New closed system using a sterile connection device and preconnected PRP pack for extended storage of apheresis platelet products.

Shimizu, Tetsuo; Kouketsu, Keiko; Morishima, Yasuo; Hasegawa, I; Kamiya, Tadashi

doi:10.1620/tjem.155.303

Cited by 4 publications

(2 citation statements)

References 4 publications

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“…Four hundred milliliter of platelet-rich plasma (PRP) was collect ed from healthy donors into a 0.6-liter polyolefin bag (Nissho Corp.. Osaka, Japan) by apheresis using a blood cell separator (Haemonetics, AA) and a standard procedure with ACD solution as an anti coagulant [14], A new closed system using SCD (Haemonetics/Dupont) was used for collection of PRP [15].…”

Section: Platelet Collectionmentioning

confidence: 99%

Plasma-Depleted Platelet Concentrates Prepared with a New Washing Solution

Shimizu¹,

Shibata²,

Kora³

1993

Vox Sanguinis

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In certain clinical situations, complete removal of the plasma proteins from the platelet concentrates (PCs) is necessary by washing prior to transfusion. A simple electrolyte solution with a pH of 6.5 was developed for washing PCs. The platelet-rich plasma collected with acid-citrate-dextrose solution by apheresis in a 0.6-liter polyolefin bag was centrifuged. After removal of the supernatant plasma from pelleted platelet buttons, 200 ml of a washing solution consisting of 90 mM NaCl, 5 mM KCl, 3 mM MgCl(2), 17 mM NaH(2)PO(4), 8 mM Na(2)HPO(4), 23 mM Na acetate, 17 mM Na(3) citrate, 23.5 mM glucose, 2 mM adenine, 0.1% dextran, and 28.8 mM maltose (pH 6.5) was added to the pelleted platelet button. Steam sterilization of the solution was carried out under nitrogen to avoid caramelization of glucose. After resuspension of the pelleted platelet button with a washing solution and a second centrifugation. Seto additive solution (Seto sol, pH 7.4) was introduced into the bag to resuspend the platelet buttons for storage for 3 days at 22°C. All of these procedures were completed within 3 h using a sterile docking device. In washed PCs, 99.1% of the plasma was removed and platelet recovery was 96%. The washed PCs were compared for 3 days with plasma-poor PCs consisting of 11% plasma and 89% Seto solution. There were no significant differences in percent hypotonic shock response, aggregation, energy metabolism, and morphology of platelets between the two groups during 3 days, except for significant swelling of 3-day- old platelets in washed PCs. We conclude that washing PCs with a newly developed medium and resuspension by Seto sol (pH 7.4) had minimum detri- menal effects on further storage for 3 days. It is indicated that the procedure described here is relatively simple and can be finished within 3 h after the collection of platelets, and since plasma removal was sufficiently high, a new plasma-depleted PC product may be a valuable therapeutic tool for hemostasis therapy with reduced side effects from plasma proteins.

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Section: Platelet Collectionmentioning

confidence: 99%

Plasma-Depleted Platelet Concentrates Prepared with a New Washing Solution

Shimizu¹,

Shibata²,

Kora³

1993

Vox Sanguinis

Self Cite

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“…We previously discussed a new closed system using a sterile connection device (SCD 312®, Du Pont) and pre connected PRP pack for extended storage of 5PC for 5 days [19], PRP packs included the apheresis bowl of the PCS system with a preconnected 16 gauge needle and double container system. The anticoagulant line of the PRP pack and the tube from the container containing ACD-A solution were welded by a sterile connection device.…”

Section: -mentioning

confidence: 99%

A Novel Second-Generation Polyolefin Container for Storage of Single-Donor Apheresis Platelets

