New epiphytic sooty molds: Alloscorias syngonii (Readerielliopsidaceae) from Thailand

Haituk, Sukanya; Suwannarach, Nakarin; Hongsanan, Sinang; Senwanna, Chanokned; Cheewangkoon, Ratchadawan

doi:10.11646/phytotaxa.507.4.1

Cited by 4 publications

(3 citation statements)

References 23 publications

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“…The obtained genomic DNA was resuspended in 50 µL TE buffer and stored at −20 • C. Four loci sequences were amplified in an automated thermal cycler (Eppendorf AG, Germany). The primer pairs ITS5 and ITS4 were used for amplification of rDNA ITS regions [20], primers Bt2a (5 -GGT AAC CAA ATC GGT GCT TTC -3 ) and Bt2b (5 -ACC CTC AGT GTA GTG ACC CTT GGC -3 ) for the beta-tubulin gene (TUB) [21], primers 5F (5 -GAY MGW GAT CAY TTY GG -3 ) and 7CR (5 -CCC ATR GCT TGY TTR CCC AT -3 ) for the RNA polymerase II subunit B (RPB2) gene [22], and primers CMD5 (5 -CCG AGT ACA AGG ARG CCT TC -3 ) and CMD6 (5 -CCG ATR GAG GTC ATR ACG TGG -3 ) for the calmodulin gene [23]. The amplification was carried out in a 50 µL reaction volume containing 32.5 µL ddH 2 O, 5 µL 10 x PCR buffer, 4 µL of 2.5 mM dNTP, 2 µL of 10 µM of each primer, 4 µL template, and DNA 0.5 µL 5 U/µL Taq DNA polymerase (TaKaRa Bio Inc., Shiga, Japan).…”

Section: Phylogenetic Analysismentioning

confidence: 99%

Effect of Plant-Growth-Promoting Fungi on Eggplant (Solanum melongena L.) in New Reclamation Land

Yan

et al. 2021

Agriculture

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Land reclamation may expand the supply of usable land for food security. Immature soil is not suitable for plant growth and needs to be amended by the addition of organic matter and plant growth-promoting (PGP) microorganisms. However, the effects of different PGP fungi on plant growth in immature soil are largely unexplored. In order to obtain beneficial soil microorganisms with a good PGP ability in new reclamation land, 162 fungal isolates were isolated from different abandoned wastelands, four isolates of which were obtained in this study by the screening of P solubilization, siderophore production, and indole acetic acid (IAA) production. The result of this study revealed that isolate HZ123 had the highest ability to solubilize P and produce siderophores and IAA, followed by HZ23, HZ10, and HZ06. Based on the results of morphological and molecular analyses, isolate HZ06 was identified as Penicillium oxalicum, isolates HZ23 and HZ10 were identified as Aspergillus brunneoviolaceus, and isolate HZ123 was identified as Aspergillus tubingensis. Furthermore, the results of in vivo PGP assays demonstrated that isolate HZ123 has a minimal negative effect on the growth of eggplant; however, the other three isolates, particularly isolate HZ06, caused the greatest increase in eggplant biomasses. Overall, these results indicate that isolate HZ06 has great potential as a PGP fungus to develop biofertilizer for application in eggplant production in immature soil from new reclamation land.

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Section: Phylogenetic Analysismentioning

confidence: 99%

Effect of Plant-Growth-Promoting Fungi on Eggplant (Solanum melongena L.) in New Reclamation Land

Yan

et al. 2021

Agriculture

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“…The desired gene regions, partial large subunit nuclear rDNA (LSU) and internal transcribed spacer (ITS), were amplified using the primer pairs ITS5/ITS4 and LROR/LR5, respectively [21,22]. PCR was performed following the protocol described in Haituk et al [23]. The sequences were obtained from commercial sequence provider 1st BASE Company (Kembangan, Malaysia).…”

