New Delhi Metallo-β-lactamase and other mechanisms of carbapenemases among Enterobacteriaceae in rural South India

Sekar, Ramalingam; Srivani, Seetharaman; Kalyanaraman, Narayanan; Thenmozhi, Pandiyan; Amudhan, Murugesan; Lallitha, Sivathanu; Mythreyee, Manoharan

doi:10.1016/j.jgar.2019.05.028

Cited by 13 publications

(16 citation statements)

References 30 publications

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“…This agrees with the previous studies reporting that K. pneumoniae and E. coli were predominant CRE among Enterobacteriaceae. 4,[29][30][31] We found a high rate of antimicrobial resistance. In addition, these CRE pose the greatest risk to the public health because of their high prevalence, high potential for causing a wide range of clinical infections, co-resistance to Beta-lactam as well as other antimicrobial agents (such as aminoglycosides and fluoroquinolones).…”

Section: Discussionmentioning

confidence: 82%

“…In addition, these CRE pose the greatest risk to the public health because of their high prevalence, high potential for causing a wide range of clinical infections, co-resistance to Beta-lactam as well as other antimicrobial agents (such as aminoglycosides and fluoroquinolones). 4,29 Understanding the antibiotic susceptibility patterns of CRE is important for antibiotic therapy of infections. In our study, amikacin showed the highest susceptibility rate (85%).…”

Section: Discussionmentioning

confidence: 99%

“…Other studies confirmed that the most frequent non-susceptible carbapenems were meropenem and imipenem. 4,31 Therefore, the CLSI and EUCAST recommend the MIC test for routine clinical isolates. 16,36 The carbapenem-hydrolyzing activity of class C-beta lactamases has been reported.…”

Section: Discussionmentioning

confidence: 99%

“…3 Emergence and dissemination of carbapenem resistance have been increasingly reported across the world which limits the usage of carbapenems. 4 Carbapenemresistant Enterobacteriaceae (CRE) is a significant clinical and public health concern. 5 Considering their transmissibility and limited treatment options against CRE, the Centers for Disease Control and Prevention (CDC) considers CRE as specially dangerous.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Resistance Mechanisms in Carbapenem-Resistant Enterobacteriaceae

Alizadeh¹,

Rezaee²,

Kafil³

et al. 2020

IDR

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Background: Carbapenem-resistant Enterobacteriaceae (CRE) is a major concern leading to morbidity and mortality in the world. CRE often is becoming a cause of therapeutic failure in both hospital and community-acquired infections. Aim: This study aimed to investigate the resistance mechanisms of CRE by phenotypic and molecular methods. Materials and Methods: Sixty CRE (50 Klebsiella pneumoniae, 6 Escherichia coli, and 4 Enterobacter spp.) were isolated from October 2018 to June 2019. Antimicrobial susceptibility testing was carried out using phenotypic methods. The carbapenem resistance mechanisms including efflux pump hyperexpression, AmpC overproduction, carbapenemase genes, and deficiency in OmpK35 and OmpK36 were determined by phenotypic and molecular methods, respectively. Results: Sixty CRE (50 Klebsiella pneumoniae, 6 Escherichia coli, and 4 Enterobacter spp.) were isolated from October 2018 to June 2019. Amikacin was found to be the most effective drug against CRE isolates. All isolates were resistant to imipenem and meropenem by the micro-broth dilution. AmpC overproduction was observed in all Enterobacter spp. and three K. pneumoniae isolates. No efflux pump activity was found. Carba NP test and Modified Hodge Test could find carbapenemase in 59 (98%) isolates and 57 (95%) isolates, respectively. The most common carbapenemase gene was bla OXA-48-like (72.8%) followed by bla NDM (50.8%), bla IMP (18.6%), bla VIM (11.8%), and bla KPC (6.7%). The ompK35 and ompK36 genes were not detected in 10 and 7 K. pneumoniae isolates, respectively. Conclusion: The amikacin is considered as a very efficient antibiotic for the treatment of CRE isolates in our region. Carbapenemase production and overproduction of AmpC are the main carbapenem resistance mechanisms in CRE isolates. Finally, Carba NP test is a rapid and reliable test for early detection of carbapenemase-producing isolates.

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Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of Resistance Mechanisms in Carbapenem-Resistant Enterobacteriaceae

Alizadeh¹,

Rezaee²,

Kafil³

et al. 2020

IDR

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“…Several studies have demonstrated a predominance of metallo-beta-lactamases in India. 18,[23][24][25] 3 isolates of Escherichia coli produced Serine Beta-lactamase enzyme. Our findings were comparable to studies conducted in Pune 15 and.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of various Beta-lactamases in Enterobacteriaceae in a tertiary care hospital in South India: A Cross-sectional study

S¹,

Kaup

2021

IJMMTD

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Antimicrobial resistance among bacteria has constantly increased over the years with emergence of novel resistance mechanism among these versatile organisms. Identifying the resistance pattern of the isolates both regionally and globally is essential for the institution of appropriate antimicrobial therapy. This study was conducted to assess the prevalence of beta-Lactamase enzymes among species of Enterobacteriaceae.312 consecutive isolates belonging to the family of Enterobacteriaceae were included in the study conducted over a period of one year. After preliminary antibiotic susceptibility testing using standard guidelines, production of various beta-Lactamase enzymes was assessed by phenotypic methods.22.76% of the isolates were found to be Extended-Spectrum Beta-Lactamase producers, 37.82% were AmpC Beta-Lactamase producers and 7.37% of the isolates produced the Carbapenemase enzyme.Our study estimates the prevalence of various beta-Lactamase enzymes in isolates of Enterobacteriaceae in a tertiary care centre. The increase in production of these enzymes among bacteria necessitates the implementation of strict adherence to antibiotic policy and infection control measures.

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Comparative evaluation of phenotypic and genotypic methods for the rapid and cost-effective detection of carbapenemases in extensively drug resistant Klebsiella pneumoniae

Kundu¹,

Rathore²,

Kanaujia³

et al. 2023

Indian Journal of Medical Microbiology

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New Delhi Metallo-β-lactamase and other mechanisms of carbapenemases among Enterobacteriaceae in rural South India

Cited by 13 publications

References 30 publications

<p>Evaluation of Resistance Mechanisms in Carbapenem-Resistant <em>Enterobacteriaceae</em></p>

<p>Evaluation of Resistance Mechanisms in Carbapenem-Resistant <em>Enterobacteriaceae</em></p>

Prevalence of various Beta-lactamases in Enterobacteriaceae in a tertiary care hospital in South India: A Cross-sectional study

Comparative evaluation of phenotypic and genotypic methods for the rapid and cost-effective detection of carbapenemases in extensively drug resistant Klebsiella pneumoniae

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