New advances in the mesenchymal stem cells therapy against skin flaps necrosis

Zhang, Fugui

doi:10.4252/wjsc.v6.i4.491

Cited by 11 publications

(10 citation statements)

References 49 publications

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“…Third, ADSCs have superior tolerance to oxidative stress and a better capacity to resist apoptosis than MSCs, and thus, ADSCs may be able to prevent MSC apoptosis to some extent [ 7 ]. Finally, a synergistic effect may exist when MSCs and ADSCs are used together, which maintains the properties of stem cells and increases their osteogenic and angiogenic potential [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Addition of Adipose-Derived Stem Cells to Mesenchymal Stem Cell Sheets Improves Bone Formation at an Ectopic Site

Wang

Dai

et al. 2016

IJMS

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To determine the effect of adipose-derived stem cells (ADSCs) added to bone marrow-derived mesenchymal stem cell (MSC) sheets on bone formation at an ectopic site. We isolated MSCs and ADSCs from the same rabbits. We then prepared MSC sheets for implantation with or without ADSCs subcutaneously in the backs of severe combined immunodeficiency (SCID) mice. We assessed bone formation at eight weeks after implantation by micro-computed tomography and histological analysis. In osteogenic medium, MSCs grew to form multilayer sheets containing many calcium nodules. MSC sheets without ADSCs formed bone-like tissue; although neo-bone and cartilage-like tissues were sparse and unevenly distributed by eight weeks after implantation. In comparison, MSC sheets with ADSCs promoted better bone regeneration as evidenced by the greater density of bone, increased mineral deposition, obvious formation of blood vessels, large number of interconnected ossified trabeculae and woven bone structures, and greater bone volume/total volume within the composite constructs. Our results indicate that although sheets of only MSCs have the potential to form tissue engineered bone at an ectopic site, the addition of ADSCs can significantly increase the osteogenic potential of MSC sheets. Thus, the combination of MSC sheets with ADSCs may be regarded as a promising therapeutic strategy to stimulate bone regeneration.

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Section: Discussionmentioning

confidence: 99%

Addition of Adipose-Derived Stem Cells to Mesenchymal Stem Cell Sheets Improves Bone Formation at an Ectopic Site

Wang

Dai

et al. 2016

IJMS

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“…After excluding 22 irrelevant or reduplicated studies, the remaining 31 studies were further screened. After a screening of the title and abstract of the remaining studies and a careful reading of the full text, 11 studies were excluded for the following reasons: (1) review article [ 22 ], (2) published in Chinese [ 23 , 24 ], (3) inappropriate therapy method [ 25 – 27 ], and (4) no available information [ 28 – 32 ]. Finally, twenty studies were included, and a meta-analysis of stem cells for skin flaps was conducted [ 8 , 18 , 19 , 28 , 33 – 48 ].…”

Section: Resultsmentioning

confidence: 99%

Preclinical efficacy of stem cell therapy for skin flap: a systematic review and meta-analysis

Jiang

Merwe

et al. 2021

Stem Cell Res Ther

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Background A skin flap is one of the most critical surgical techniques for the restoration of cutaneous defects. However, the distal necrosis of the skin flap severely restricts the clinical application of flap surgery. As there is no consensus on the treatment methods to prevent distal necrosis of skin flaps, more effective and feasible interventions to prevent skin flaps from necrosis are urgently needed. Stem therapy as a potential method to improve the survival rate of skin flaps is receiving increasing attention. Methods This review followed the recommendations from the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) statements. Twenty studies with 500 animals were included by searching Web of Science, EMBASE, PubMed, and Cochrane Library databases, up until October 8, 2020. Moreover, the references of the included articles were searched manually to obtain other studies. All analyses were conducted using Review Manager V.5.3 software. Results Meta-analysis of all 20 studies demonstrated stem cell treatment has significant effects on reducing necrosis of skin flap compared with the control group (SMD: 3.20, 95% CI 2.47 to 3.93). Besides, subgroup analysis showed differences in the efficacy of stem cells in improving the survival rate of skin flaps in areas of skin flap, cell type, transplant types, and method of administration of stem cells. The meta-analysis also showed that stem cell treatment had a significant effect on increasing blood vessel density (SMD: 2.96, 95% CI 2.21 to 3.72) and increasing the expression of vascular endothelial growth factor (VEGF, SMD: 4.34, 95% CI 2.48 to 6.1). Conclusions The preclinical evidence of our systematic review indicate that stem cell-based therapy is effective for promoting early angiogenesis by up regulating VEGF and ultimately improving the survival rate of skin flap. In summary, small area skin flap, the administration method of intra-arterial injection, ASCs and MSCs, and xenogenic stem cells from humans showed more effective for the survival of animal skin flaps. In general, stem cell-based therapy may be a promising method to prevent skin flap necrosis.

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“…The application of stem cells has also been proposed to help flap necrosis with promising results . Adipose stromal stem cells have found wide use in many medical disciplines, because they have the advantage of easy isolation and multipotency compared with stem cells of different origins, such as bone marrow stem cells …”

Section: Discussionmentioning

confidence: 99%

Autologous administration of adipose stromal cells improves skin flap survival through neovascularization: An experimental study

Foroglou

Demiri

Koliakos

et al. 2019

International Wound Journal

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One of the most severe complications in aesthetic and reconstructive surgeries is the partial or total necrosis of a skin flap. In our experimental study, we demonstrated the use of adipose‐derived stem cells in the increase of skin flap survival rates. Stem cells were isolated from the fat of Wistar rats and genetically modified to permanently produce a green fluorescent protein (GFP). Two random‐pattern skin flaps (2 cm × 8 cm) were elevated on the dorsal area of the spine, and after being separated from the surgical wounds with a thin silicone sheet, they were placed back onto their original location. Then, the autologous GFP‐producing cells were injected intradermally into the dorsal area of the rats. At the seventh day, after the implantation of the stem cells, a clinical and immunohistochemical control was performed. The fluorescence microscopy revealed green vascular formations, suggesting that autologous GFP stromal cells were converted into endothelial cells through neovascularization. In the control skin flaps, where no stromal cells were used, no fluorescence was observed. The statistical analysis showed significantly lower necrosis rates in the right‐sided flaps (i.e., the flaps where adipose‐derived stromal cells were injected) compared with the left‐sided ones. Findings from our study demonstrate that adipose‐derived stem cells play an important role in the improvement of skin flap survival. Neovascularization is an effective way of achieving it.

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New advances in the mesenchymal stem cells therapy against skin flaps necrosis

Cited by 11 publications

References 49 publications

Addition of Adipose-Derived Stem Cells to Mesenchymal Stem Cell Sheets Improves Bone Formation at an Ectopic Site

Addition of Adipose-Derived Stem Cells to Mesenchymal Stem Cell Sheets Improves Bone Formation at an Ectopic Site

Preclinical efficacy of stem cell therapy for skin flap: a systematic review and meta-analysis

Autologous administration of adipose stromal cells improves skin flap survival through neovascularization: An experimental study

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