New antibiotics produced by Bacillus subtilis strains

Маланичева, И. А.; Kozlov, D. G.; Ефименко, Т. А.; Zenkova, Valentina A.; Катруха, Г. С.; Reznikova, M. I.; Королев, А. М.; Borshchevskaya, L. N.; Tarasova, O. D.; Синеокий, С. П.; Efremenkova, Olga V.

doi:10.1134/s0026261714040110

Cited by 5 publications

(4 citation statements)

References 11 publications

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“…For example, Bacillus spp. secrete various antibiotics, such as iturins, fengycins, and mycosubtilins, with different modes of action and different molecular targets (14,17,19,20,49,52). This is consistent with different classes of antimicrobial compounds that have different modes of actions because they interfere with different cellular and physiological processes of the target organisms (47).…”

Section: Discussionmentioning

confidence: 53%

Ex Vivo Application of Secreted Metabolites Produced by Soil-Inhabiting Bacillus spp. Efficiently Controls Foliar Diseases Caused by Alternaria spp

Ali

El-Sayed

Patel

et al. 2016

Appl Environ Microbiol

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Bacterial biological control agents (BCAs) are largely used as live products to control plant pathogens. However, due to variable environmental and ecological factors, live BCAs usually fail to produce desirable results against foliar pathogens. In this study, we investigated the potential of cell-free culture filtrates of 12 different bacterial BCAs isolated from flower beds for controlling foliar diseases caused by Alternaria spp. In vitro studies showed that culture filtrates from two isolates belonging to Bacillus subtilis and Bacillus amyloliquefaciens displayed strong efficacy and potencies against Alternaria spp. The antimicrobial activity of the culture filtrate of these two biological control agents was effective over a wider range of pH (3.0 to 9.0) and was not affected by autoclaving or proteolysis. Comparative liquid chromatography-mass spectrometry (LC-MS) analyses showed that a complex mixture of cyclic lipopeptides, primarily of the fengycin A and fengycin B families, was significantly higher in these two BCAs than inactive Bacillus spp. Interaction studies with mixtures of culture filtrates of these two species revealed additive activity, suggesting that they produce similar products, which was confirmed by LC-tandem MS analyses. In in planta pre-and postinoculation trials, foliar application of culture filtrates of B. subtilis reduced lesion sizes and lesion frequencies caused by Alternaria alternata by 68 to 81%. Taken together, our studies suggest that instead of live bacteria, culture filtrates of B. subtilis and B. amyloliquefaciens can be applied either individually or in combination for controlling foliar diseases caused by Alternaria species. Many species in the genus Alternaria cause significant yield and quality losses in food and ornamental crops (1). These species primarily attack aerial plant parts, including foliage, fruits, and stems, and cause a variety of symptoms ranging from necrotic leaf spots to enlarged blighted shoots, eventually resulting in defoliation and loss in yield and quality. Alternaria diseases could be managed with a combination of cultural practices, fungicides, and, if available, genetic resistance. Since many crop cultivars lack sufficient resistance to Alternaria spp., fungicide sprays are the primary means of controlling Alternaria diseases (1). However, loss in the efficacy of several commonly used fungicides for controlling Alternaria spp. has been reported (2-6). Loss of effective fungicides requires discovery and development of new and safe alternative chemicals. Although fungicides with newer chemistry are promising, their introduction into crop production systems is slow, and resistant field isolates of the pathogen usually appear very quickly, within a few seasons of the introduction of a new fungicide (5, 7, 8). As with many other plant pathogens, in most cases fungicide resistance in Alternaria spp. has been attributed to single-site mutations in the target genes (see, e.g., references 9, 10, and 11). Therefore, finding multisite-acting fungicides t...

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Section: Discussionmentioning

confidence: 53%

Ex Vivo Application of Secreted Metabolites Produced by Soil-Inhabiting Bacillus spp. Efficiently Controls Foliar Diseases Caused by Alternaria spp

Ali

El-Sayed

Patel

et al. 2016

Appl Environ Microbiol

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“…However, the new generation of antibiotics should be effective against drugresistant pathogens such as methicillin-resistant Staphylococcus aureus (MRSA), vancomycinresistant E. coli, Pseudomonas aeruginosa, or Acinetobacter baumannii that are very serious causative agents in nosocomial infections and treat public health. Nevertheless, what is essential to control these resistant strains relates to researchers' attempt to exploit such abilities (broad-spectrum, fast killing, and high efficacy) among Bacillus-produced AMPs by using cutting-edge approaches, isolating new strains from unusual environments or making modified types of them using bioengineering [106]. For therapeutic applications in humans, it should not be neglected to study their mechanism of action, immunogenicity, and toxicity before clinical trials.…”

