New generation of bactericidal silver nanoparticles against different antibiotic resistant Escherichia coli strains

Kotakadi, Venkata Subbaiah; Gaddam, Susmila Aparna; Venkata, Sucharitha K.; Gopal, D. V. R. Sai

doi:10.1007/s13204-014-0381-7

Cited by 45 publications

(24 citation statements)

References 46 publications

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“…5). The similar result has been observed with silver NPs synthesized with plant extracts [7,9] and fungal extract [29].…”

Section: Scanning Electron Microscopy (Sem) Analysissupporting

confidence: 86%

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An in-vitro Cytotoxic and Genotoxic Properties of Allmanda Cathartica L. Latex Green NPs on Human Peripheral Blood Mononuclear Cells

Nelaturi¹,

Sriramaiah²,

Nagaraj³

et al. 2017

Nano Biomedicine and Engineering

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Green synthesis of silver nanoparticles (NPs) by green route approaches has advantages over conventional methods. In green synthesis, we use eco-friendly plant extracts contain secondary metabolites and bioactive components, proteins that act as both reducing and capping agents, form stable and shape-controlled green silver nanoparticles. The current study deals with the synthesis of silver nanoparticles using the aqueous latex extract of Allamanda cathartica. The green silver nanoparticles are characterized by using different spectroscopic methods like ultra violet-visible spectroscopy (UV-Vis), Fourier transform-infrared spectroscopy (FTIR), transmission electron microscope (TEM), scanning electron microscope (SEM) and X-ray diffraction (XRD). Results indicated that the crystalline natured particles were spherical shaped with an average of 35 nm in size, and that the stability of silver nanoparticles was due to its high negative zeta potential of-27.6 mV. The current study also revealed that green silver nanoparticles had very good genotoxic and cytotoxic activity in peripheral blood mononuclear cells (PBMCs). Leukemia leads to the development of high numbers of white blood cells, which is one of the major types of cancers that affect children. Many of the chemicals used for the treatment produce remarkable side effects. To overcome this problem, we made an attempt to see the efficacy of latex green silver nanoparticle on peripheral blood mononuclear cells and deoxyribonucleic acid fragmentation, which leads to the development of future therapeutic drugs.

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“…5). The similar result has been observed with silver NPs synthesized with plant extracts [7,9] and fungal extract [29].…”

Section: Scanning Electron Microscopy (Sem) Analysissupporting

confidence: 86%

“…TEM images showed that the particle size ranged from 19-52 nm. The particles are spherical, rectangular, a few with striations, uniformly distributed without any aggregation [9].…”

Section: O R P H O L O G Y O F G R E E N S I L V E R N P S W I T H mentioning

confidence: 99%

An in-vitro Cytotoxic and Genotoxic Properties of Allmanda Cathartica L. Latex Green NPs on Human Peripheral Blood Mononuclear Cells

Nelaturi¹,

Sriramaiah²,

Nagaraj³

et al. 2017

Nano Biomedicine and Engineering

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“…Due to the presence of negative charge on the surface, agglomeration of Tb-AgNPs in aqueous solution was prevented, and long-term stability was also allowed. This clearly indicated that the nanoparticles were stable because the minimum charge required to stabilize the nanoparticles was +30 mV or −30 mV [40,[13][14]. The results revealed and concluded that the biosynthesized Tb-AgNPs with the fruit extract of Terminalia belarica were very stable in aqueous environment.…”

Section: Analysis Of Zeta Potential Of Green Tb-agnpsmentioning

confidence: 86%

“…So, green chemistry methods were found to be a good alternative for biosynthesis of metallic AgNPs; they were nonhazardous, simple, inexpensive and environment friendly. Green silver nanoparticles gained their potential in antibacterial, antifungal, anticancer and biomedical applications [9][10][11][12][13][14][15]. The plant extracts have more potential in the synthesis of metal nanoparticles as they contain polyphenols, alkaloids, fatty acids, proteins, flavonoids which help reducing and capping agents.…”

Section: Introductionmentioning

confidence: 99%

Multifunctional Silver Nanoparticles by Fruit Extract of Terminalia belarica and their Therapeutic Applications: A 3-in-1 System

