New classes of selective separations exploiting magnetic adsorbents

Franzreb, Matthias

doi:10.1016/j.cocis.2020.03.012

Cited by 18 publications

(11 citation statements)

References 49 publications

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“…Production costs of only a few US$ per 100 g nanoparticles are realistic at the lab scale. Therefore, from a processing point of view, magnetic materials represent a viable alternative, with regard to material expenses, processing time and volume, when designing processes with higher productivity. ,− The successful immobilization of ligands for antibodies as well as the direct antibody binding on magnetic particles has been demonstrated in various application fields, from biosensing to targeted drug delivery and others. ,− …”

Section: Introductionmentioning

confidence: 99%

Seeking Innovative Affinity Approaches: A Performance Comparison between Magnetic Nanoparticle Agglomerates and Chromatography Resins for Antibody Recovery

Padwal

Finger

Fraga-García

et al. 2020

ACS Appl. Mater. Interfaces

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Monoclonal antibodies are key molecules in medicine and pharmaceuticals. A potentially crucial drawback for faster advances in research here is their high price due to the extremely expensive antibody purification process, particularly the affinity capture step. Affinity chromatography materials have to demonstrate the high binding capacity and recovery efficiency as well as superior chemical and mechanical stability. Low-cost materials and robust, faster processes would reduce costs and enhance industrial immunoglobulin purification. Therefore, exploring the use of alternative materials is necessary. In this context, we conduct the first comparison of the performance of magnetic nanoparticles with commercially available chromatography resins and magnetic microparticles with regard to immobilizing Protein G ligands and recovering immunoglobulin G (IgG). Simultaneously, we demonstrate the suitability of bare as well as silica-coated and epoxy-functionalized magnetite nanoparticles for this purpose. All materials applied have a similar specific surface area but differ in the nature of their matrix and surface accessibility. The nanoparticles are present as micrometer agglomerates in solution. The highest Protein G density can be observed on the nanoparticles. IgG adsorbs as a multilayer on all materials investigated. However, the recovery of IgG after washing indicates a remaining monolayer, which points to the specificity of the IgG binding to the immobilized Protein G. One important finding is the impact of the ligand-binding stoichiometry (Protein G surface coverage) on IgG recovery, reusability, and the ability to withstand long-term sanitization. Differences in the materials’ performances are attributed to mass transfer limitations and steric hindrance. These results demonstrate that nanoparticles represent a promising material for the economical and efficient immobilization of proteins and the affinity purification of antibodies, promoting innovation in downstream processing.

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Section: Introductionmentioning

confidence: 99%

Seeking Innovative Affinity Approaches: A Performance Comparison between Magnetic Nanoparticle Agglomerates and Chromatography Resins for Antibody Recovery

Padwal

Finger

Fraga-García

et al. 2020

ACS Appl. Mater. Interfaces

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“…(2019) . Magnetic seeded filtration (MSF), also referred to as magnetic flocculation ( Tsouris et al., 1995 ) and magnetic adsorption ( Franzreb, 2020 ) is a solid-liquid separation process, which has been used in various applications ( Franzreb, 2020 ). The approach is based on the selective hetero-agglomeration between magnetic seed particles and the (nonmagnetic) target particles.…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies show that MSF is capable of achieving high separation efficiencies for microplastics Rhein et al. (2019b) under laboratory conditions and identified benefits included the high material specific selectivity and the applicability in dilute suspensions ( Franzreb, 2020 ; Rhein et al., 2019b ). However, as MSF has not yet been widely applied in large-scale applications, no general estimations on cost effectiveness exists, yet.…”

Section: Introductionmentioning

confidence: 99%

Separation of Microplastic Particles from Sewage Sludge Extracts Using Magnetic Seeded Filtration

2022

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“…A special discipline is the use of so-called magnetic nanoparticles (MNPs), with a rapidly increasing number of applications shown in multiple scientific topics. On a technical level, MNPs are progressingly used for sensor technology [1], separation and extraction techniques [2,3], magnetic bearings [4], catalysis [5,6] and wastewater treatment [7,8]. Especially in medicine, the use of MNPs for cancer therapy shows very promising application areas.…”

Section: Introductionmentioning

confidence: 99%

Magnetic/flow controlled continuous size fractionation of magnetic nanoparticles using simulated moving bed chromatography

Kuger

Arlt

Franzreb

2022

Talanta

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New classes of selective separations exploiting magnetic adsorbents

Cited by 18 publications

References 49 publications

Seeking Innovative Affinity Approaches: A Performance Comparison between Magnetic Nanoparticle Agglomerates and Chromatography Resins for Antibody Recovery

Seeking Innovative Affinity Approaches: A Performance Comparison between Magnetic Nanoparticle Agglomerates and Chromatography Resins for Antibody Recovery

Separation of Microplastic Particles from Sewage Sludge Extracts Using Magnetic Seeded Filtration

Magnetic/flow controlled continuous size fractionation of magnetic nanoparticles using simulated moving bed chromatography

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