New, high-sensitivity high-performance liquid chromatographic method for the determination of acyclovir in human plasma, using fluorometric detection

Development and validation of a new reversed-phase ion pairing liquid chromatographic method with fluorescence detection for penciclovir analysis in plasma and aqueous humor

“…Conventional liquid-liquid extraction (LLE) could not be used since penciclovir is very polar (log P = −2.03) [47]. Direct injection after proteins precipitation with either an organic solvent or an acid was described for penciclovir related compounds such as aciclovir and ganciclovir [14][15][16][17][18][19][20][21][22][23][24][25][26][27]. However, injection of strongly acid solutions reduces analytical column lifetime, and leads to numerous late-eluting peaks disturbing the analytical procedure [22].…”

Determination of acyclovir in human serum by high-performance liquid chromatography using liquid–liquid extraction and its application in pharmacokinetic studies

“…As these solvents cannot easily be evaporated, dilution of the samples reduces the method sensitivity. Different limits of quantification (LOQ) ranging from 10 to 200 ng/mL of serum have been reported in published methods, however; LOQ of less than 50 ng/mL has been achieved in these methods by either increasing the injection volume [6,8,9,11,13] and/or application of highly acidic mobile phase [12] and at the expense of rapid deterioration of the analytical column. Limit of quantification of 10 ng/mL has been obtained in method described by Swart et al [9] using specific polymeric Oasis TM extraction column and injection volume of 130 l. However, the corresponding recovery of acyclovir in this method in which calibration curves were linear between the ranges of 5-1200 ng/mL was less than 50%.…”

“…Several HPLC methods [6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21] have been published for determination of acyclovir in human serum using UV or fluorescence detection. Since acyclovir is a polar compound and soluble in aqueous medium and practically insoluble in most of organic solvents, protein precipitation with perchloric acid [6][7][8]12,16,17] or solid phase extraction [9][10][11]14,15,20] are applied for pre-treatment of the drug in serum samples. While the sensitivity of analysis is significantly reduced due to dilution of the samples after deproteinization, injection of the acid supernatant after precipitation of proteins by perchloric acid leads to numerous late-eluting peaks and significant reduction of the lifetime of analytical column.…”

Development and Validation of LC-MS/MS Method for the Analysis of P-Toluenesulfonicacid, In Antiviral Drug Acyclovir

“…This liquid chromatography method was developed and validated for use in bioavailability and bioequivalence studies. Some of the analytical methods have been reported for acyclovir such as luorescence [18][19][20] direct UV [21][22][23][24] and HPLC-MS [25]. In that numerous HPLC methods have been reported for estimation of acyclovir in pharmaceutical formulations has been reported [26][27][28][29][30][31][32][33][34].…”