New colorimetric method to determine catalase mimic activity

Rasheed, Rashed Taleb; Mansoor, Hadeel Salah; Mansoor, Aseel S.

doi:10.1088/2053-1591/ab706b

Cited by 18 publications

(3 citation statements)

References 29 publications

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“…The authors developed a new spectrophotometric method to measure the catalase mimetic activity of V 2 O 5 solution [25,29,38] that included the use of acidic permanganate solution. In order to calculate the reaction rate, the catalase mimetic activity of V 2 O 5 was determined for samples asprepared at 90 °C and after annealing at different temperatures 250-750 °C for 120 min.…”

Section: Advanced Spectrophotometric Methods Developmentmentioning

confidence: 99%

Synthesis, characterization of V2O5 nanoparticles and determination of catalase mimetic activity by new colorimetric method

Rasheed

Mansoor

Abdullah

et al. 2020

J Therm Anal Calorim

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This paper deals with an advanced colorimetric method used to determine the catalase mimetic activity of V2O5 nanoparticles by measuring the decrease in potassium permanganate concentration in a mixture containing V2O5 and hydrogen peroxide. The experiments were carried out in batch reactor at room temperature for 3 min at wavelength number of 525 nm. Vanadium pentoxide was synthesized by hydrothermal method (reflux) from ammonium metavanadate (NH4VO3) as a precursor and cetyltrimethylammonium bromide as a surfactant. The annealing of the product was carried out for 2 h, at temperatures of 250, 500 and 750 °C. In order to determine the structure and the chemical nature of the nanoparticles prepared, the characterization was carried out by X-ray diffraction and scanning electron microscopic techniques. Atomic force microscopic and thermal gravimetric investigations have shown the decomposition steps of V2O5 at different temperatures. UV–visible spectroscopic technique and Fourier transform spectrometry were used to further characterize the nanoparticles. Advanced colorimetric method was used to study the catalase mimetic activity of the newly synthesized vanadium pentoxide (V2O5) nanoparticles using hydrogen peroxide (H2O2) as substrate. V2O5 nanoparticles resulted in an increase in the catalase mimetic activity with increasing the annealing temperature of the V2O5 nanoparticles. The maximum activity was found at 500 °C, which subsequently decreased with further increase in the annealing temperature.

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Section: Advanced Spectrophotometric Methods Developmentmentioning

confidence: 99%

Synthesis, characterization of V2O5 nanoparticles and determination of catalase mimetic activity by new colorimetric method

Rasheed

Mansoor

Abdullah

et al. 2020

J Therm Anal Calorim

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“…Catalase (CAT) mimetic activity was measured using absorbance by the Uv/Visible spectrometer technique at a wavelength of 240 nm [18] as follows: three (50 mL) containers labeled (T, C, and B) for each nanoparticle (Fe2O3 and Fe3O4). Both the T and B containers received (0.001gm) and (0.002 gm) of (Fe2O3 and Fe3O4), respectively.…”

Section: Measuring Enzymes 31 Measuring Catalase (Cat) Mimetic Activitymentioning

confidence: 99%

Preparation, Characterization and Mimetic Activities of Fe2O3 and Fe3O4 Nanoparticles as Catalase and Peroxidase

Majeed¹,

Rasheed²

2022

JASN

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Different iron oxide nanoparticles (Fe3O4 and Fe2O3) were prepared by the sol-gel method (titration). The prepared nanoparticles were heated at 90 and 400°C. The morphology surface and structures were characterized by Fourier Transform Infra-Red (FT-IR) and Ultraviolet/Visible (Uv/Visible) measurements, X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), and Atomic Force Microscope (AFM). The enzyme mimetic activities of these nanoparticles (Fe3O4 and Fe2O3) such as two enzymes (Catalase (CAT), and Peroxidase (Pxase)) were measured. The results showed the iron oxides (Fe2O3) heated at 90°C, have the maximum activity (189.99 K.min -1 ) as catalase (CAT). While the iron oxides (Fe3O4) heated at 90°C, have the maximum activity (3.044 U.min -1 ) as peroxidase (Pxase), and there is a decrease in the activity for both nanoparticles when annealed at 400°C. Despite the average grain size decrease in both samples, however, the mimetic activity decrease that is mean the average grain size is not affected in both mimetic activities as catalase and peroxidase.

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“…The biological property is shown by CuO nanoparticles are wound dressings and biocide properties [14], antibacterial [15]. Semiconductor materials were mainly interesting due to their great practical importance in optoelectronic devices and electronic such as magnetic storage devices, catalytic activity, catalase mimic, electro chemical cell [16][17][18][19], and at crystalline structure of the prepared CuO was characterized by diffraction (XRD), Atomic force microscopy (AFM) and antimicrobial application [20].…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of CuO Nanoparticles Prepared by Chemical Method

Rasheed

Jassim

Easa

et al. 2021

MSF

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Copper oxide (CuO) nanoparticles were prepared by the sol-gel method, by the reaction of copper chloride and ammonium hydroxide as procurers. Nanopowders are annealing at different temperatures (100 °C, 200 °C, and 600 °C) for 120 min and confirmed the monoclinic phase by X-ray diffraction analysis of the metal oxide with lattice parameters a = 4.694 Å, b = 3.456 Å and c = 5.165Å for annealing temperature 400°C. Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), and UV-Visible Spectroscopy spectrum was used to determine the structure, average crystallize, and morphology. We found increasing in size and energy gap (Eg) from (62.95 nm to 106.84 nm) and from (1.72 eV to 1.49 eV), when annealing temperature increasing from 200 °C to 600 °C, respectively.

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New colorimetric method to determine catalase mimic activity

Cited by 18 publications

References 29 publications

Synthesis, characterization of V2O5 nanoparticles and determination of catalase mimetic activity by new colorimetric method

Synthesis, characterization of V2O5 nanoparticles and determination of catalase mimetic activity by new colorimetric method

Preparation, Characterization and Mimetic Activities of Fe2O3 and Fe3O4 Nanoparticles as Catalase and Peroxidase

Preparation and Characterization of CuO Nanoparticles Prepared by Chemical Method

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