New Bone Formation Process Using Bio-Oss and Collagen Membrane for Rat Calvarial Bone Defect

Kasuya, Shin; Kato-Kogoe, Nahoko; Omori, Michi; Yamamoto, Kayoko; Taguchi, Shogo; Fujita, Hiroshi; Imagawa, Naoko; Sunano, Akihiro; Inoue, Kohei; Ito, Y.; Hirata, Azumi; Ueno, Takaaki; Moy, Peter K.

doi:10.1097/id.0000000000000738

Cited by 16 publications

(12 citation statements)

References 15 publications

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“…Such data led to the belief that bone substitute materials do not interfere with bone homeostasis. The present study shows that the long-term healing of murine alveolar bone in the presence of bovine bone displays typical histological findings of bone healing that are similar to those found in human (Carmagnola et al 2003) and animal models (Tovar et al 2014; Kasuya et al 2018). In addition, osteoclast staining shows insignificant elevation in cell numbers as opposed to the sham control, albeit at a level far from suggesting active bone processes (as seen at early healing phases in the same model) (Klein, Fleissig, et al 2019).…”

Section: Discussionsupporting

confidence: 82%

“…Healing of bone substitute materials in humans and animal models is commonly verified by histology. Indeed, current and classical evidence shows complete histological osseous healing (Carmagnola et al 2003; Tapety et al 2004; Kasuya et al 2018; Shi et al 2018) in the form of bone granule osseointegration at bone marrow spaces within osseous tissue (Carmagnola et al 2003). Such data led to the belief that bone substitute materials do not interfere with bone homeostasis.…”

Section: Discussionmentioning

confidence: 99%

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Bovine Bone Promotes Osseous Protection via Osteoclast Activation

et al. 2020

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The current study aimed at investigating the long-term biological mechanisms governing bone regeneration in osseous defects filled with bovine bone (BB). Tooth extraction sockets were filled with BB or left unfilled for natural healing in a C57BL/6 mouse alveolar regeneration bone model ( n = 12). Seven weeks later, the alveolar bone samples were analyzed histologically with hematoxylin/eosin and tartrate-resistant acid phosphatase staining. A separate group ( n = 10) was used for RNA sequencing. Osteoclast inhibition was induced by zoledronic acid (ZA) administration at 2 wk postextraction in a third group ( n = 28) for examination of osseous changes and cellular functions with micro–computed tomography and quantitative reverse transcription polymerase chain reaction, respectively. Histological and radiological osseous healing was observed in both BB-filled and normal-healing sockets. However, BB regenerated bone showed significant robust expression of genes associated with bone homeostasis and osteoclasts’ function. Osteoclasts’ inhibition in BB-filled sockets led to decreased bone resorption markers and reduced bone formation to a greater extent than that observed in osteoclasts’ inhibition with natural healing. BB displays long-term biologically active properties, despite a naive osseous histological appearance. These include activation of osteoclasts, which in turn promotes osseous remodeling and maturation of ossified bone.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Bovine Bone Promotes Osseous Protection via Osteoclast Activation

et al. 2020

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“…Geistlich's Bio-Oss is the most investigated bone substitute, characterized by desirable clinical results in comparison to other commercially available products. Despite positive clinical outcomes obtained with Bio-Oss, this material does not provide complete bone regeneration 7,8 . Furthermore, it has been shown that some particles remain within connective tissue for years 9,10 .…”

Section: Introductionmentioning

confidence: 95%

The influence of various coatings of hydroxyapatite bone carrier on the success of bone regeneration in rabbit calvarial defects: Histomorphometric and histological analysis

Milutinović-Smiljanić

Antonijević

Micic

et al. 2022

VOJNOSANIT PREGL

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The materials used nowadays for bone replacement do not fully meet the requirements for complete regeneration, which is why new ones are being tested. Background/Aim. Despite numerous attempts to improve bone tissue regeneration, no fulfilling material has been found yet. This study investigated the influence of poly-lactide-co-glycolide (PLGA) and poly-ethylene-imine (PEI) as coatings for hydroxyapatite (HAP) bone carrier onto bone tissue regenerative potential in rabbit's calvarial defect. Methods. Calvarial defects measuring 6 mm in diameter were made in 19 skeletally mature rabbits. Defects were filled with one of the following materials: PLGA coated HAP (HAP+PLGA), PEI coated HAP (HAP+PEI) and bovine HAP - Bio-Oss (positive control). Unfilled defects represented negative control. Histological analysis was performed in order to determine the inflammatory response of the host tissue. Formation of the new bone was evaluated by means of histomorphometric analysis. All analysis have been conducted in samples obtained 3, 6 and 9 weeks after implantation. Results. Three weeks post-implantation, a trend toward increased healing in HAP+PLGA group compared to other investigated materials was noticed, with no statistically significant difference between study groups (p>0.05). However, after six and 9 weeks, significant healing was observed in favour of the HAP coated with PLGA compared to other groups (p<0.05). Within this group, greater bone healing was observed comparing to HAP+PEI and Bio-Oss. Conclusion. PLGA has demonstrated greater coating potential comparing to PEI with respect to osteogenesis improvement in bone reconstructive surgery does.

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“…The dura mater injury can lead to impairment of the healing process, due the function of being the main osteogenic cells and osteoinductive substances source 52 . Considering the defects, these are performed with a surgical trephine bur, the length of which can vary from 5 to 8 mm in diameter, extending into the two bone cortical -central defect [53][54][55][56] . Still regarding size, there is no consensus in the literature as to the ideal size, however there is a tendency for the creation of central defects with a diameter of 5-8 mm [47][48][49]57,58 .…”

Section: Bone Repair and Photobiomodulationmentioning

confidence: 99%

Effects of Photobiomodulation on Experimental Bone Repair in Animal models: a Systematic Review

Neto¹,

Lilge²,

Damante³

et al. 2021

JMS

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Introduction: photobiomodulation was showed positive effects on bone healing, however, several studies search safe protocols and dosimetry, beyond good experimental models. Thus, the present study aimed to conduct a systematic review of photobiomodulation (PBM) effects on bone healing increase, focusing on animal experimental models. Revision: the studies search on the PBM use in bone repair was carried out in the PubMed / MEDLINE and Lilacs databases, excluding studies that included systemic conditions and biomaterials or medications use. Sixteen studies were selected, within the inclusion and exclusion criteria stipulated. Animal models included rats (n = 14) and rabbits (n = 2). The laser parameters applied ranging between 650 nm and 830 nm and the power density ranging from 0.03 W to 6 W. Animal models were mainly concentrated in rodents, and the most utilized defect type was the tibial bone defect with 2.5 mm of diameter. Conclusion: through this review we concluded that the location and size of the defects, as well as the laser irradiation parameters are diverse and have shown the lack of consensus on the topic, however the results for using PBM therapy are encouraging.

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New Bone Formation Process Using Bio-Oss and Collagen Membrane for Rat Calvarial Bone Defect

Abstract: Our results suggested that the Bio-Oss acts as a scaffold for bone repair, and the use of a collagen membrane may anchor the Bio-Oss closely to the cranial bone and assist the bone repair response.

Cited by 16 publications

References 15 publications

Bovine Bone Promotes Osseous Protection via Osteoclast Activation

Bovine Bone Promotes Osseous Protection via Osteoclast Activation

The influence of various coatings of hydroxyapatite bone carrier on the success of bone regeneration in rabbit calvarial defects: Histomorphometric and histological analysis

Effects of Photobiomodulation on Experimental Bone Repair in Animal models: a Systematic Review

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