Section: Dna Extraction Amplification and Sequencingmentioning

confidence: 99%

“…The novel sequencing data generated in this work were analyzed using a nucleotide BLAST (BLASTn) search of the NCBI database (http://blast.ncbi.nlm.nih.gov/, accessed on 13 November 2021). The highly similar sequences were downloaded according to the BLASTn results and from recent publications [3,23,24]. The sequences were aligned using MAFFT v7 (RIMD, Osaka, Japan) [25].…”

Section: Phylogenetic Analysismentioning

confidence: 99%

Production of Non-Volatile Metabolites from Sooty Molds and Their Bio-Functionalities

et al. 2022

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In the current study, eleven sooty mold isolates were collected from different tropical host plants. The isolates were identified under Capnodium, Leptoxyphium and Trichomerium, based on morphology and phylogeny. For the secondary metabolite analysis, the isolates were grown on Potato Dextrose Broth (PDB). The well-grown mycelia were filtered and extracted over methanol (MeOH). The metabolites in the growth medium (or filtrate) were extracted over ethyl acetate (EtOAc). The antifungal activities of each crude extract were tested over Alternaria sp., Colletotrichum sp., Curvularia sp., Fusarium sp. and Pestalotiopsis sp. The metabolites were further tested for their total phenolic, flavonoid and protein content prior to their antioxidant and anti-fungal potential evaluation. The MeOH extracts of sooty molds were enriched with proteins and specifically inhibited Curvularia sp. The total phenolic content and 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) activity was largely recovered from the filtrate corresponding to the inhibition of Alternaria sp.; while the flavonoid and free radical reduction suggested a relative induction of growth of the Fusarium sp., Colletotrichum sp. and Pestalotiopsis sp. Hence, this study reveals the diversity of sooty molds in Thailand by a modern phylogenetic approach. Furthermore, the preliminary screening of the isolates reveals the potential of finding novel compounds and providing insights for the future research on secondary metabolites of bio-trophic fungi and their potential usage on sustainable agriculture.

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Recovery of Orange Peel Essential Oil from ‘Sai-Namphaung’ Tangerine Fruit Drop Biomass and Its Potential Use as Citrus Fruit Postharvest Diseases Control

et al. 2022

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In this study, we assessed the quality of essential oil recovered from fruit drop biomass and assessed its usefulness in preventing postharvest diseases in the tangerine ‘Sai-Namphaung’. Greening was the primary cause of the fruit drop, based on the enduring symptoms and occurrence of the disease in the area. Limonene, together with the presence of β-pinene and linalool, was discovered to be prevalent in essential oils of tangerine fruit peel, particularly that of ‘Sai-Namphaung’. Through isolation of citrus postharvest fungi, we were able to identify four genera which were later DNA sequenced using Internal Transcribed Spacer: ITS and subjected to Basic Local Alignment Search Tool (BLAST), with a high possibility (>98% similarity) of being Penicillium digitatum, Colletotrichum gloeosporioides, Fusarium sarcochrum and Geotrichum candidum. Essential oil from ‘Sai-Namphaung’ and ‘Fremont’ peel biomass positively inhibited green mold rot and citrus anthracnose caused by P. digitatum, C. gloeosporiodes, but were less effective than the commercial citrus oil and Zanthoxylum myriacanthum oil. This is the first evidence of ‘Sai-Namphaung’ postharvest diseases caused by these two fungi and their controls using citrus essential oil.

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New epiphytic sooty molds: Alloscorias syngonii (Readerielliopsidaceae) from Thailand

Cited by 4 publications

References 23 publications

Effect of Plant-Growth-Promoting Fungi on Eggplant (Solanum melongena L.) in New Reclamation Land

Effect of Plant-Growth-Promoting Fungi on Eggplant (Solanum melongena L.) in New Reclamation Land

Production of Non-Volatile Metabolites from Sooty Molds and Their Bio-Functionalities

Recovery of Orange Peel Essential Oil from ‘Sai-Namphaung’ Tangerine Fruit Drop Biomass and Its Potential Use as Citrus Fruit Postharvest Diseases Control

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