Decolorization of different azo dyes by Phanerochaete chrysosporium RP78 under optimal condition

Abstract

ABSTRACT:Detoxification of synthetic dyes is one of the main challenges in clearing textile industry wastes.Biodegradation of azo-dyes using Phanerochaete chrysosporium is one the most environmentally friendly methods available. The main enzymes responsible for mycodecolorization process are lignin and manganese peroxidases. Here, optimization of expression conditions has been carried out with manipulating culture condition and nutrient sources. Therefore, the effects of buffer and temperature as well as nitrogen source on lignin peroxidase and manganese peroxidase production were investigated at two levels and four levels, respectively. For this purpose, P. chrysosporium RP78 based on Taguchi design of experiment has been applied. Maximum lignin and manganese peroxidase activities of 182 ± 2.5 U/L and 850 ± 41 U/L were obtained under predicted optimum conditions, respectively. Thereby, about 100 % decolorization was achieved after 24 h for two most widely used groups of azo dyes in textile industry consisting reactive and acidic. The physical adsorption of the azo dyes by mycelia was not significant which indicated that the enzymatic degradation of the dyes was occurred. Time profile of these enzymes showed that manganese peroxidase was peaked on 9 th day while lignin peroxidase peaked on 13 th. day and remained stable in the culture. The extracellular expression profiles of both were studied by 2 dimensional gel electrophoresis to partially characterize the enzymes.

Dye Waste Treatment

“…By using response surface OPEN ACCESS methodology, the optimal conditions for chromate reduction and dye decolorization were found to be 0.24 g/L glucose, 3.0 g/L (NH 4 ) 2 SO 4 and 0.2 g/L peptone at pH 7 and 35 °C. Ghasemi and colleagues (2010) studied the decolorization of different azo dyes by Phanerochaete chrysosporium RP 78 under optimized conditions [2]. Experimental results showed that the decolorization of azo dyes was due to an enzymatic degradation and 100% decolorization was achieved after 24 hours of reaction.…”

Decolorization of Anthraquinonic Dyes from Textile Effluent Using Horseradish Peroxidase: Optimization and Kinetic Study

“…Enzymes can act on specific recalcitrant pollutants to remove them by precipitation or transformation to other products. Amongst oxidoreductive enzymes that are involved in dye decolorization, peroxidases such as horseradish peroxidase (HRP), citochrome C peroxidase isolated from yeast, chloroperoxidase isolated from Caldariomyces fumago , several fungal lignin peroxidase (LiP) and manganese peroxidase (MnP) such as LiP from Phanerochaete chrysosporium , and soybean peroxidase have been reported as excellent oxidant agents to degrade dyes in the presence of hydrogen peroxide [ 17 – 21 ].…”

Biodecolorization of Reactive Yellow‐2 by Serratia sp. RN34 Isolated from Textile Wastewater

“…In recent years, use of biological agents has attracted worldwide attention for the removal of azo-dyes because of being relatively cheaper and environment friendly in nature (Verma and Madamwar, 2003). Investigated agents include bacteria (Hussain et al, 2013;Mahmood et al, 2013;Anwar et al, 2014); algae (Daneshvar et al, 2007); fungi (Casieri et al, 2008;Ghasemi et al, 2010); and actinomycetes (Khehra et al, 2005). Several researchers have reported the potential of various bacterial strains for removal of azo-dyes (Kalme et al, 2007;Hong et al, 2007;Khalid et al, 2012;Hussain et al, 2013;Anwar et al, 2014).…”