Shimizu¹,

Kouketsu²,

Kamiya³

et al. 1989

Vox Sanguinis

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A non-blow-molded LE-2 polyolefin (PO) container was developed to store single-donor apheresis platelet concentrates (PCs) processed by the Haemonetics Plasma Collecting System (PCS) at 22°C for over 1 day. We molded PO containers with heat-sealing of light-weight polymer alloy films with a thickness of 0.25 mm. The film was made of polymer blends consisting of polypropylene, styrene ethylene butylene styrene-block copolymer, and ehtylene ethylacrylate copolymer. The PO container with enough strength and flexibility in routine practice has 2 and 1.6 times higher oxygen and carbon dioxide gas transfer properties than standard polyvinyl chloride (PVC) plastic containers. PCs (1-1.9x10^11 platelets) processed from 450ml platelet-rich plasma were stored in 0.6-liter PO containers at 22°C with flatbed agitation for up to 6 days. The pH of PCs was well maintained at the mean values of 7.0 in PO containers after 6 days in the well-oxygenated condition. The energy metabolism of stored platelets was determined. Oxygen consumption rates of platelets stored in PO containers averaged 1.5 nmol/min/10^9 platelets. The rates of glucose consumption and lactate production were 0.4 and 0.8 nmol/min/10^11 platelets, respectively. The rates of adenosine triphosphate (ATP) generation of platelets, 9.7 nmol/min/109 platelets, in PO containers did not differ from those in the PVC containers. Aggregation responses to adenosine diphosphate and hypotonic shock response of platelets were better maintained in PO containers. The morphological changes into sphere forms with projections and the appearance of unclassified forms were more frequently observed in PO than in PVC containers. Single-donor apheresis platelet products collected by the PCS system may be stored in a new 0.6-liter PO container for over 5 days under acceptable conditions.

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A Novel Second‐Generation Polyolefin Container for Storage of Single‐Donor Apheresis Platelets

et al. 1989

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A non-blow-molded LE-2 polyolefin (PO) container was developed to store single-donor apheresis platelet concentrates (PCs) processed by the Haemonetics Plasma Collecting System (PCS) at 22 degrees C for over 1 day. We molded PO containers with heat-sealing of light-weight polymer alloy films with a thickness of 0.25 mm. The film was made of polymer blends consisting of polypropylene, styrene ethylene butylene styrene-block copolymer, and ethylene ethylacrylate copolymer. The PO container with enough strength and flexibility in routine practice has 2 and 1.6 times higher oxygen and carbon dioxide gas transfer properties than standard polyvinyl chloride (PVC) plastic containers. PCs (1-1.9 x 10(11) platelets) processed from 450 ml platelet-rich plasma were stored in 0.6-liter PO containers at 22 degrees C with flatbed agitation for up to 6 days. The pH of PCs was well maintained at the mean values of 7.0 in PO containers after 6 days in the well-oxygenated condition. The energy metabolism of stored platelets was determined. Oxygen consumption rates of platelets stored in PO containers averaged 1.5 nmol/min/10(9) platelets. The rates of glucose consumption and lactate production were 0.4 and 0.8 nmol/min/10(11) platelets, respectively. The rates of adenosine triphosphate (ATP) generation of platelets, 9.7 nmol/min/10(9) platelets, in PO containers did not differ from those in the PVC containers. Aggregation responses to adenosine diphosphate and hypotonic shock response of platelets were better maintained in PO containers. The morphological changes into sphere forms with projections and the appearance of unclassified forms were more frequently observed in PO than in PVC containers.(ABSTRACT TRUNCATED AT 250 WORDS)

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New closed system using a sterile connection device and preconnected PRP pack for extended storage of apheresis platelet products.

Cited by 4 publications

References 4 publications

Plasma-Depleted Platelet Concentrates Prepared with a New Washing Solution

Plasma-Depleted Platelet Concentrates Prepared with a New Washing Solution

A Novel Second-Generation Polyolefin Container for Storage of Single-Donor Apheresis Platelets

A Novel Second‐Generation Polyolefin Container for Storage of Single‐Donor Apheresis Platelets

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