New Chromogenic Agar Medium for the Identification ofCandidaspp

Abstract

. On all three agars, colonies of C. albicans were not distinguished from colonies of C. dubliniensis. However, for the group containing C. albicans plus C. dubliniensis, both the sensitivity and the specificity of detection when CDA was used were 100%, compared with values of 97.6 and 100%, respectively, with CHROMagar Candida and 100 and 96.8%, respectively, with Candida ID agar. In addition, for the group containing C. tropicalis plus C. kefyr, the sensitivity and specificity of detection when CDA was used were also 100%, compared with 72.7 and 98.1%, respectively, with CHROMagar Candida. Candida ID agar did not differentiate C. tropicalis and C. kefyr strains but did differentiate members of a broader group (C. tropicalis, C. kefyr, Candida lusitaniae plus Candida guilliermondii); the sensitivity and specificity of detection for members of this group were 94.7 and 93.8%, respectively. In addition to the increased sensitivity and/or specificity of Candida detection when CDA was used, differentiation of colony types on CDA (red spotted, pink, or no color) was unambiguous and did not require precise assessment of colony color.

Evaluation of the New Chromogenic Medium Candida ID 2 for Isolation and Identification of Candida albicans and Other Medically Important Candida Species

“…This color divergence reinforced the different shade of green described by Schoofs et al (26) for C. dubliniensis. We have not been able to confirm this observation in a consistent way when using the CAC medium provided by CHROMagar (France), with results that are in agreement with recent publications (6,23). In the present study, we have evaluated many stock culture strains, and it has been reported that the dark green color of the colonies may be lost upon repeated subculture or storage (11,25,26).…”

Use of chromogenic medium in the isolation of yeasts from clinical specimens

“…We also described alternative colours of some yeasts on CaC that are not consistent with the traditionally identifiable colours. This may represent recovery of yeasts of different species on some plates or colour variability among strains of these species, as has been reported by others (Cooke et al, 2002). This reveals a limitation of our study in that we did not identify to the species level all yeasts detected on CaC in parallel with RM.…”

Utilização de um meio cromogênico e da técnica de semi-nested PCR para identificação de espécies de Cândida

“…Vários meios seletivos diferenciais cromogênicos para identificação presuntiva das amostras de Candida foram desenvolvidos (ODDS; BERNAERTS, 1994;COOKE et al, 2002;HORVATH et al, 2003;KHAN et al, 2004). Esses meios contêm substratos que reagem com enzimas secretadas pelos fungos produzindo colônias com várias pigmentações.…”