New Anti-infective Coatings of Surgical Sutures Based on a Combination of Antiseptics and Fatty Acids

Abstract

Wound infection is a complication feared in surgery. The aim of this study was to develop new anti-infective coatings of surgical sutures and to compare the anti-microbial effectiveness and biocompatibility to the well-established Vicryl Plus. Synthetic absorbable PGA surgical sutures were coated with three different chlorhexidine concentrations and two different octenidine concentrations in combination with palmitic acid and lauric acid. Drug-release kinetics lasting 96 h were studied in phosphate-buffered saline at 37 degrees C. Anti-infective characteristics were determined by measuring the change in optical density of Staphylococcus aureus suspensions charged with coated sutures over time. Microorganisms adsorbed at the surface of coated sutures were assessed on blood agar plates and coated sutures eluted for 24 h were placed on bacterial lawns cultured on Mueller-Hinton plates to prove retained anti-microbial potency. A cell proliferation assay was performed to assess the degree of cytotoxicity. Anti-infective characteristics and biocompatibility were compared to Vicryl Plus. A coating technology for slow-release drug-delivery systems on surgical sutures could be developed. All coatings showed a continuous drug release within 96 h. Individual chlorhexidine and octenidine coated sutures showed superior anti-infective characteristics but inferior biocompatibility in comparison to Vicryl Plus. We conclude that the developed anti-infective suture coatings consisting of lipid-based drug-delivery systems in combination with antiseptics are highly effective against bacterial colonization in vitro; however, drug doses have to be adjusted to improve biocompatibility.

Octenidine Dihydrochloride, a Modern Antiseptic for Skin, Mucous Membranes and Wounds

“…First experiences with coated surgical sutures showed superior efficacy against S. aureus but inferior biocompatibility in comparison to triclosan-coated sutures [44] . Octenidine is only suitable for antimicrobial coatings of tracheotomy tubes to a limited extent unless methods are developed to allow sustained attachment or incorporation to the tubing [67] .…”

“…Recently, a mouth rinse containing octenidine without phenoxyethanol was introduced, which was superior to OPE in terms of taste and local tolerability [44] . This mouth rinse is especially meant for daily mouth hygiene in e.g.…”

Abstracts „Bayern Innovativ'

“…uncemented coated titanium disc: Gentamicinpalmitat und zahlreiche andere Antibiotika-/Antiseptikaverbindungen mit Fettsäuren wurden zwischenzeitlich mit Erfolg zur bakteriziden Ausrüstung von Implantaten wie zementloser Endoprothesen, Wirbelkörper, Zahnimplantate, Gefäßprothesen, Nahtmaterial und Knochenersatzstoffe eingesetzt [9], [7], [4], [5], [3] . In enger Kooperation von Universität und Industrie werden derzeit die Grundlagen für die humane Anwendung erarbeitet.…”

“…[3,[11][12][13]. Diese Erreger sind aber häufig resistent gegenüber einer Vielzahl von Antibiotika [5], Im Gegensatz zu den Pathogenitäts-bzw. Virulenzfaktoren von S. aureus, die gut untersucht sind, ist hierüber bei KNS wenig bekannt.…”

Oftalmik İğne Tipleri ve Sütür Materyalleri

“…21,[34][35][36] Yeni Gelişmeler Oftalmoloji dışındaki bazı disiplinlerde yapılan çalışmalarda triclosan, gümüş katkılı biyoaktif cam, octinidine(palmitik yada laurik asitle kombine), chitosan, tetrasiklin, klorheksidin yada furazolidone gibi antimikrobiyallerle kaplama yapılmış sütürlerin kullanımının enfeksiyon ve sepsis riskini düşürdüğü gözlenmesine rağmen henüz bu sütürler yaygın olarak kullanıma girememiştir. 33,37,38 Günümüzde triclosan içeren polyglactin910 (Resim 4a), poliglecaprone 25 gibi ürünleri ticari olarak elde etmek mümkündür, ancak bunların oftalmik cerrahide güvenle kullanılabilmesini destekleyecek çalışmalar yetersizidir.…”