Section: Pharmaceutical Industrymentioning

confidence: 99%

Bacillales: From Taxonomy to Biotechnological and Industrial Perspectives

et al. 2022

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For a long time, the genus Bacillus has been known and considered among the most applicable genera in several fields. Recent taxonomical developments resulted in the identification of more species in Bacillus-related genera, particularly in the order Bacillales (earlier heterotypic synonym: Caryophanales), with potential application for biotechnological and industrial purposes such as biofuels, bioactive agents, biopolymers, and enzymes. Therefore, a thorough understanding of the taxonomy, growth requirements and physiology, genomics, and metabolic pathways in the highly diverse bacterial order, Bacillales, will facilitate a more robust designing and sustainable production of strain lines relevant to a circular economy. This paper is focused principally on less-known genera and their potential in the order Bacillales for promising applications in the industry and addresses the taxonomical complexities of this order. Moreover, it emphasizes the biotechnological usage of some engineered strains of the order Bacillales. The elucidation of novel taxa, their metabolic pathways, and growth conditions would make it possible to drive industrial processes toward an upgraded functionality based on the microbial nature.

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“…Thuricin 7 is the new bacteriocin produced by Bacillus thuringiensis BMG1.7 (Cherif et al 2001). Two unidentified nonproteinaceous amino acids produced by the Bacillus subtilis strain of INA 01085 are also potential as new antibiotics (Malanicheva et al 2014). Bacillus atrophaeus also produces new compounds that are different from bacitracin (Ebrahimipour et al 2014).…”

Section: Csamentioning

confidence: 99%

Antimicrobial activities and phylogenetic study of bacteria associated with Cyperus rotundus rhizosphere from Cemoro Sewu Plateau, Indonesia

et al. 2019

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Abstract. Ambarwati A, Wahyuono S, Moeljopawiro S, Sembiring L, Yuwono T. 2019. Antimicrobial activities and phylogenetic study of bacteria associated with Cyperus rotundus rhizosphere from Cemoro Sewu Plateau, Indonesia. Biodiversitas 20: 2206-2212. A study has been conducted to investigate the activity of bacteria isolated from the rhizosphere of Purple Nut Sedge (Cyperus rotundus) from Cemoro Sewu Plateu, Indonesia as antimicrobial agent and the molecular relatedness between the isolates with other bacteria based on phylogenetic tree analysis. A total of six bacteria were obtained and tested for their capability to inhibit the growth of test organisms using agar block method and characterized molecularly using 16S rDNA sequence analysis. It was observed that the six isolates demonstrated the ability to inhibit at least one of four test microorganisms growth, with a diameter of inhibition zone ranging from 8-35 mm. The CRC32 isolate was the best isolate in inhibiting Staphylococcus aureus ATCC 25923 with a diameter inhibition zone of 26 mm (strong), while isolate CRA8 was the best anti-Candida (showing 35 mm of inhibition zone). The phylogenetic tree analysis resulted in two genera, four isolates belonging to Bacillus, while the other two isolates were known related to Paenibacillus genus. BLAST analysis on the CRC 32 isolate revealed that it is closely related with Paenibacillus peroriae strain 3763 with 99% sequence similarities and CRA8 isolates demonstrated the highest similarity with Paenibacillus sonchi strain X19-5 (97%). The results of this study demonstrated that the bacterial isolates from plant rhizosphere of Cemoro Sewu Plateau were the members of the Bacillus and Paenibacillus genera and demonstrated the potential as antimicrobial agent.

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New antibiotics produced by Bacillus subtilis strains

Cited by 5 publications

References 11 publications

Ex Vivo Application of Secreted Metabolites Produced by Soil-Inhabiting Bacillus spp. Efficiently Controls Foliar Diseases Caused by Alternaria spp

Ex Vivo Application of Secreted Metabolites Produced by Soil-Inhabiting Bacillus spp. Efficiently Controls Foliar Diseases Caused by Alternaria spp

Bacillales: From Taxonomy to Biotechnological and Industrial Perspectives

Antimicrobial activities and phylogenetic study of bacteria associated with Cyperus rotundus rhizosphere from Cemoro Sewu Plateau, Indonesia

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