New diffractometric equations and data processing algorithm for laser ektacytometry of red blood cells

Nikitin, S. M.; Ustinov, V.D.; Yurchuk, Y.S.; Луговцов, А.Е.; Lin, M. D.; Priezzhev, Alexander V.

doi:10.1016/j.jqsrt.2016.02.024

Cited by 10 publications

(3 citation statements)

References 20 publications

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“…On the eve of the jubilee, Alexander was awarded the MSU First Prise for the scientific publications in the frames of the MSU Programme of Development (2016). This award characterises well the scientific J of Biomedical Photonics & Eng 3 112 Apr 2017 © JBPE 010102-4 achievements, wide scope and prospects of the scientific field, developed by Alexander at present time, it is sufficient just to read the titles of some papers for which this prise was awarded [7][8][9][10][11][12][13][14][15][16][17][18].…”

Section: Saratov Fall Meeting-2016mentioning

confidence: 88%

To the Jubilee of Professor Alexander Priezzhev

Romanovsky

Tuchin

2017

JBPE

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Section: Saratov Fall Meeting-2016mentioning

confidence: 88%

To the Jubilee of Professor Alexander Priezzhev

Romanovsky

Tuchin

2017

JBPE

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“…Under high shear stresses, the RBCs are elongated, taking shapes close to ellipsoidal and so does the average diffraction pattern, which is a result of the superposition of thousands of diffraction patterns from individual cells. Analyzing the degree of ellipsoid elongation of diffraction pattern allows for measuring the average deformability of the RBC population as a function of shear stress in the range from 0.5 to 20 Pa in terms of elongation index (EI) (Groner et al, 1980; Shin et al, 2005; Nikitin et al, 2016). The dependence of EI on shear stress is called the deformability curve.…”

Section: Methodsmentioning

confidence: 99%

The Effects of Different Signaling Pathways in Adenylyl Cyclase Stimulation on Red Blood Cells Deformability

et al. 2019

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Signaling pathways of red blood cells’ (RBCs) micromechanics regulation, which are responsible for maintaining microcirculation, constitute an important property of RBC physiology. Selective control over these processes may serve as an indispensable tool for correction of hemorheological disorders, which accompany a number of systemic diseases (diabetes mellitus I&II, arterial hypertension, malaria, etc.). Activation of certain pathways involving adenylyl cyclase may provide fast adaptive regulation of RBC deformability (RBC-D). However the specific molecular conditions of intracellular signal transduction in mediating RBC microrheological properties at adenylyl cyclase stimulation remain unclear. In this paper, we present the results of the in vitro study of the effects of different signaling pathways in adenylyl cyclase stimulation on RBC-D. We studied (1) the direct stimulation of adenylyl cyclase with forskolin; (2) non-selective adrenoreceptor stimulation with epinephrine; (3) β2-adrenoreceptor agonist metaproterenol; (4) membrane-permeable analog of cAMP (dibutyryl-cAMP). Using laser ektacytometry, we observed a concentration-dependent increase in RBC-D for all studied effectors. The EC50 values for each substance were estimated to be in the range of 1–100 μM depending on the shear stress applied to the RBC suspension. The results can serve as an evidence of adenylyl cyclase signaling cascade involvement in the regulation of RBC micromechanical properties presenting a complex molecular pathway for fast increase of microcirculation efficiency in case of corresponding physiologic metabolic demands of the organism, e.g., during stress or physical activity. Further studies of this molecular system will reveal new knowledge which may improve the quality of medical treatment of hemorheological disorders.

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“…A sample with a small overall fraction of extremely rigid RBCs could render a similar elongation curve as a sample with a high fraction of moderately stiff RBCs. Previous studies have investigated how laser diffractions in ektacytometry differ based on non-homogeneous mixtures of RBC deformability 29 . However, to date, there is no established method for isolating and directly characterizing the rigidity of the HbS RBC population in patient blood.…”

Section: Introductionmentioning

confidence: 99%

Characterizing bulk rigidity of rigid red blood cell populations in sickle-cell disease patients

Gutiérrez

Shamoun

Seu

et al. 2021

Sci Rep

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In this work, we utilized a parameterization model of ektacytometry to quantify the bulk rigidity of the rigid red blood cell (RBC) population in sickle cell disease (SCD) patients. Current ektacytometry techniques implement laser diffraction viscometry to estimate the RBC deformability in a whole blood sample. However, the diffraction measurement is an average of all cells present in the measured sample. By coupling an existing parameterization model of ektacytometry to an artificially rigid RBC model, we formulated an innovative system for estimating the average rigidity of the rigid RBC population in SCD blood. We demonstrated that this method could more accurately determine the bulk stiffness of the rigid RBC populations. This information could potentially help develop the ektacytometry technique as a tool for assessing disease severity in SCD patients, offering novel insights into the disease pathology and treatment.

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New diffractometric equations and data processing algorithm for laser ektacytometry of red blood cells

Cited by 10 publications

References 20 publications

To the Jubilee of Professor Alexander Priezzhev

To the Jubilee of Professor Alexander Priezzhev

The Effects of Different Signaling Pathways in Adenylyl Cyclase Stimulation on Red Blood Cells Deformability

Characterizing bulk rigidity of rigid red blood cell populations in sickle-cell disease patients

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