New Hemoglobin in Normal Adult Blood

Tests of Glycemia in Diabetes

“…As early as 1955, investigators noted that adult human hemoglobin was heterogeneous (95,96). In 1958, Allen et al (97) reported that with cation-exchange chromatography human hemoglobin could be separated into at least three minor components that had more negative charges than HbA.…”

Is There a Relationship between Mean Blood Glucose and Glycated Hemoglobin?

“…3 Since 1955, laboratory methods were established to detect hemoglobin variants and "minor components" in human blood and were named HbA1a, HbA1b, and HbA1c, in order of their elution from a chromatography column. 4,5 Their relation to diabetes was appreciated much later when these minor hemoglobins were detected in diabetic patients 6,7 as well as in nondiabetic adults in a proportion of 1-4%. 8 Structural studies later established that the hemoglobin found in patients with diabetes was indeed identical to HbA1c.…”

Hemoglobin Hasharon in a Premature Infant with Hemolytic Anemia

“…Electrophoresis was performed on starch gels at pH 8.3 in Tris-EDTA-borate buffer. The proportions of the hemoglobins were determined by starch block electrophoresis using Verona1 buffer, pH 8.6 (14,15). Alkali-resistant hemoglobin was determined with standard techniques (14,27).…”