New Attachment Formation Following Controlled Tissue Regeneration Using Biodegradable Membranes

Magnusson, Ingvar; Batich, Christopher D.; Collins, Brigitte

doi:10.1902/jop.1988.59.1.1

Cited by 170 publications

(84 citation statements)

References 19 publications

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“…6) at 2 weeks post-surgery was significantly greater than for the HA 0% group (Exp. 1) or HA 30% group (Exp. 2).…”

Section: Histometric Analysismentioning

confidence: 99%

“…Guided tissue regeneration (GTR) therapy was introduced in the 1980s to achieve a repopulation of the periodontal ligament fibroblasts, and was shown to promote periodontal regeneration. The membrane barrier used in GTR should be histocompatible, biocompatible and have the capacity for space maintenance 1) . Absorbable membrane does not require a second surgical procedure, and membrane exposure is rare.…”

Section: Introductionmentioning

confidence: 99%

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The Effects of Hydroxyapatite-Chitosan Membrane on Bone Regeneration in Rat Calvarial Defects

Shin

Choi

Kim

et al. 2009

J Korean Acad Periodontol

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Purpose: Recently, interest in chitosan has increased due to its excellent biological properties such as biocompatibility, antibacterial effect, and rapid healing capacity. On the other hand, hydroxyapatite is used as a bone substitute in the fields of orthopedics and dentistry. The hydroxyapatite-chitosan (HA-CS) complex containing hydroxyapatite nanoparticles was developed for synergy of both biomaterials. The objective of this study was to evaluate the effect of hydroxyapatite (HA)-chitosan (CS) membrane on bone regeneration in the rat calvarial defect. Methods: Eight-millimeter critical-sized calvarial defects were created in 70 male Sprague-Dawley rats. The animals were divided into 7 groups of 10 animals and received either 1) chitosan (CS) 100% membrane, 2) hydroxyapatite (HA) 30% / CS 70% membrane, 3) HA 30% / CS 70%, pressed membrane, 4) HA 40% / CS 60% membrane, 5) HA 50% / CS 50% membrane, 6) HA 50% / CS 50%, pressed membrane, or 7) a sham -surgery control. The amount of newly formed bone from the surface of the rat calvarial defects was measured using histomorphometry, following 2-or 8-week healing intervals. Results: Surgical implantation of the HA -CS membrane resulted in enhanced local bone formation at both 2 and 8 weeks compared to the control group. The HA -CS membrane would be significantly more effective than the chitosan membrane in early bone formation. Conclusions: Concerning the advantages of biomaterials, the HA-CS membrane would be an effective biomaterial for regeneration of periodontal bone. Further studies will be required to improve the mechanical properties to develop a more rigid scaffold for the HA-CS membrane. (J Korean Acad Periodontol 2009;39:213-222)

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“…6) at 2 weeks post-surgery was significantly greater than for the HA 0% group (Exp. 1) or HA 30% group (Exp. 2).…”

Section: Histometric Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Effects of Hydroxyapatite-Chitosan Membrane on Bone Regeneration in Rat Calvarial Defects

Shin

Choi

Kim

et al. 2009

J Korean Acad Periodontol

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“…Conventional periodontal treatment have been focused on periodontal tissue regeneration therapy to facilitate periodontal regeneration, such as bone graft [7], guided tissue regeneration (GTR) [8], and stem cell therapy [9], among which the GTR has become the most promising therapy and was widely used in clinical treatment for its convenience and effectiveness [10][11][12][13][14]. GTR treatment of periodontitis is a surgical procedure to implant a regeneration membrane that specifically aims to restore the periodontal tissues supporting the teeth [10].…”

Section: Introductionmentioning

confidence: 99%

“…In the history of developing the GTR membrane, nonresorbable materials were the first to be used in clinical treatment, such as the expanded-polytetrafluoroethylene (e-PTFE) [13]. However, materials such as e-PTFE are generally difficult to manipulate and required sutures around the necks of teeth to hold them in place.…”

