New basal media for half-anther culture of Anthurium andreanum Linden ex André cv. Tropical

Abstract

A successful protocol for high frequency callus induction and plant regeneration from Anthurium andreanum Linden ex André cv. Tropical half-anthers is described. Different variables using Winarto and Teixeira and Murashige and Skoog basal media supplemented with several plant growth regulators [2,4-dichlorophenoxy acetic acid (0.1-1.0 mg/l), a-naphthalene acetic acid (0.01-0.2 mg/l), thidiazuron (0.5-2.0 mg/l), 6-benzylaminopurine (0.5-1.0 mg/l), and kinetin (0.5-1.0 mg/l)] were tested for their ability to induce high frequency callusing in half-anthers, indirect regeneration and rooting of shoots. Basal medium, as well as the combination and concentration of hormones applied, had a significant effect on callus formation, shoot regeneration and adventitious root formation. Winarto and Teixeira-1, an original basal medium containing 0.01 mg/l a-naphthalene acetic acid, 0.5 mg/l thidiazuron and 1.0 mg/l 6-benzylaminopurine was suitable for callus formation while an improved basal medium i.e., New Winarto-Teixeira-3 supplemented with 0.25 mg/l 2,4-dichlorophenoxy acetic acid, 0.02 mg/l a-naphthalene acetic acid, 1.5 mg/l thidiazuron and 0.75 mg/l 6-benzylaminopurine enhanced callus formation. High shoot regeneration and multiplication was also possible on New Winarto-Teixeira-3. Shoots formed a strong adventitious root system on New Winarto-Teixeira-3 containing 0.2 mg/l a-naphthalene acetic acid and 1.0 mg/l kinetin. Plantlets that varied in size and performance were successfully acclimatized and adapted to ex vitro conditions. Cytological analysis of 180 acclimatized-plantlets ex vitro revealed that 34 were haploid (n = 14-18), 15 aneuploid (n = 20-26), 126 diploid (n = 28-34) and 5 triploid (n = 45-57). The potential use of this protocol for developing half-anther culture of other Anthurium species or cultivars is discussed.

Micropropagation of Anthurium spp.

“…In preliminary studies, induction and regeneration of callus followed by shoot and root regeneration are the main steps of tissue culture of whole plants. As an important commercial plant, to develop a rapid and more effective tissue culture protocol to shorten the time is the main objective of Anthurium tissue culture [7,10,22,23].…”

“…is Benlate], Cetrimite, gentamicin and streptomycin sulphate are effectively used for this aim [11,13,15,18,20,22]. Each plant species has its own medium composition or it should be improved for better results.…”

Perbanyakan Massa Anggrek Dendrobium Gradita 10 Secara In Vitro Melalui Embriogenesis Somatik

“…Mata tunas dan daun dipotong berbentuk kubus (0,5-1,00 cm) dan dikultur pada media Winarto-Teixeira (WT) (Winarto et al 2011) yang mengandung 2 mg/l TDZ, 0,5 mg/l BA, dan 0,02 mg/l NAA baik cair maupun padat. Tunas baru terbentuk ± 2 bulan setelah kultur dan selanjutnya dipanen daunnya sebagai sumber eksplan.…”

New Basal Media for Protocorm-Like Body and Callus Induction of Hybrid Cymbidium

“…All media were adjusted to pH 5.3 with 1 N NaOH or HCL prior to autoclaving at 100 KPa for 17 min. Cultures were kept on 40 ml medium in 100 ml Two new PLB-induction media, Teixeira Cymbidium (TC) medium, TC-1 and TC-2, inspired by but different to Winarto-Teixeira (WT) medium for Anthurium (Winarto et al, 2011), were tested against the industry standards, VW and half-strength MS (Tab. 1).…”

“…Namely, VW medium and similar media with weak concentrations of macro-and micro-nutrients (TC-1 or TC PLB in this study), were necessary for PLB induction and formation while higher levels of these nutrients were required for callus formation, namely MS, 1/2MS and TC-2 (i.e., TC CALLUS ). Winarto et al (2011) developed, though extensive media testing, a new medium, Winarto-Teixeira (WT) medium, for Anthurium. Several studies, including this study, have indicated that basal medium is key to the success of a tissue culture protocol.…”