New Approaches for Diagnosis of Viral Diseases in Animals

Minakshi, P.

doi:10.14737/journal.aavs/2014/2.4s.55.63

Cited by 7 publications

(7 citation statements)

References 18 publications

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“…It is worldwide spread disease was firstly described in 1946 as an extremely infectious disease occur as enteric forms in the swine (Weiss and Navas-Martin,2005). Virus replication and targets the absorpative epithelial cells of villi and cause villous atrophy and severe necrosis to enterocyte of ileum and jejunum during twenty four hour after exposure to pathogen lead to disturbances in the enzyme activity, digestion and imbalance electrolytes led to accumulated the fluid in the lumen of the intestine , severe malabsorptive diarrhea (Moon et al,1975 andWu et al,2020) severe TGVE are more common in small aged piglets and death uncommon after aged 2-3 weeks, the disease symptoms involved copious yellow watery diarrhea, vomiting, dryness and fast loss of body weight, vomiting animals was unusual but main clinical signs is the watery diarrhea, , the upper respiratory tract may often affect(Enjuanes et al, 1995).…”

Section: Transmissible Gastroenteritis (Tgve)mentioning

confidence: 99%

A Focus On Coronaviruses Infections In Animals: Review Article

Taee

Ismail

Al-Saidya

et al. 2020

Journal of Applied Veterinary Sciences

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Coronaviruses are large, enveloped and single-stranded, non-segmented with positive sense RNA virus belong to Nidovirus, family Coronavirirdae and included two subfamilies Orthocoronavirinae and Letovirinae. There are four genera variable in antigenestic characteristic and cross-reactivity (Alpha-coronavirus, Beta-coronavirus, Gamma-coronavirus and Deltacoronavirus) affect mammals and birds while Bafinivirus affect fish and cause different diseases in the vertebrate animals and human, so this review listed most important diseases in some animals which cause economic problems as in the avian cause infectious bronchitis(IB), in the bovine cause diseases in both bowl and respiratory system, Enteric form Disease in the equine, two forms of diseases have been occurred in the feline (feline enteric coronavirus and feline infectious peritonitis), in canine cause enteric and respiratory syndroms while in the porcine the diseases occur as are Transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhoea virus (PEDV), and porcine deltacoronavirus (PDCoV).Also corona viruses affect aquatic animals and cause general clinical signs in fish. Some of these pathogens transmitted from camel and bates and causes diseases for human as SARS, MERS and SARS-2.From these review the conclusion is the hygiene practices and biosecurity are more important to prevent the emerging diseases in both human and animals cause by coronavirus.

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Section: Transmissible Gastroenteritis (Tgve)mentioning

confidence: 99%

A Focus On Coronaviruses Infections In Animals: Review Article

Taee

Ismail

Al-Saidya

et al. 2020

Journal of Applied Veterinary Sciences

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“…The output of SSPACE was used in PROkaryotic DynamIc programming Gene finding ALgorithm (PRODIGAL) to identify codon DNA sequences CDSs (Minakshi et al 2014). The genes prediction was carried out with the command:…”

Section: Bacterial Gene Prediction and Annotationmentioning

confidence: 99%

“…Reads length varies between 50 and 250 bp and up to 3 billion reads as the output. The main advantage of Illumina technology is the high sequence yield while the two major disadvantages of this technology is the need of high DNA concentration and the high cost (Minakshi et al 2014). The NGS-data analysis may be a dilemma if the wrong analysis strategy is followed.…”

Section: Introductionmentioning

confidence: 99%

Next Generation Sequencing-Data Analysis for Cellulose- and Xylan-Degrading Enzymes from Pome Metagenome

Benbelgacem¹,

Isa²,

Abdelrahim³

et al. 2018

JSM

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Metagenomic DNA library from palm oil mill effluent (POME) was constructed and subjected to high-throughput screening to find genes encoding cellulose-and xylan-degrading enzymes. DNA of 30 positive fosmid clones were sequenced with next generation sequencing technology and the raw data (short insert-paired) was analyzed with bioinformatic tools. First, the quality of 64,821,599 reverse and forward sequences of 101 bp length raw data was tested using Fastqc and SOLEXA. Then, raw data filtering was carried out by trimming low quality values and short reads and the vector sequences were removed and again the output was checked and the trimming was repeated until a high quality read sets was obtained. The second step was the de novo assembly of sequences to reconstruct 2900 contigs following de Bruijn graph algorithm. Pre-assembled contigs were arranged in order, the distances between contigs were identified and oriented with SSPACE, where 2139 scaffolds have been reconstructed. 16,386 genes have been identified after gene prediction using Prodigal and putative ID assignment with Blastp vs NR protein. The acceptable strategy to handle metagenomic NGS-data in order to detect known and potentially unknown genes is presented and we showed the computational efficiency of de Bruijn graph algorithm of de novo assembly to 21 bioprospect genes encoding cellulose-degrading enzymes and 6 genes encoding xylan-degrading enzymes of 30.3% to 100% identity percentage.

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“…Several cell lines including primary chicken, duck embryo cells, and lines of LLCMK2, Vero, AP61 and C6/36 cells are continuously used (Tiwari et al, 2012;Minakshi et al, 2014). However, virus isolation techniques are time-consuming and require a tedious procedure that demands more than a week for completion.…”

Section: Antigen Detection Assaysmentioning

confidence: 99%