New cost effective design of PCR heating cycler system using Peltier plate without the conventional heating block

Nasser, Gamal Abdel; Abdel-Mawgood, Ahmed L.; Abouelsoud, A. A.; Mohamed, Hisham; Umezu, Shinjiro; El-Bab, Ahmed M. R. Fath

doi:10.1007/s12206-021-0646-5

Cited by 23 publications

(12 citation statements)

References 51 publications

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“…BSA weight (1 µg/ µl) dissolved in freshly prepared phosphate buffer saline (PBS)solution (pH 7.4) 24 . PCR cycling was performed in a Lab cycler (SensoQuest GmbH, Germany) with the following parameters: one cycle of 4 min at 94°C, followed by 35 cycles of denaturation for 1 min at 94°C, annealing for 30 s at 58°C, extension for 2 min at 72°C and nal extension at 72°C for 5 minutes 25 . The reaction mixture was added to a PCR tube containing a piece of ER and ER coated with BSA to test its effect on the PCR test e ciency.…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

Fabrication of BA-doped ER-based microfluidics using CO 2 laser ablation: an impact of doping ER with boric acid

Mansour

Soliman

El-Bab

et al. 2023

Preprint

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This study investigated the thermal properties of epoxy resin by adding boric acid with different concentrations. The chemical structural and optical properties of the neat epoxy resin and the epoxy resin filled with different concentrations of boric acid were characterized by FTIR and spectrophotometer. TGA, DSC, and DTA investigate the thermal characteristics. Moreover, a specific migration test was accomplished to quantify potential migrants by GC–MS to prove that the prepared samples would not release unreacted monomers or boric acid to the biological solution test, which caused inhibition of or interfered with the sensitive biological reactions. By investigating the impact of this process on microchannels' dimensions and quality, a laser technique using CO2 laser was used for engraving into a transparent epoxy resin chip. The resulting microchannels were characterized using 3D laser microscopy. The outcomes of this study showed a reduction in the surface roughness and negligible bulge heights in the laser-ablated microchannels. This study validated the addition of boric acid to enhance the thermal properties of the cured epoxy resin, which led to better microchannel quality.

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Section: Polymerase Chain Reactionmentioning

confidence: 99%

Fabrication of BA-doped ER-based microfluidics using CO 2 laser ablation: an impact of doping ER with boric acid

Mansour

Soliman

El-Bab

et al. 2023

Preprint

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“…Interestingly, the Peltier element reported in that work did not include the heat distribution plate commonly found in these elements acting as a contact thermal resistance to improve the spatial uniformity of the temperature field and prevent abrupt temperature changes. Therefore, heating and cooling rates increased due to the elimination of this feature [59]. In a different contribution, a Peltier element was attached to one side of a PMMA tube wall, covering an area of 10 × 30 mm 2 where the temperature varied from 22 to 36 • C. It was possible to generate a constant temperature gradient therein by fixing a specific power value for the Peltier element and ensuring a constant air flow (inside the PMMA tube) through a pressure reducer [25].…”

Section: Peltier Elementsmentioning

confidence: 99%

“…Temperature control in microfluidics has been explored in applications, including cell culture [ 49 , 50 , 51 , 52 ], cell imaging [ 53 ], cell analysis [ 54 , 55 , 56 ], PCR [ 22 , 57 – 60 ], nucleic acid amplification test assays [ 61 ], microreactors [ 62 ], transparent electrodes [ 63 , 64 ], enzyme‐linked immunosorbent assay [ 44 ], biochemical synthesis [ 65 ], particle trapping [ 66 ], and microelectronic device cooling [ 67 ], among others [ 68 , 69 ]. For this, the use of microheaters [ 52 , 55 – 57 , 62 , 70 , 71 ], Peltier elements [ 25 , 58 , 59 , 72 ], two‐phase materials [ 73 ], laser beams [ 74 ], and cement power resistors [ 60 ] has been reported.…”

Section: Introductionmentioning

confidence: 99%

Recent advances and challenges in temperature monitoring and control in microfluidic devices

et al. 2022

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Temperature is a critical—yet sometimes overlooked—parameter in microfluidics. Microfluidic devices can experience heating inside their channels during operation due to underlying physicochemical phenomena occurring therein. Such heating, whether required or not, must be monitored to ensure adequate device operation. Therefore, different techniques have been developed to measure and control temperature in microfluidic devices. In this contribution, the operating principles and applications of these techniques are reviewed. Temperature‐monitoring instruments revised herein include thermocouples, thermistors, and custom‐built temperature sensors. Of these, thermocouples exhibit the widest operating range; thermistors feature the highest accuracy; and custom‐built temperature sensors demonstrate the best transduction. On the other hand, temperature control methods can be classified as external‐ or integrated‐methods. Within the external methods, microheaters are shown to be the most adequate when working with biological samples, whereas Peltier elements are most useful in applications that require the development of temperature gradients. In contrast, integrated methods are based on chemical and physical properties, structural arrangements, which are characterized by their low fabrication cost and a wide range of applications. The potential integration of these platforms with the Internet of Things technology is discussed as a potential new trend in the field.

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“…The parameter temperature is the fundamental element for the PCR system in biochemical reactions . The optimum target temperature was usually dependent on the reaction situation or the physical characteristics of the molecules in a particular solvent and so forth.…”

Section: Introductionmentioning

confidence: 99%

“…15,16 The parameter temperature is the fundamental element for the PCR system in biochemical reactions. 17 The optimum target temperature was usually dependent on the reaction situation or the physical characteristics of the molecules in a particular solvent and so forth. The precise control of the sample temperature was critical during the sample reaction and separation process in a PCR system.…”

Section: Introductionmentioning

confidence: 99%

Construction of Very Low-Cost Loop Polymerase Chain Reaction System Based on Proportional-Integral-Derivative Temperature Control Optimization Algorithm and Its Application in Gene Detection

et al. 2022

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Real-time polymerase chain reaction (PCR) technology is essential in nucleic acid detection and point-of-care testing (POCT). However, nowadays, the classical qPCR instrument has the deficiency of its bulky volume, high cost, and inconvenience to use; hence, a low-cost and easy-to-use PCR equipment was thus developed consisting of a hardware subsystem as well as a software subsystem based on an improved proportional-integral-derivative (PID) system. The proposed system not only could hold self-setting reaction cycles of temperature rising and falling automatically but also the temperature during the constant temperature stage was regulated steady based on improved temperature control algorithm, which proved its great effect compared with the reaction temperature derived from an infrared thermal imaging camera. The experimental results in gene detection research also could indicate its applicability and stability of our developed PCR system by using the amplification curve analysis, the melting curve analysis, and agarose gel electrophoresis analysis compared with the commercial PCR instrument, which illustrates the great potential application value of the proposed PCR system.

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New cost effective design of PCR heating cycler system using Peltier plate without the conventional heating block

Cited by 23 publications

References 51 publications

Fabrication of BA-doped ER-based microfluidics using CO 2 laser ablation: an impact of doping ER with boric acid

Fabrication of BA-doped ER-based microfluidics using CO 2 laser ablation: an impact of doping ER with boric acid

Recent advances and challenges in temperature monitoring and control in microfluidic devices

Construction of Very Low-Cost Loop Polymerase Chain Reaction System Based on Proportional-Integral-Derivative Temperature Control Optimization Algorithm and Its Application in Gene Detection

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