New bioactive flavonoid glycosides isolated from the seeds of Lepidium apetalum Willd

Shi, Ping‐Ping; Chao, Li-Ping; Wang, Tingtxing; Liu, Erwei; Han, Lifeng; Qi, Zong; Li, Xiaoxia; Zhang, Yi; Wang, Tao

doi:10.1016/j.fitote.2015.04.007

Cited by 19 publications

(24 citation statements)

References 4 publications

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“…Several studies have reported that Lepidium apetalum Willd contains various types of secondary metabolites, such as oils, flavonoids, sterols and cardiac glycosides, but the basis of its efficacy are the flavonoids and flavonoid glycoside compounds [ 37 ]. A number of active ingredients were obtained from Lepidium apetalum Willd in our research group; among them, cis -DG and trans -DG significantly promoted the proliferation of NRK52e cells with a hypertonic model.…”

Section: Discussionmentioning

confidence: 99%

Two Sulfur Glycoside Compounds Isolated from Lepidium apetalum Willd Protect NRK52e Cells against Hypertonic-Induced Adhesion and Inflammation by Suppressing the MAPK Signaling Pathway and RAAS

Yuan

Zheng

et al. 2017

Molecules

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Lepidium apetalum Willd has been used to reduce edema and promote urination. Cis-desulfoglucotropaeolin (cis-DG) and trans-desulfoglucotropaeolin (trans-DG) were isolated from Lepidium apetalum Willd, and caused a significant increase in cell viability in a hypertonic model in NRK52e cells. In the hypertonic model, cis-DG and trans-DG significantly promoted the cell viability of NRK52e cells and inhibited the elevation of Na+ in the supernatant, inhibited the renin-angiotensin-aldosterone (RAAS) system, significantly reduced the levels of angiotensin II (Ang II) and aldosterone (ALD), and lowered aquaporin-2 (AQP2) and Na+–K+ ATP content in renal medulla. After treatment with cis-DG and trans-DG, expression of calcineurin (CAN) and Ca/calmodulin-dependent protein kinase II (CaMK II) was decreased in renal tissue and Ca2+ influx was inhibited, thereby reducing the secretion of transforming growth factor-β (TGFβ), reversing the increase in adhesion and inflammatory factor E-selectin and monocyte chemotactic protein 1 (MCP-1) induced by high NaCl, while reducing oxidative stress status and decreasing the expression of cyclooxygenase-2 (COX2). Furthermore, inhibition of protein kinase C (PKC) expression also contributed to these improvements. The cis-DG and trans-DG reduced the expression of p-p44/42 MAPK, p-JNK and p-p38, inhibited the phosphorylation of the MAPK signaling pathway in NRN52e cells induced by high salt, decreased the overexpression of p-p38 and p-HSP27, and inhibited the overactivation of the p38-MAPK signaling pathway, suggesting that the p38-MAPK pathway may play a vital role in the hypertonic-induced adhesion and inflammatory response. From the results of this study, it can be concluded that the mechanism of cis-DG and trans-DG may mainly be through inhibiting the p38-MAPK signaling pathway, inhibiting the excessive activation of the RAAS system, and thereby reducing adhesion and inflammatory factors.

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Section: Discussionmentioning

confidence: 99%

Two Sulfur Glycoside Compounds Isolated from Lepidium apetalum Willd Protect NRK52e Cells against Hypertonic-Induced Adhesion and Inflammation by Suppressing the MAPK Signaling Pathway and RAAS

Yuan

Zheng

et al. 2017

Molecules

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“…Lepidium apetalum Wild, a member of Cruciferae, is an annual or biennial weed with the height of 10-25 cm, flowering and fruiting from April to June [19]. This species is distributed throughout Asia and Europe [19][20][21]. Its natural habitats include roadsides, slopes, ravines, cultivated fields, and other human disturbed habitats [19].…”

Section: Introductionmentioning

confidence: 99%

“…Its natural habitats include roadsides, slopes, ravines, cultivated fields, and other human disturbed habitats [19]. Modern pharmacological studies have shown that seeds of L. apetalum have anti-oxidant, antibacterial, and cardiac activities [21,22]. Moreover, the seeds contained various secondary metabolites, such as oil, flavonoids, sterols and cardiac glycosides making it an important resource to develop and take advantage of [21,22].…”

