New pathway for degradation of sulfonated azo dyes by microbial peroxidases of Phanerochaete chrysosporium and Streptomyces chromofuscus

Goszczyński, Stefan; Paszczyński, Andrzej; Pasti-Grigsby, M B; Crawford, Ronald L.; Crawford, Don L.

doi:10.1128/jb.176.5.1339-1347.1994

Cited by 194 publications

(103 citation statements)

References 41 publications

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“…The chemical structure of the dye could be the reason for extending the time for the decolorization. The degradability of acid red G was better than RBR X-3B, possibly due to its lower molecular mass and also attributed due to the structural differences [13]. These results suggest the efficiency of A. calcoaceticus has a potential to degrade textile dyes containing different chromophore groups.…”

Section: Comparison Of Decolorization Capabilities Of a Calcoaceticumentioning

confidence: 86%

Decolorization of Textile Dyes and Degradation of Mono-Azo Dye Amaranth by Acinetobacter calcoaceticus NCIM 2890

et al. 2011

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Acinetobacter calcoaceticus NCIM 2890 (A. caloaceticus) was found to decolorize 20 different textile dyes of various classes. Decolorization of an azo dye amaranth was observed effectively (91%) at static anoxic condition, whereas agitated culture grew well but showed less decolorization (68%) within 48 h of incubation. Induction of intracellular and extracellular lignin peroxidase, intracellular laccase, dichlorophenol indophenol (DCIP) reductase and riboflavin reductase represented their involvement in the biodegradation of amaranth. The products obtained after degradation of Amaranth were characterized as naphthalene sulfamide, hydroxyl naphthalene diazonium and naphthalene diazonium. The germination and growth of Sorghum vulgare and Phaseolus mungo seeds, and the growth of E. coli and Bacillus substilis were not inhibited by the metabolic products of the dye.

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Section: Comparison Of Decolorization Capabilities Of a Calcoaceticumentioning

confidence: 86%

Decolorization of Textile Dyes and Degradation of Mono-Azo Dye Amaranth by Acinetobacter calcoaceticus NCIM 2890

et al. 2011

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“…Biodegradation of widely used synthetic dyes in textile industry especially azo ones by P. chrysosporium has been investigated previously (Cripps et al, 1990;Goszczynski et al, 1994;Beydilli et al, 1998;Naidu et al, 2003). The lignin degrading system of white-rot fungi is consisted of various extracellular enzymes such as laccases, peroxidases and oxidases (Shah and Nerud, 2002).…”

Section: Resultsmentioning

confidence: 99%

Decolorization of different azo dyes by Phanerochaete chrysosporium RP78 under optimal condition

Ghasemi

Tabandeh

Bambai

et al. 2010

Int. J. Environ. Sci. Technol.

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ABSTRACT:Detoxification of synthetic dyes is one of the main challenges in clearing textile industry wastes.Biodegradation of azo-dyes using Phanerochaete chrysosporium is one the most environmentally friendly methods available. The main enzymes responsible for mycodecolorization process are lignin and manganese peroxidases. Here, optimization of expression conditions has been carried out with manipulating culture condition and nutrient sources. Therefore, the effects of buffer and temperature as well as nitrogen source on lignin peroxidase and manganese peroxidase production were investigated at two levels and four levels, respectively. For this purpose, P. chrysosporium RP78 based on Taguchi design of experiment has been applied. Maximum lignin and manganese peroxidase activities of 182 ± 2.5 U/L and 850 ± 41 U/L were obtained under predicted optimum conditions, respectively. Thereby, about 100 % decolorization was achieved after 24 h for two most widely used groups of azo dyes in textile industry consisting reactive and acidic. The physical adsorption of the azo dyes by mycelia was not significant which indicated that the enzymatic degradation of the dyes was occurred. Time profile of these enzymes showed that manganese peroxidase was peaked on 9 th day while lignin peroxidase peaked on 13 th. day and remained stable in the culture. The extracellular expression profiles of both were studied by 2 dimensional gel electrophoresis to partially characterize the enzymes.

