New Bacteriophage of Multidrug Resistant Strain of Pseudomonas Aeruginosa

Abstract

Aim: Antimicrobial resistance in bacteria is one of the most discussed and important theme in healthcare field now. Bacteriophages are known as the most promising alternative to antibiotics and a method of fighting the multi-resistant pathogens. In this study, our main aim was to isolate lytic specific phages of multidrug resistant Pseudomonas aeruginosa, one of the most dangerous bacterial human pathogens. Methods: Disk-diffusion method, spot-test, agar overlay method, electron microscopy. Results: Antimicrobial resistance range of P. aeruginosa 458 strain was tested and it appeared to be resistant to all available antibiotics. Phages were isolated from waste waters in Kyiv. They appeared to have a typical morphology of Myoviridae family. Isolated viruses successfully lysed all the bacteria in vitro. Conclusions: Considering high effectiveness of isolated phage in vitro, we propose it as a candidate for phage therapy, though further studies on physical and genetic characteristics are required.