Section: Introductionmentioning

confidence: 99%

Seed Biology of Lepidium apetalum (Brassicaceae), with Particular Reference to Dormancy and Mucilage Development

Zhang

Yin

et al. 2020

Plants

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Lepidium apetalum (Brassicaceae) is an annual or biennial weed widely distributed in Asia and Europe. The outer surface of L. apetalum seeds produces a large amount of mucilage. The primary aim of this study was to explore the dormancy characteristics and to determine how mucilage develops. The role of mucilage in water absorption/dehydration, the effects of after-ripening, gibberellin acid (GA3), cold stratification and seed coat scarification on germination, the role of mucilage in germination and seedling growth during drought, and the progress of mucilage production during seed development were investigated. The results indicate that the best temperature regime for germination was 10/20 °C. After-ripening, GA3 and seed coat scarification helped to break dormancy. Light promoted germination. Seedling growth of mucilaged seeds were significantly higher than those of demucilaged seeds at −0.606 and −1.027 MPa. Anatomical changes during seed development showed that mucilage was derived from the outer layer of the outer integument cells. Our findings suggest that seeds of L. apetalum exhibited non-deep physiological dormancy. The dormancy characteristics along with mucilage production give seeds of L. apetalum a competitive advantage over other species, and thus contribute to its potential as a weed. Effective control of this weed can be achieved by deep tillage.

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“…Compared with the seeds of L. apetalum , the previous investigations of herb-Sophia semen were more comprehensive [ 4 – 9 ]. Modern pharmacology has demonstrated that the seeds of L. apetalum have a cardiotonic [ 10 ] effect, and the chemical constituents are mainly flavonoids [ 11 – 15 ].…”

Section: Introductionmentioning

confidence: 99%

Lepidiumuridine A: A New Natural Uridine Derivative as a Phytoestrogen Isolated from the Seeds of Lepidium apetalum Willd.

Zeng

Zhang

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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There has been great interest in phytoestrogens, which are polyhydric compounds that are derived from plants and have a structure similar to that of the mammalian steroid hormone 17β-estradiol. The present study examined the estrogenic effects of a new natural uridine derivative, lepidiumuridine A (LA), that was isolated from the seeds of Lepidium apetalum. The structure was clarified and determined via analysis of extensive spectroscopic data interpretation. The activity of LA was investigated by measuring the levels of estradiol (E2), luteinizing hormone (LH), follicle stimulating hormone (FSH), and the uterus growth in mice. The proliferation experiment of MCF-7 breast cancer cells was also conducted. Western blot, in-cell western, and antagonist assays with methyl piperidino-pyrazole (MPP) were used for exploring the mechanism of the effects of LA. The results showed that LA elevated the uterine coefficient, the levels of E2, and FSH significantly. In addition, LA significantly elevated ERα expression in the uterus and MCF-7 cells. MPP inhibited the proliferation of LA-stimulated MCF-7 cell and ERα expression in MCF-7 cells. Taken together, LA had an estrogen-like effect, which was mainly mediated by the estrogen receptor ERα.

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New bioactive flavonoid glycosides isolated from the seeds of Lepidium apetalum Willd

Cited by 19 publications

References 4 publications

Two Sulfur Glycoside Compounds Isolated from Lepidium apetalum Willd Protect NRK52e Cells against Hypertonic-Induced Adhesion and Inflammation by Suppressing the MAPK Signaling Pathway and RAAS

Two Sulfur Glycoside Compounds Isolated from Lepidium apetalum Willd Protect NRK52e Cells against Hypertonic-Induced Adhesion and Inflammation by Suppressing the MAPK Signaling Pathway and RAAS

Seed Biology of Lepidium apetalum (Brassicaceae), with Particular Reference to Dormancy and Mucilage Development

Lepidiumuridine A: A New Natural Uridine Derivative as a Phytoestrogen Isolated from the Seeds of Lepidium apetalum Willd.

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