New approach to improve degradation of recalcitrant azo dyes by Streptomyces spp. and Phanerochaete chrysosporium

Paszczyński, Andrzej; Pasti, Maria B.; Goszczyński, Stefan; Crawford, Don L.; Crawford, Ronald L.

doi:10.1016/0141-0229(91)90198-j

Cited by 117 publications

(44 citation statements)

References 31 publications

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“…The precipitate contained azobenzene-4,4'-disulfonic acid. Its dimethyl ester, characterized by mass spectrum m/z (relative intensity [percent]) 372 (4), 371 (6), 370 (M+, 30), 199 (35), 185 (40), 171 (100), 156 (33), was one of the components of the reaction mixture (Fig. 3).…”

Section: Resultsmentioning

confidence: 99%

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New pathway for degradation of sulfonated azo dyes by microbial peroxidases of Phanerochaete chrysosporium and Streptomyces chromofuscus

et al. 1994

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Pathways for the degradation of 3,5-dimethyl-4-hydroxy-azobenzene-4'-sulfonic acid (I) and 3-methoxy-4-hydroxyazobenzene-4'-sulfonamide (II) by the manganese peroxidase and ligninase of Phanerochaete chrysosporium and by the peroxidase of Streptomyces chromofuscus have been proposed. Twelve metabolic products were found, and their mechanisms of formation were explained. Preliminary oxidative activation of the dyes resulted in the formation of cationic species, making the molecules vulnerable to the nucleophilic attack of water. Two types of hydrolytic cleavage were observed. Asymmetric splitting gave rise to quinone and diazene derivatives, while symmetric splitting resulted in the formation of quinone monoimine and nitroso derivatives. These unstable intermediates underwent further redox, oxidation, and hydrolytic transformation, eventually furnishing 11 organic products and ammonia.

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Section: Resultsmentioning

confidence: 99%

“…also degraded the modified dye. Acid Yellow 9 was not recognized by these bacteria as a substrate (35). Decolorization (2,42,45).…”

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New pathway for degradation of sulfonated azo dyes by microbial peroxidases of Phanerochaete chrysosporium and Streptomyces chromofuscus

et al. 1994

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“…Este fungo tem a capacidade de mineralizar, além da lignina, pelo menos parcialmente e em alguns casos completamente, uma variedade de poluentes resistentes a degradação [18][19][20][21] . O sistema lignolítico deste fungo é representado principalmente pelas enzimas lignina e manganês peroxidase, as quais são produzidas em meios contendo fontes limitadas de carbono e nitrogênio.…”

Section: úTlimos Avanços No Tratamento De Corantes E Efluentes Têxteiunclassified

Novas tendências no tratamento de efluentes têxteis

Kunz¹,

Peralta‐Zamora²,

Moraes³

et al. 2002

Quím. Nova

340

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Recebido em 25/7/00; aceito em 6/6/01 NEW TENDENCIES ON TEXTILE EFFLUENT TREATMENT. Textile effluents, when not correctly treated, cause a high impact to the environment. The main recalcitrant compounds present in textile effluent are represented by the synthetic dyes, used during the fibber dying process. Among others, the azo dyes are considered the most harmful due to its mutagenic and carcinogenic character. In the present work we reported a revision study on the new tendencies for remediation of textile effluents, mainly to degrade the recalcitrant compounds. For this purpose, chemical, physical, photochemical, biological and combined processes were investigated.Keywords: textile effluents; treatment processes; recalcitrant compounds. INTRODUÇÃONas últimas décadas, os problemas ambientais têm se tornado cada vez mais críticos e freqüentes, principalmente devido ao desmedido crescimento populacional e ao aumento da atividade industrial. Com estes ingredientes os problemas devido a ação antrópica têm atingido dimensões catastróficas, podendo ser observadas através de alterações na qualidade do solo, ar e água.Sem dúvida, a contaminação de águas naturais tem sido um dos grandes problemas da sociedade moderna. A economia de água em processos produtivos vem ganhando especial atenção devido ao valor agregado que tem sido atribuído a este bem, através de princípios como consumidor pagador e poluidor pagador recentemente incorporados em nossa legislação. Aliado a isso, temos previsões não muito animadoras para o século que se inicia, como por exemplo a previsão feita pela Companhia de Saneamento do estado de São Paulo (SABESP), que estima que já em 2010 a demanda de água será superior a capacidade hídrica dos mananciais do estado 1 . Dentro deste contexto, o setor têxtil apresenta um especial destaque, devido a seu grande parque industrial instalado gerar grandes volumes de efluentes, os quais, quando não corretamente tratados, podem causar sérios problemas de contaminação ambiental.Os efluentes têxteis caracterizam-se por serem altamente coloridos, devido à presença de corantes que não se fixam na fibra durante o processo de tingimento 2 . A importância dos corantes para a civilização humana é evidente e bem documentada 3 . Os corantes sintéticos são extensivamente utilizados na indústria têxtil, gráfica, fotográfica e como aditivos em derivados de petróleo. Aproximadamente 10.000 diferentes corantes e pigmentos são usados industrialmente, o que representa um consumo anual de cerca de 7 x 10 5 tons no mundo 4-6 e 26.500 tons somente no Brasil 7 . A molécula do corante utilizada para tingimento da fibra têxtil pode ser dividida em duas partes principais, o grupo cromóforo e a estrutura responsável pela fixação à fibra.Existem vários grupos cromóforos utilizados atualmente na sín-tese de corantes. No entanto, o grupo mais representativo e largamente empregado pertence a família dos azocorantes (Figura 1), que se caracterizam por apresentarem um ou mais grupamentos -N=N-ligados a sistemas aromáticos. Os azocorantes r...

