New Bioactive Taxoids from Cell Cultures of Taxus baccata

Abstract

Four new taxoids were isolated from cell cultures of Taxus baccata. Their structures were elucidated by spectroscopic analyses. Two were the aglycones corresponding to previously isolated 7-O-xylosides of taxol C [1] and 10-deacetyltaxol C [2]. The third [3] had an N-methylated side-chain, while the fourth, named taxcultine [4], contained an n-propyl group on the side-chain. All four compounds actively promoted tubulin assembly. Taxol C [1] showed potent and selective cytotoxicity in the NCI human cell line screen.

Screening for pharmaceutically important taxoids inTaxus baccata var.Aurea Corr. with CC/SPE/HPLC-PDA procedure

“…Column chromatography (CC) is often applied for the purposes of taxoids isolation from yew extracts and the silica gel is commonly used as the stationary phase (Vanhaelen-Fastre et al, 1992;Ma et al, 1994;Witherup et al, 1990). Reversed phases (C-18 bonded silica gel) are also applied (Rao et al, 1996).…”

Secondary metabolites production through biotechnological intervention: A Review

“…The first time, in vitro taxol production was carried out by the Christen et al (1989), thereafter, similar approached has been adopted in severeral research laboratories throughtout the world (Flores and Sgrignoli, 1991;Ma et al, 1994;Lee et al, 1995;Yukimune et al, 1996;Jha et al 1998;Nguyen et al, 2001;Wu et al, 2001;Linden et al, 2001;Parc et al, 2002;Abbasin et al, 2010).…”

Taxus Species (Yew): In Vitro Culture, and the Production of Taxol and Other Secondary Metabolites

“…At present, the main emphasis of tissue culture/biotechnology has been to establish cell cultures capable of producing taxol. The overall objective has been to establish fast-growing cultures that produce high levels Christen et al (1989Christen et al ( , 1991; Wickremesinhe (1992); Gibson et al (1993); Wickremesinhe and Arteca (1993a); Durzan and Ventimiglia (1994); Ellis et al (1994); Chee (1995); Ketchum et al (1995); Kim et al (1995Kim et al ( ) laziri et al (1991; Wickremesinhe (1992); Wickremesinhe and Arteca (1993a); Guo et al (1994); Ma et al (1994); Srinivasan et al (1995); Zhiri et al (1995a,b) Fett-Neto et al (1992) Fett-Neto et al (1992,1993,1995); Wickremesinhe (1992); ; Wickremesinhe and Arteca (1993a); Linden (1995,1996) Salandy (1993) Wickremesinhe and Arteca (1991, 1993b of taxol, which would eventually be suitable for scaleup and commercial production. A summary of callus/cell suspension cultures established from tissues of T. brevifalia, T. baccata, T. canadensis, T. cuspidata, and T. x media is presented in Table 2.…”