New Contributions to Experimental Asthma

Noelpp-Eschenhagen, I.; Noelpp, B.

doi:10.1159/000385894

Cited by 7 publications

(5 citation statements)

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“…Recently it has been shown that inhalation of ovalbumin aerosol significantly promoted the production of specific IgE in mice [31]. The massive participation of eosinophils, observed by us and others in allergic guinea pigs after challenge with the aerosolized specific antigen, speaks also for an IgE-mediated reaction [14,16,49,60], Anyway, the histological changes we found are identical with those occurring in human type I aller gic asthma. Their model character seems to us to be well established, despite the uncertainty concerning the antibody class mediating them and the nonpersistent, time-limited course of the allergic sensitization in guinea pigs.…”

Section: IIsupporting

confidence: 72%

“…1,60,73,77], It was concluded that anaphylactic sensiti zation and the elicitation of anaphylactic pneumonia or shock by inhalation of the homologous antigen was possible. Kallós and Pagel [47] employed a modi fication of the method originally described by Alexan 1 Dedicated to the memory of our late friend and co-worker.…”

Section: Imentioning

confidence: 99%

“…Important progress has been made by NoelppEschenhagen and Noelpp [60], who introduced record ing techniques, to allow objective observation of the course of the asthmatic attacks in guinea pigs and their quantitative evaluation. Different ventilatory and respiratory parameters, e.g.…”

Section: IVmentioning

confidence: 99%

“…This is regarded as characteristic for human asthma [59] and adds to the model character of the experimental dis ease. It has also been observed that guinea pigs be came more sensitive to a histamine aerosol after re peated allergic asthma attacks [69,70], In other words, these animals developed 'bronchial hyperreactivity', another important characteristic of human asthma [59], In the course of their investigations, Noelpp-Eschenhagen and Noelpp [60] made an important dis covery. They noted that a few guinea pigs repeatedly exposed to inhalation of asthmogenic aerosols devel oped 'learned asthma', i.e.…”

Section: IVmentioning

confidence: 99%

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Experimental Asthma in Guinea Pigs Revisited

Kallós¹,

Kallós²

1984

Int Arch Allergy Immunol

101

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Experimental asthma in guinea pigs, as defined in 1937 by Kallós and Pagel, and the pertinent literature are reviewed. The relation between guinea pig asthma and human asthma bronchiale is established. The underlying mechanism in both processes is the misdirected (aberrant) formation of anaphylactic or reaginic (IgE) antibody against innocuous antigens, leading at renewed exposure to the antigen to release of mast cell mediators (such as histamine, SRS-A, eosinotactic peptides, PAF), bronchoconstriction, vasodilation and eosinophilic inflammation at the sites of antigen-antibody reactions in the tissues of the respiratory organs.

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Section: IIsupporting

confidence: 72%

Section: Imentioning

confidence: 99%

Section: IVmentioning

confidence: 99%

Section: IVmentioning

confidence: 99%

See 2 more Smart Citations

Experimental Asthma in Guinea Pigs Revisited

Kallós¹,

Kallós²

1984

Int Arch Allergy Immunol

101

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“…Because of their complexity, methods for quantitation of anaphylaxis in large hypersensitive animals ( 1 ) are not readily adapted for the mouse. Consequently, the occurrence of anaphylaxis in mice is customarily determined using death as end point.…”

mentioning

confidence: 99%

Hematocrit Change as Indication of Anaphylactic Shock in the Mouse

Fulton

Harris

Craft

1957

Experimental Biology and Medicine

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Because of their complexity, methods for quantitation of anaphylaxis in large hypersensitive animals ( 1 ) are not readily adapted for the mouse. Consequently, the occurrence of anaphylaxis in mice is customarily determined using death as end point. Attempts have been made to estimate sublethal anaphylaxis in mice by observing the appearance of certain gross signs (ie., cyanosis and convulsions). Because of their subjectivity, these observations are inadequate. Kind ( 2) found that the rectal temperatures of mice surviving anaphylactic shock were markedly decreased.In our laboratory we consistently observed that hemoconcentration occurs in mice undergoing anaphylaxis. We observed signs of anaphylaxis similar to those described by Weiser et al. (3) . Immediately after challenge most of the mice became quiet and huddled in one position. The fur became ruffled and there was mild lacrimation. There soon followed partial paralysis of the limbs and marked cyanosis of the limbs, tail, and snout. Many animals experienced brief but violent convulsions separated by short periods of complete prostration. Nearly all mice which underwent convulsions died within 30 minutes. Nelson et al.(4) reported that the hematocrit of pooled mouse blood was markedly increased following fatal anaphylactic shock. The present study was designed to determine whether or not hematocrit change might be a suitable criterion for determination of anaphylaxis in mice.Methods. Sensitization and challenge. Fol-lowing randomization, female Swiss-Webster mice (22-27 g) were sensitized with 2 IP injections of 0.25 ml of antigen administered 4 days apart. Twelve days after receipt of the initial sensitizing injection the animals were challenged with an injection in the caudal vein of 0.25 ml of antigen. Two antigen preparations were employed: 1 ) alum precipitated bovine serum albumin* with Hemophilus pertussis vaccinet (APBA + HPV), each 0.25 ml containing 1 mg of bovine serum albumin suspended in 2.576 alum (pH 6.5 to 7.5) with lo9 to 1O1O H . pertussis vaccine cells; and, 2 ) the same as above except that H . pertussis vaccine was not included. This antigen preparation is referred to as APBA. A single challenge antigen preparation, bovine serum albumin with H . pertussis vaccine (BSA + HPV) was used. Each 0.25 ml of this preparation contained 4 mg of bovine albumin and 109 to 1010 cells H . pertussis vaccine. Controls included groups of mice sensitized with one or the other of the 2 sensitizing antigen preparations and challenged with saline, mice injected with saline instead of sensitizing antigen preparation and challenged with BSA + HPV, and groups given saline instead of both sensitization and challenge. Hematocrit determination: A modification of the microhematocrit method described by McGovern et al.( 5) was employed. Four-tenths mm I D microprecipitin capillary *Bovine serum albu,min, 3076, sterile, for use as t Pertussis vaccine (whooping cough bacterin), diluent in Rh testing. h o u r Labs. Wyeth Labs.

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The theoretical and experimental application of a conditioning model to a consideration of bronchial asthma in man

Franks¹,

Leigh²

1959

Journal of Psychosomatic Research

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New Contributions to Experimental Asthma

Cited by 7 publications

References 0 publications

Experimental Asthma in Guinea Pigs Revisited

Experimental Asthma in Guinea Pigs Revisited

Hematocrit Change as Indication of Anaphylactic Shock in the Mouse

The theoretical and experimental application of a conditioning model to a consideration of bronchial asthma in man

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