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“…While fungal treatment of effluents is usually very time-consuming (3), immobilized enzymes could have potential for dye decolorization and recycling of effluents (1) without the need for the addition of growth substrates. The enzymes involved in the degradation of azo dyes are mainly peroxidases (12) and laccases (1). However, a significant cost reduction for these enzymes would be required in order to make this process economically more attractive.…”

mentioning

confidence: 99%

A New Alkali-Thermostable Azoreductase from Bacillus sp. Strain SF

Maier

Kandelbauer

Erlacher

et al. 2004

Appl Environ Microbiol

217

100

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A screening for dye-decolorizing alkali-thermophilic microorganisms resulted in a Bacillus sp. strain isolated out of the wastewater drain of a textile finishing company. An NADH-dependent azoreductase of this strain, Bacillus sp. strain SF, was found to be responsible for the decolorization of azo dyes. This enzyme was purified by a combination of ammonium sulfate precipitation and anion-exchange and affinity chromatography and had a molecular mass of 61.6 kDa and an isoelectric point at pH 5.3. The pH optimum of the azoreductase depended on the substrate and was within the range of pHs 8 to 9, while the temperature maximum was reached at 80°C. Decolorization only took place in the absence of oxygen and was enhanced by FAD, which was not consumed during the reaction. A 26% similarity of this azoreductase to chaperonin Cpn60 from a Bacillus sp. was found by peptide mass mapping experiments. Substrate specificities of the azoreductase were studied by using synthesized model substrates based on di-sodium-(R)-benzyl-azo-2,7-dihydroxy-3,6-disulfonyl-naphthaline. Those dyes with NO 2 substituents, especially in the ortho position, were degraded fastest, while analogues with a methyl substitution showed the lowest degradation rates.In the last few years, environmental legislation, e.g., about the appearance of color in discharges, combined with the increasing cost of water for the industrial sector, has made the treatment and reuse of dyeing effluents increasingly attractive to the industry. The common method for the treatment of wastewater in the textile finishing industry is physicochemical flocculation in combination with biological treatment (17). The conventional treatment of colored effluents produces a lot of sludge, but does not remove all dyes, thus preventing recycling of the treated wastewater. In activated sludge treatments, dyeing effluents, e.g., reactive azo dyes and naphthaline-sulfonic acids as well as aromatic amino derivatives, represent an extensive nonbiodegradable class of compounds (18) and can even inhibit activated sludge organisms. Thus, the development of more effective biological systems for the treatment of these types of wastewater has resulted in considerable interest by the industry.Wood-rotting fungi (e.g., Phanerochaete chrysosporium, Trametes sp., and Aspergillus sp.) have been found to effectively degrade a variety of azo dyes under aerobic conditions (16). Dye-degrading fungi have been used frequently in bioreactors for the decolorization and degradation of azo dyes (37). While fungal treatment of effluents is usually very time-consuming (3), immobilized enzymes could have potential for dye decolorization and recycling of effluents (1) without the need for the addition of growth substrates. The enzymes involved in the degradation of azo dyes are mainly peroxidases (12) and laccases (1). However, a significant cost reduction for these enzymes would be required in order to make this process economically more attractive. Aerobic bacteria have been described to oxidatively decolorize m...

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New pathway for degradation of sulfonated azo dyes by microbial peroxidases of Phanerochaete chrysosporium and Streptomyces chromofuscus

Cited by 194 publications

References 41 publications

Decolorization of Textile Dyes and Degradation of Mono-Azo Dye Amaranth by Acinetobacter calcoaceticus NCIM 2890

Decolorization of Textile Dyes and Degradation of Mono-Azo Dye Amaranth by Acinetobacter calcoaceticus NCIM 2890

Decolorization of different azo dyes by Phanerochaete chrysosporium RP78 under optimal condition

A New Alkali-Thermostable Azoreductase from Bacillus sp. Strain SF

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