New Enzyme Immunoassays for Sensitive Detection of Circulating
            <i>Candida albicans</i>
            Mannan and Antimannan Antibodies: Useful Combined Test for Diagnosis of Systemic Candidiasis

Sendid, Boualem; Tabouret, Marc; Poirot, J. L.; Mathieu, Daniel; Fruit, J.; Poulain, Daniel

doi:10.1128/jcm.37.5.1510-1517.1999

Cited by 191 publications

(78 citation statements)

References 71 publications

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“…However, combining the M ELISA with a Platelia ELISA for M antibody (positive >10 U) raised the sensitivity to 80% while retaining specificity. Sensitivity and specificity for the M antibody ELISA alone were 53% and 94%, respectively (Sendid et al, 1999).…”

Section: Mannan and Anti-mannan Detectionmentioning

confidence: 99%

Advances in the molecular and serological diagnosis of invasive fungal infection in haemato‐oncology patients

McLintock

Jones

2004

Br J Haematol

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Summary Current laboratory diagnostic methods for invasive fungal infection (IFI) in haemato‐oncology patients are insensitive, resulting in late diagnosis and contributing to high mortality. In recent years, progress has been made in the development and evaluation of sensitive sero‐diagnostic assays, including detection of genomic DNA sequences and fungal antigens, which aid in a rapid, early diagnosis of IFI. The sensitivity and specificity of the assays vary considerably between studies, highlighting the need to correlate serological results with conventional laboratory tests and clinical or radiological findings. As part of management protocols, these assays may help to confirm the diagnosis of suspected IFI; however, the impact on mortality from IFI may be greatest when they are used to screen high‐risk patients. Persistently positive screening results could direct early aggressive antifungal therapy, guided further by radiological and microbiological findings combined with regular clinical review, while the excellent negative predictive value may allow treatment to be withheld in patients with antibiotic resistant neutropenic fever but no other signs of IFI. However, this pre‐emptive approach requires evaluation in prospective randomized trials.

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Section: Mannan and Anti-mannan Detectionmentioning

confidence: 99%

Advances in the molecular and serological diagnosis of invasive fungal infection in haemato‐oncology patients

McLintock

Jones

2004

Br J Haematol

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“…While individual sensitivity of these tests is low (<50%), the combined detection increased the sensitivity (60-89%). 38,44,45…”

Section: Detection Of Candida Mannan and Anti-mannan Antibodiesmentioning

confidence: 99%

Current diagnostic approaches to invasive candidiasis in critical care settings

Pemán

Zaragoza²

2010

Mycoses

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For the specialist, the management of invasive candidiasis infections, from diagnosis to selection of the therapeutic protocol, is often a challenge. Although early diagnosis and treatment are associated with a better prognosis, apart from cases with positive blood cultures or fluid/tissue biopsy, diagnosis is neither sensitive nor specific, relying on many different factors, clinical and laboratory findings but there is certainly a need for the specific markers in this disease. Recently, new serodiagnostic assays as Candida albicans germ-tube antibodies or (1,3)-beta-D-glucan detection and molecular techniques for the detection of fungal-specific DNA have been developed with controversial results in critical care setting. One of the main features in diagnosis is the evaluation of risk factor for infection, which will identify patients in need of preemptive or empirical treatment. Clinical scores were built from those risk factors. For these reasons, an approach to the new diagnosis tools in the clinical mycology laboratory and an analysis of the new prediction rules and its application situations has been made. Currently, the combination of prediction rules and non-culture microbiological tools could be the clue for improving the diagnosis and prognosis of invasive fungal infections in critically ill patients.

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“…When 80 pg ml )1 is used as the cutoff, sensitivity is 64% and specificity is 92%. [4][5][6][7][8] False-positive BG results can occur with bacteremia, haemodialysis, blood transfusions, human serum albumin, haemolysed specimen and contact with gauze. [9][10][11][12][13] Performance characteristics of serum BG assays in burn patients are not known.…”

Section: Introductionmentioning

confidence: 99%

Early serum (1→3)‐β‐D‐glucan levels in patients with burn injury

Shupp

Petraitienė

Jaskille

et al. 2011

Mycoses

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Serum (1→3)-β-D-glucan (BG) is increasingly used as diagnostic marker for invasive fungal infections. Exposure to gauze may lead to false-positive BG assays. The role of BG is unclear in thermally injured patients who frequently require extensive gauze coverage; therefore, we prospectively evaluated BG levels in burn-injured patients. Serum BG levels were measured in 18 burn patients immediately before application of the first dressing and 12 h after. Patients were stratified by extent of total body surface area (TBSA) requiring gauze coverage: <20%, 20-39%, 40-60% and >60%. BG levels were obtained from patients with non-burn trauma as controls. BG results were positive (>80 pg ml⁻¹) in 9/18 (50%) patients at baseline and in 8/18 (44%) 12 h after application of the first dressing. BG levels were positive in 1/5 (20%) of patients with <20% TBSA requiring gauze and in 10/13 (77%) with ≥ 20% (P < 0.05). None of the control patients had positive BG at any time point and none of the patients had candidemia at baseline. Mean serum BG levels decreased (19.44 pg ml⁻¹) after gauze placement. False-positive serum BG elevations are common in this patient population. Positivity correlates with extent of TBSA injured, but is not impacted by the gauze itself.

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New Enzyme Immunoassays for Sensitive Detection of Circulating Candida albicans Mannan and Antimannan Antibodies: Useful Combined Test for Diagnosis of Systemic Candidiasis

Cited by 191 publications

References 71 publications

Advances in the molecular and serological diagnosis of invasive fungal infection in haemato‐oncology patients

Advances in the molecular and serological diagnosis of invasive fungal infection in haemato‐oncology patients

Current diagnostic approaches to invasive candidiasis in critical care settings

Early serum (1→3)‐β‐D‐glucan levels in patients with burn injury

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