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“…In particular, the white-rot fungus Phanerochaete chrysosporium was extensively studied as a dye-decolorizing fungus (5,9,19,20,21,30) and several lignin peroxidases (LiPs) of P. chrysosporium have been reported to show decolorizing activity. However, their decolorizing spectrum toward dyes is not extensively investigated, mainly because research on their lignin-degrading reaction is focused.…”

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Efficient Heterologous Expression in Aspergillus oryzae of a Unique Dye-Decolorizing Peroxidase, DyP, of Geotrichum candidum Dec 1

Sugano

Nakano

Sasaki

et al. 2000

Appl Environ Microbiol

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Efficient expression of the dye-decolorizing peroxidase, DyP, from Geotrichum candidum Dec 1 in Aspergillus oryzae M-2-3 was achieved by fusing mature cDNA encoding dyp with the A. oryzae ␣-amylase promoter (amyB). The activity yield of the purified recombinant DyP (rDyP) was 42-fold compared with that of the purified native DyP from Dec 1. No exogenous heme was necessary for the expression of rDyP in A. oryzae. From the N-terminal amino acid sequence analyses of native DyP and rDyP, the absence of a histidine residue in both DyPs, which was considered to be important for heme binding of DyP, was confirmed. These results suggest that rDyP without a typical heme-binding region produced by A. oryzae exhibits a function similar to that of native DyP.The newly isolated Geotrichum candidum Dec 1 was found to decolorize 21 kinds of synthetic dyes (13), and its degradation spectrum in relation to synthetic dyes is wider than that of any other decolorizing organisms reported so far. In our previous study, an extracellular enzyme, DyP (for dye-decolorizing peroxidase), was found to be responsible for the decolorization of dyes. DyP degraded phenolic compounds, such as 2,6-dimethoxyphenol and guaiacol, while it did not degrade nonphenolic veratryl alcohol (14). Considering its substrate specificity and molecular mass, DyP was found to be a novel peroxidase distinct from other peroxidases reported previously (25,26,28,41). Furthermore, the absorption spectrum of DyP exhibited a Soret band at 406 nm corresponding to a hemoprotein, and its Na 2 S 2 O 4 -reduced form revealed a peak at 556 nm that indicates the presence of a protoheme as its prosthetic group (14). We also reported the cloning of cDNA of the dyp gene (32).So far, several microorganisms capable of decolorizing some synthetic dyes have been reported (17-20, 22, 23). In particular, the white-rot fungus Phanerochaete chrysosporium was extensively studied as a dye-decolorizing fungus (5,9,19,20,21,30) and several lignin peroxidases (LiPs) of P. chrysosporium have been reported to show decolorizing activity. However, their decolorizing spectrum toward dyes is not extensively investigated, mainly because research on their lignin-degrading reaction is focused. Furthermore, although cloning and expression of several LiPs have been reported (6,11,29), there is no report on the enhancement of the yield of these enzymes by heterologous expression.Therefore, we focused on producing a large amount of DyP having dye-decolorizing activity in Aspergillus oryzae under the control of the amyB promoter. A. oryzae is known to exhibit a high growth rate and to be a safe host (1); furthermore, it can secrete gram-per-liter quantities of heterologous protein (4). From this study and our previous data, we show that DyP is a unique peroxidase.Chemicals, enzymes, and other materials. Ten kinds of synthetic dyes kindly provided by Nippon Kayaku Co., Ltd. (Tokyo, Japan), and Bayer Japan Co., Ltd. (Tokyo, Japan), were used. Cellulase and lysing enzymes were obtained from SigmaAldrich Japan...

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New approach to improve degradation of recalcitrant azo dyes by Streptomyces spp. and Phanerochaete chrysosporium

Cited by 117 publications

References 31 publications

New pathway for degradation of sulfonated azo dyes by microbial peroxidases of Phanerochaete chrysosporium and Streptomyces chromofuscus

New pathway for degradation of sulfonated azo dyes by microbial peroxidases of Phanerochaete chrysosporium and Streptomyces chromofuscus

Novas tendências no tratamento de efluentes têxteis

Efficient Heterologous Expression in Aspergillus oryzae of a Unique Dye-Decolorizing Peroxidase, DyP, of Geotrichum candidum Dec 1

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