New Developments in Simulated Moving Bed Chromatography

Abstract

Chromatographic separation processes are widely used to isolate and purify value added products. Most frequently, such separation processes are performed exploiting the principles of injecting samples of the feed mixture in a repetitive periodic manner and collecting the target products batchwise. In the early 1960s, an alternative operation principle based on using several columns connected in series and exploiting a continuous countercurrent movement between the mobile and stationary phases initiated significant improvements in performing specific separation processes in the petrochemical and sugar industries. In the last decade, the so-called simulated moving bed (SMB) chromatography has rapidly entered the pharmaceutical industry. In particular enantioseparations using chiral stationary phases have become a strong driving force to develop more sophisticated concepts of continuous chromatography. Currently, the growing interest in efficient methods for the downstream processing of biomolecules enhances interest in SMB processes. This paper gives a brief overview of the new suggestions and trends. © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim [accessed July 4, 2008

Countercurrent tangential chromatography for large‐scale protein purification

“…Although some of these limitations can be overcome by using chromatographic monoliths (Jungbauer and Hahn, 2004) or membrane adsorbers (Zhou and Tressel, 2006), these technologies have yet to be employed in a significant way in large-scale bioprocessing (van Reis and Zydney, 2007). There has also been renewed interest in the use of simulated moving bed chromatography (Seidel-Morgenstern et al, 2008) and expanded bed adsorption (Hubbuch et al, 2005) for bioprocessing, but there have been very few large-scale applications for protein purification. Non-chromatographic separations like precipitation and crystallization have also been considered for large-scale production of biotherapeutics (Shukla and Thommes, 2010;Thommes and Etzel, 2007), but none of these has yet been adopted in any significant way.…”

Advanced Reprocessing – The Potential for Continuous Chromatographic Separations

“…If the balance between mobile and stationary phase flow rates is balanced correctly, components with higher affinity for the mobile phase would be carried further with this than the stationary phase. Movement of the mobile phase is inherently plagued with hydrodynamic challenges; to overcome them a certain number of fixed beds are connected in series to form a closed loop, and the counter-current movement of the solid and liquid phase is simulated by periodically shifting the fluid inlets and outlets in the direction of the fluid flow i.e., simulated moving bed (SMB) [11]. An example of a laboratory SMB reactor is shown in Figure 2.…”

Verfahren zur Enantiomerentrennung

“…B. Lit. [267][268][269][270]), die auch die Anwendung überkritischer Flüssigkeiten als mobile Phase beinhalten. [271,272] Die Verfügbarkeit dieser Varianten erweiterte das Anwendungsspektrum [273] und führte bereits zu den ersten erfolgreichen Reinigungen von Biomolekülen mittels kontinuierlicher Chromatographie.…”