Sequencing for Analyzing the Characteristics of PBMC in Patients with Kawasaki Disease

Xue¹,

Zhou

Guo

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: Kawasaki disease (KD) is the main cause of acquired heart disease in children and can lead to coronary artery lesions. This present study was designed to analyze the characteristics of KD peripheral blood mononuclear cells (PBMC) through single-cell RNA sequecing (scRNA-seq) and to explore the potential molecular mechanism of KD.Methods: PBMC was collected from one healthy child and one KD patient, and was used to single-cell RNA sequencing for cell clusters identification and differently expressed gene (DEG) determination. GO function enrichment analysis of DEG in B cell and T cells were performed to explore the most active biological function in KD immune cells. Results: 13 cell clusters can be identified in two samples. Compared with healthy child, naive CD8+ T cell, T helper cell and B cell in KD child were decreased, mainly immune-related T cells, and natural killer T (NKT) cell and neutrophil were increased. Cell activation, lymphocyte activation and regulation of immune system process were 3 GO function shared by all four types of T cells and B cell.Conclusions: Immune cell disorder appears in the KD patient at single cell level by scRNA-seq.

show abstract

Section: Discussionmentioning

confidence: 99%

Utilizing Single-cell RNA Sequencing for Analyzing the Characteristics of PBMC in Patients with Kawasaki Disease

Xue¹,

Zhou

Guo

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…KD as one of the most common autoimmune vasculitis in children also showed abnormally increased neutrophils in varying degrees in the acute stage in our present study. It was widely considered that increased neutrophils played a pivotal role in KD, especially in IVIG resistance (8,9) and CALs formations (14,15), although the precise mechanisms were unknown.…”

Section: Discussionmentioning

confidence: 99%

Neutropenia Is Not Associated With the Outcomes in Patients With Kawasaki Disease

et al. 2021

View full text Add to dashboard Cite

Objective: To investigate the outcomes of coronary artery lesions (CALs) and intravenous immunoglobulin (IVIG) resistance in patients with and without neutropenia during the disease course and to explore the relationships between Δ absolute neutrophils count (ΔANC) and the outcomes.Methods: We retrospectively reviewed the medical records of patients hospitalized in Children's Hospital of Soochow University with a main diagnosis of KD during January 2019 and December 2019. 1:4 propensity score matching was carried out to adjust the baseline characteristics. Smoothed plots and threshold effect analyses were performed to reveal the relationships between ΔANC and the outcomes.Results: Of the 438 patients enrolled, 75 (17.1%) were neutropenia cases and 363 (82.9%) were non-neutropenia cases. Patients with neutropenia were younger, had lower levels of initial ANC, white blood cell (WBC) count and C-reactive protein (CRP). Propensity score matching included 75 neutropenia and 247 non-neutropenia patients. No significant difference was found between neutropenia and non-neutropenia groups regarding CALs, coronary artery aneurysms, irregular coronary lumen, IVIG resistance and days of fever duration. There was a non-linear relationship between ΔANC and IVIG resistance. However, threshold effect analysis showed the incidence of IVIG resistance decreased with increasing ΔANC before the turning point (ΔANC = 1.6) (OR = 0.65, 95% CI = 0.50–0.8.4 P = 0.001). On the other hand, there was a linear relationship between ΔANC and CALs, even after adjusting the confounders (OR = 1.06, 95% CI = 1.02–1.11, P = 0.008).Conclusions: Neutropenia after IVIG was not exactly associated with the outcomes. However, ΔANC was in relation to CALs and IVIG resistance.

show abstract

“…In a systematic meta-analysis review, the neutrophil to lymphocyte ratio (NLR) was shown as an independent risk factor of initial IVIG resistance in KD ( 36 ). However, recent studies have indicated that the predictive value of NLR alone was not good enough to determine IVIG resistance and CAL formation in KD ( 37 , 38 ). We supposed that the neutrophil percentage of peripheral WBCs may play a role in this study.…”

Section: Discussionmentioning

confidence: 99%

The Expression of Glycoprotein Genes in the Inflammatory Process of Kawasaki Disease

Kuo

Yang

et al. 2020

Front. Pediatr.

Self Cite

View full text Add to dashboard Cite

Background: Kawasaki disease (KD) is the most common form of febrile coronary vasculitis disease to occur in children. Early diagnosis and proper therapy can prevent the complication of coronary artery lesions (CAL). The main pathogenesis of KD is an inflammatory process related to the host's genetic characteristics. In innate human immunity, the interaction of leukocytes and glycoprotein plays an important role against microbes. The purpose of our study was to understand the role of leukocytes' glycoprotein genes during the acute phase of KD.Materials and Methods: We enrolled a total of 97 subjects from a medical center. Of those, 24 subjects were healthy controls, and 24 subjects were fever controls; the other 49 subjects were KD patients who had had blood samples taken both before and after IVIG treatment. We collected the total RNA from leukocytes and performed a quantitative polymerase chain reaction for the HP, GRP84, and CLEC4D genes in real time.Results: Compared with both the healthy and fever controls, the upregulation of HP, GRP84, and CLEC4D genes was significant in peripheral leukocytes during acute-phase KD. The transcriptional level of these respective genes not only demonstrated a positive correlation with each other, but were also effective predictors for KD (all auROC >0.87) according to the ROC curve analysis. The hyper-expression of these three genes was significantly associated with IVIG resistance, but not CAL formation.Conclusions: Our study demonstrates that the expression of HP, GRP84, and CLEC4D genes of leukocytes play an important role in the pathogenesis and primary IVIG response during the acute inflammatory process of KD.

show abstract

Neutrophil-to-lymphocyte ratio and scoring system for predicting coronary artery lesions of Kawasaki disease

Cited by 45 publications

References 43 publications

Utilizing Single-cell RNA Sequencing for Analyzing the Characteristics of PBMC in Patients with Kawasaki Disease

Utilizing Single-cell RNA Sequencing for Analyzing the Characteristics of PBMC in Patients with Kawasaki Disease

Neutropenia Is Not Associated With the Outcomes in Patients With Kawasaki Disease

The Expression of Glycoprotein Genes in the Inflammatory Process of Kawasaki Disease

Contact Info

Product

Resources

About