Section: Introductionmentioning

confidence: 99%

Biodegradable electrospun PLLA/chitosan membrane as guided tissue regeneration membrane for treating periodontitis

et al. 2013

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This paper explores the application potential of a biodegradable PLLA/chitosan electrospun composite membrane for guided periodontal tissue regeneration which in addition serves as a fibroblast barrier. Electrospinning was applied to fabricate the PLLA membrane and aminolysis method was applied to graft chitosan on its surface. The morphology of the PLLA/chitosan membrane was observed by SEM. The surface chemical composition was analyzed by XPS. The appearance of N 1s peak in XPS demonstrated the successful grafting of chitosan on the PLLA electrospin membrane. After the modification, the water contact angle decreased from 136.9 ± 2.18°to 117.0 ± 2.10°, representing an improved hydrophilicity of the membrane. The bioactivity of the membrane was analyzed by XPS after soaking in SBF. The deposits had a Ca/P ratio of 1.6, indicating the hydroxyapatite formation on PLLA/chitosan membrane. The degradation rate was determined by measuring mass loss after immersion in PBS at different time periods. Compared to pure PLLA electrospun membrane which was almost nondegradable, the degradation rate of PLLA/chitosan composite membrane was up to 20 % in 6 weeks while maintaining its basic architecture to keep supporting the regenerated tissue. Live-dead cell staining of MC3T3 E1 cells cultured on the surface of the membrane showed a good biocompatibility of the PLLA/chitosan membrane. Furthermore, fibroblast cell line NIH 3T3 was cultured on surface of the membrane for the evaluation of cell penetration. The result demonstrated that the membrane worked as a fibroblast barrier to minimize the unfavorable effect of fibroblasts on periodontal tissue regeneration. Therefore, this electrospun PLLA/chitosan composite membrane has more potential for clinical application compared to old generation regeneration membrane with both suitable degradation rate and non-fibroblast penetration property.

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“…A variety of resorbable and non-resorbable barrier membranes have been utilized as regenerative materials based on the principles of GTR. To avoid a second surgical operation solely for removal of non-resorbable membranes, several resorbable membranes have been developed, of which collagen and polylactic acid membranes have shown promising results (5)(6)(7)(8)(9)(10).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of initial attachment of human gingival fibroblast cells to biodegradable membranes in vitro by light and scanning electron microscopy.

Özcan¹,

Tüter²,

Kurtiş³

et al. 1999

Journal of Oral Science

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Abstract:Guided tissue regeneration procedures using resorbable membranes have become accepted therapy for treating periodontal defects. Resorbable collagen and synthetic polylactide and polyglycolide copolymer membranes have been found to support regeneration and preclude the need for surgical removal. This study was undertaken to assess and compare the initial attachment of human gingival fibroblast cells to four collagen-based membranes (fascia lata, fascia temporalis, dura mater, and Type I bovine collagen) and a synthetic polylactic acid-based membrane (resolutR). Human gingival fibroblasts were grown from explants of normal tissue obtained during surgical reduction of retromolar tissues. Membrane specimens were placed in separate culture wells and incubated with fibroblasts for one hour. The number of adherent cells was evaluated by light microscopy using an ocular grid system and detailed examination was performed by scanning electron microscopy. The results of evaluation by light microscopy indicated that initial cell attachment was significantly less in the polylactic acid-based membrane group than in the collagen-based membrane groups (P<0.01).However, no significant differences were found among the collagen membrane groups in terms of fibroblast attachment (P>0.01).Scanning electron microscopy examination of fibroblasts cultured directly on barrier membranes indicated that the collagen-based membranes appeared to facilitate cell attachment, whereas the polylactic acid-based membrane exhibited a morphology that was not conducive to attachment of human gingival fibroblasts.Based on these limited in vitro results, it appears that collagen-based membranes offer greater potential than polylactic acid-based membranes for guided tissue regeneration at surgical sites. (J. Oral Sci. 41, 57-60, 1999)

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New Attachment Formation Following Controlled Tissue Regeneration Using Biodegradable Membranes

Cited by 170 publications

References 19 publications

The Effects of Hydroxyapatite-Chitosan Membrane on Bone Regeneration in Rat Calvarial Defects

The Effects of Hydroxyapatite-Chitosan Membrane on Bone Regeneration in Rat Calvarial Defects

Biodegradable electrospun PLLA/chitosan membrane as guided tissue regeneration membrane for treating periodontitis

Evaluation of initial attachment of human gingival fibroblast cells to biodegradable membranes in vitro by light and scanning electron microscopy.

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