New approaches for fabrication of microfluidic capillary electrophoresis devices with on-chip conductivity detection

Guijt, Rosanne M.; Baltussen, Erik; Steen, G. van der; Schasfoort, Richardus B.M.; Schlautmann, Stefan; Billiet, H.A.H.; Frank, Johannes; Dedem, G.W.K. van; Berg, Albert van den

doi:10.1002/1522-2683(200101)22:2<235::aid-elps235>3.0.co;2-o

Cited by 117 publications

(95 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Interestingly, however, the results presented in this study compare favorably with microchip cation separations under similar conditions reported by other researchers [27,41,49]. The plate numbers obtained by Pumera et al [41] for Na 1 are also on the order of 45 000-55 000 for applied field strengths of 100 to 1000 V/cm.…”

Section: Cation Separationmentioning

confidence: 99%

A microchip electrophoresis system with integrated in-plane electrodes for contactless conductivity detection

2002

View full text Add to dashboard Cite

We present a new approach for contactless conductivity detection for microchip-based capillary electrophoresis (CE). The detector integrates easily with well-known microfabrication techniques for glass-based microfluidic devices. Platinum electrodes are structured in recesses in-plane with the microchannel network after glass etching, which allows precise positioning and batch fabrication of the electrodes. A thin glass wall of 10-15 microm separates the electrodes and the buffer electrolyte in the separation channel to achieve the electrical insulation necessary for contactless operation. The effective separation length is 34 mm, with a channel width of 50 microm and depth of 12 microm. Microchip CE devices with conductivity detection were characterized in terms of sensitivity and linearity of response, and were tested using samples containing up to three small cations. The limit of detection for K+ (18 microM) is good, though an order of magnitude higher than for comparable capillary-based systems and one recently reported example of contactless conductivity on chip. However, an integrated field-amplified stacking step could be employed prior to CE to preconcentrate the sample ions by a factor of four.

show abstract

Section: Cation Separationmentioning

confidence: 99%

A microchip electrophoresis system with integrated in-plane electrodes for contactless conductivity detection

2002

View full text Add to dashboard Cite

show abstract

“…Guijt et al [24] employed thin films and sacrificial etching to make silicon nitride/glass microchannels. Features etched into a silicon substrate had a thin film of silicon nitride deposited on top.…”

Section: Thin-film Sacrificial Layer Microfluidicsmentioning

confidence: 99%

Sacrificial layer microfluidic device fabrication methods

et al. 2006

View full text Add to dashboard Cite

Over the past fifteen years, research in the field of microfluidics has experienced rapid growth due to significant potential advantages such as low cost, short analysis times and elimination of sources of contamination. Whereas etched and thermally bonded glass substrates have seen widespread use and offer solid performance, device fabrication remains cumbersome. Recent advances in sacrificial layer microfabrication methods for microfluidics should overcome many disadvantages of conventional fabrication approaches. Phase-changing sacrificial layers have been implemented in making inexpensive and high-performance polymer microchips for electrophoretic analysis, protein focusing and sample preconcentration. In addition, novel channel fabrication methods based on standard thin-film processes, which are readily integratable with microfabrication techniques used for electrical components, are being applied increasingly for the creation of microfluidic devices. These new sacrificial layer fabrication approaches will be instrumental in making low-cost and highquality polymer microchips, and in interfacing electrical and fluidic systems on glass or semiconductor substrates.

show abstract

“…Van den Berg and co-workers [85][86][87] have manufactured glass-chips for zone electrophoresis and demonstrated the detection or inorganic ions and peptides with embedded electrodes. Soper and co-workers [88] have designed a device in PMMA and shown the separation and sensitive detection of amino acids, proteins and DNA fragments with a built-in conventional contacting conductivity detector.…”

Section: Figure 5 Schematic Drawing Of a C 4 Dmentioning

confidence: 99%

Conductimetric and potentiometric detection in conventional and microchip capillary electrophoresis

2002

View full text Add to dashboard Cite

Potentiometric detection is rarely used in separation methods but is promising for certain classes of analytes which can only with difficulty be quantified by more standard methods. Conductimetric detection of ions is very versatile and has recently received renewed interest spurned by the introduction of the capacitively coupled contactless configuration. Both are useful and complementary alternatives to the established optical detection methods, and to the more widely known electrochemical method of amperometry. The simplicity of the electrochemical methods makes them particularly attractive for microfabricated devices, but relatively little work has to date been carried out with regard to potentiometric and conductimetric detection.

show abstract

New approaches for fabrication of microfluidic capillary electrophoresis devices with on-chip conductivity detection

Cited by 117 publications

References 21 publications

A microchip electrophoresis system with integrated in-plane electrodes for contactless conductivity detection

A microchip electrophoresis system with integrated in-plane electrodes for contactless conductivity detection

Sacrificial layer microfluidic device fabrication methods

Conductimetric and potentiometric detection in conventional and microchip capillary electrophoresis

Contact Info

Product

Resources

About