Venkata¹,

Gaddam²,

Kotakadi³

et al. 2018

Nano BioMed ENG

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The use of nanoparticles in treating dreadful diseases like cancer is the emerging field of research in cancer therapy. In the present investigation, the green biosynthesis of silver nanoparticles (AgNPs) with aqueous fruit extract of Terminalia belarica has been carried out, and ultraviolet-visible spectroscopy (UV-Vis) analysis was done which revealed an intense surface plasmon resonance (SPR) band at 430 nm, thus confirming the formation of Tb-AgNPs. The AgNPs were further characterized by Fourier transform-infrared spectroscopy (FTIR), showing the reduction of silver nitrate into Tb-AgNPs by the reduction of different functional groups such as hydroxyl, phenols, stretch of aldehydes, alkenes and aromatics. Transmission electron microscopy (TEM) and diffraction light scattering (DLS) study showed that the nanoparticles were round in shape with an average size of 46.5 nm. The atomic force microscopy (AFM) analysis also revealed similar results to facilitate that the AgNPs were round in shape, and the size was calculated by Z-coloration method. Furthermore, the X-ray diffraction (XRD) data confirmed that Tb-AgNPs were crystalline with face centered cubic (fcc) structure and were very stable with −29.1 mV Zeta potential. Tb-AgNPs showed efficient free radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl, hydrogen peroxide and nitric oxide. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) method was the best among the three antioxidant methods with the half maximal inhibitory concentration (IC 50) value of 47.25 ± 0.17. Tb-AgNPs also showed superior and efficient antibacterial activity greater than the control antibiotic, and showed effective anti-proliferative and cytotoxic effect on human breast cancer cells with IC 50 value of 73.18 µg/mL. The biosynthesized Tb-AgNPs with multifunctional properties could be employed as a source for the exploration of novel therapeutic antioxidant, antibacterial and anticancer agent.

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“…AgNPs cause toxicity to the bacterial cell constituents, damage to mitochondria in particular, and result in depletion of ATP which enhances the production of ROS. Then there was a double impact of both ROS and AgNPs on the nucleus of Epi-fluorescent microscopic images of mixed bacterial species isolated from drinking water PVC pipeline a initial-2 h, b 2-4 h, c 4-6 h, d 6-7 h and after during interfacial contact studies in the presence of nanoparticles loaded in nutrient agar medium bacterial cell leads to oxidation of DNA causing the death of the bacterial cell (Venkata Subbaiah et al 2014). Similar results were observed by previous investigators for ZnO nanoparticles (Raghupathi et al 2011;Sivakumar et al 2010).…”

Section: Antibacterial Activitymentioning

confidence: 99%

Antimicrobial kinetics of Alstonia scholaris bark extract-mediated AgNPs

et al. 2015

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Nanobiotechnology is considered as one of the important branches of nanotechnology, and research on synthesis of nanoscale materials, silver in particular, using plant and plant parts has been progressing rapidly. Herein, we used bark extract of Alstonia scholaris one of the most important medicinal plants to synthesize silver nanoparticles (AgNPs) which exhibited excellent antimicrobial properties against biofilm formed in drinking water PVC pipes. The biosynthesis of silver nanoparticles was done by treating 90 mL of 1 mM AgNO 3 aqueous solution with 10 mL of 5 % bark extract. As-prepared silver nanoparticles were characterized using the biophysical techniques such as UV-Vis spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, transmission electron microscopy, and dynamic light scattering for the measurement of hydrodynamic diameter and zeta potential. The kinetics of the antimicrobial activity against PVC biofilm of prepared silver nanoparticles were done using comparative solution suspension time-killing assessments and which are evidenced in Epi-fluorescent microscopic observations.

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New generation of bactericidal silver nanoparticles against different antibiotic resistant Escherichia coli strains

Cited by 45 publications

References 46 publications

An in-vitro Cytotoxic and Genotoxic Properties of Allmanda Cathartica L. Latex Green NPs on Human Peripheral Blood Mononuclear Cells

An in-vitro Cytotoxic and Genotoxic Properties of Allmanda Cathartica L. Latex Green NPs on Human Peripheral Blood Mononuclear Cells

Multifunctional Silver Nanoparticles by Fruit Extract of Terminalia belarica and their Therapeutic Applications: A 3-in-1 System

Antimicrobial kinetics of Alstonia scholaris bark extract-mediated AgNPs

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