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Atmospheric pressure plasma has the potential of being applied in pre-harvest processes as a sustainable technology for seed and plant treatment. In this study, a medium-scale volume dielectric barrier discharge reactor with a power density of 53.5 mW/cm3 was developed and applied for continuous treatment of larger amount of seeds. Within the argon plasma, reactive oxygen and nitrogen species were observed by optical emission spectroscopy. Short-term plasma treatment in the range of seconds of wheat and barley seeds revealed improved germination performance accompanied by altered seed surface properties. Depending on the exposure time, plasma displayed a positive impact on maximum germination and germination speed in both species. In line with this, the t50 values were significantly decreased for plasma treated seeds compared to untreated seeds for wheat and barley. Cumulative germination was enhanced from 20% up to 53% after 27 h of germination for wheat seeds treated from 10 s to 60 s with plasma. Barley seeds respond to the short plasma treatment times with increased germination of 10%–23% after 24 h observation time. Moreover, the seed surface of both plant species became more hydrophilic after plasma treatment supported by a decrease in the water contact angle and an increase in hydrophilic functional groups being detected by x-ray photoelectron spectroscopy. Water uptake by seeds was moderately increased after 2 h of imbibition for both plant species. The positive effects of plasma treatment on germination performance were still detectable after storage of seeds for one and two months.
Atmospheric pressure plasma has the potential of being applied in pre-harvest processes as a sustainable technology for seed and plant treatment. In this study, a medium-scale volume dielectric barrier discharge reactor with a power density of 53.5 mW/cm3 was developed and applied for continuous treatment of larger amount of seeds. Within the argon plasma, reactive oxygen and nitrogen species were observed by optical emission spectroscopy. Short-term plasma treatment in the range of seconds of wheat and barley seeds revealed improved germination performance accompanied by altered seed surface properties. Depending on the exposure time, plasma displayed a positive impact on maximum germination and germination speed in both species. In line with this, the t50 values were significantly decreased for plasma treated seeds compared to untreated seeds for wheat and barley. Cumulative germination was enhanced from 20% up to 53% after 27 h of germination for wheat seeds treated from 10 s to 60 s with plasma. Barley seeds respond to the short plasma treatment times with increased germination of 10%–23% after 24 h observation time. Moreover, the seed surface of both plant species became more hydrophilic after plasma treatment supported by a decrease in the water contact angle and an increase in hydrophilic functional groups being detected by x-ray photoelectron spectroscopy. Water uptake by seeds was moderately increased after 2 h of imbibition for both plant species. The positive effects of plasma treatment on germination performance were still detectable after storage of seeds for one and two months.
The increasing demand for qualitative and varietal foods by the consumer society is a big concern for energy production, and utilization of that energy in a judicious manner for sustainable management of resources is a big challenge in the eminent future. Existing resources (land, water, fertilizer, etc.) and their socioeconomic aspects warrant the farming community to adopt alternative strategies aimed at enhancing the use efficiency of inputs and improve the environmental quality. The adaptability of microbes to thrive in different environments has prompted scientists to introduce microbial intervention in the agricultural processes. Bio-priming has the potential to fulfill many objectives of the modern production system with the use of beneficial microorganisms in an eco-friendly manner. Interestingly, it also plays a crucial role in enhancing the nutrient use efficiency of crops. There is rising evidence of a paradigm shift from the use of a single microbe to a consortium approach for efficient rhizosphere engineering in the context of sustainable agriculture. Our understanding of different signaling cascades, rhizosphere chemistry, and other mechanisms of plant–microbial interactions will frame suitable strategies to harness the best ecosystem services including improved resource use efficiency.
Poor early growth and uneven crop establishment are reported as the major bottlenecks in wide-scale adoption and optimal yield realization of dry direct-seeded rice (DSR). Seed priming can potentially help overcome these problems in DSR. Therefore, laboratory and field studies were conducted at Punjab Agricultural University, Ludhiana, India, during kharif/wet-season 2018 and 2019 to evaluate the effect of different priming techniques on germination, establishment, growth, and grain yield of rice under DSR conditions. The following priming treatments were evaluated: dry non-primed seed (control), hydropriming with distilled water, halopriming with 2.0% potassium nitrate, hormopriming with 50 ppm gibberellic acid (GA3), and osmopriming with polyethylene glycol (PEG)(−0.6 MPa), each with 12 and 24 h priming duration. In 2019, priming treatments were tested under two DSR establishment methods—conventional DSR (sowing in dry soil followed by irrigation) and soil mulch DSR (locally known as vattar DSR) (sowing in moist soil after pre-sowing irrigation), whereas in 2018, priming treatments were evaluated under conventional DSR only. In both years, halopriming and hormopriming resulted in a 7–11% increase in rice yields compared to non-primed dry seed (control). Osmopriming resulted in a 4% yield increase compared to control in 2018 but not in 2019. The higher yields in halopriming and hormopriming were attributed to higher and rapid germination/crop emergence, better root growth, and improvement in yield attributes. Priming effect on crop emergence, growth, and yield did not differ by DSR establishment methods and duration of priming. Conventional DSR and soil mulch DSR did not differ in grain yield, whereas they differed in crop emergence, growth, and yield attributes. These results suggest that halopriming with 2.0% potassium nitrate and hormopriming with 50 ppm GA3 has good potential to improve crop establishment and yield of rice in both conventional and soil mulch DSR systems.
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