New Host Plants of Erwinia amylovora in Bulgaria

Atanasova, I; Hristova, Petya; Bogatzevska, Nevena; Moncheva, Penka

doi:10.1515/znc-2005-11-1212

Cited by 18 publications

(15 citation statements)

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“…the coding region for 23S rRnA is highly conservative as well as the region for 16S rRnA, which can explain the presence of an amplification product both for B. cepacia and B. pyrrocinia with the used primers. Similar results were obtained with E. amylovora and Erwinia pyrifoliae by Atanasova et al (2,3) after amplification with species-specific primers for the ams region of E. amylovora.…”

Section: Molecular Identificationsupporting

confidence: 86%

PhytopathogenicBurkholderiaSpecies in Bulb Plants in Bulgaria

Stoyanova

Kizheva

Chipeva

et al. 2011

Biotechnology & Biotechnological Equipment

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Section: Molecular Identificationsupporting

confidence: 86%

PhytopathogenicBurkholderiaSpecies in Bulb Plants in Bulgaria

Stoyanova

Kizheva

Chipeva

et al. 2011

Biotechnology & Biotechnological Equipment

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“…In 1962, Xanthomonas fragariae, the causal agent of bacterial angular leaf spot of strawberry in Minnesota, USA, was reported (Kennedy & King, 1962). Atanasova et al, 2005 described finding E. amylovora on infected plants of Fragaria ananassa and Fragaria moshata. Recently, Erwinia pyrifoliae was described as the causative agent of a bacterial disease affecting production of strawberry under greenhouse cultivation conditions.…”

Section: Introductionmentioning

confidence: 99%

The Perfect Match: Simultaneous Strawberry Pollination and Bio-Sampling of the Plant Pathogenic Bacterium Erwinia pyrifoliae by Honey Bees Apis mellifera

Steen¹,

Bergsma-Vlami²,

Wenneker³

2017

SAR

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In this study we show that honey bee colonies placed in a greenhouse for pollination of strawberry can simultaneously be used to indicate the presence of the plant pathogenic bacterium Erwinia pyrifoliae. This was demonstrated by using two methods of qualitative sacrificial and non-sacrificial bio sampling of the honey bee colony. A novel method for non-sacrificial subsampling, named the Beehold device, was applied. Applying the Beehold device did not disturb or affect negatively the honey bee colony. The study demonstrated that the integration of pollination service and bio-sampling functioned. In the sacrificially derived honey bee subsamples, E. pyrifoliae was detected prior to any visible infection in the plant; however, E. pyrifoliae was detected via non-sacrificial sampling at the same time as plant infection was first observed. The Beehold device is a practical tool for monitoring plant pathogens via forager bees during flowering until fruit onset, but is not as sensitive as directly sampling honey bees.

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“…It includes strains isolated from nine host plants and from different locations in the country. Several articles have characterised E. amylovora strains from some European countries either phenotypically, or genotypically, but only a few papers have described the distribution and initial characterisation of the pathogen in Bulgaria (Garbeva et al 1996;Bobev et al 1999;Atanasova et al 2005). Except for the study of Atanasova et al (2007), there are no papers on the diversity of Bulgarian strains of E. amylovora.…”

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confidence: 99%

“…The objective of this study was to differentiate 52 strains of E. amylovora (50 of which are Bulgarian isolates, Table 1), previously identified and phenotypically characterised by Atanasova et al (2005Atanasova et al ( , 2007, by RFLP analysis of the PstI fragment of the plasmid pEA29.…”

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confidence: 99%

“…PCR amplification of a specific 0.9-1.1 kb PstI fragment of plasmid pEA29 for all strains was carried out with the primer pairs pEA29 A and pEA29 B (Bereswill et al 1992) using the procedure previously described by Atanasova et al (2005). RFLP analysis of the amplified fragment obtained by the primers mentioned above was carried out to investigate the genetic differentiation among the Bulgarian E. amylovora strains.…”

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Differentiation of Erwinia amylovora strains from Bulgaria by PCR-RFLP analysis

et al. 2009

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Fifty strains of Erwinia amylovora isolated in Bulgaria from different host plants and locations as well as in different years were analysed by RFLP analysis of the pEA29 PstI amplified fragment with HpaII. All the strains formed three well-resolved fragments (large-from 365 to 440 bp, mediumabout 341 bp and small-about 180 bp).The strains were classified into three RFLP groups based on the polymorphism in the length of the largest fragment. This fragment was of intermediate size for 63% of the strains, and it was the longest (from 410 to 440 bp) for 29% of the strains. The variable region was sequenced for five strains. The DNA sequence analysis confirmed the different size of the largest fragment. Ten or more than ten SSRs were found for the strains in the group with the largest size of the largest fragment. Some correlation between the RFLP profiles and the origin of the strains was revealed. The RFLP profiles displayed stability in certain strains isolated from the same trees and orchards, but in different years. The number of SSRs was different in strains isolated from one and the same host plant, orchard and year, and also in strains isolated from the same host plant and orchard, but in different years. This could indicate that under natural conditions the fire blight symptoms might be caused by a mixture of E. amylovora strains with different SSR numbers, and so coexistence of distinguishable strains or a change in the population could be assumed.

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New Host Plants of Erwinia amylovora in Bulgaria

Cited by 18 publications

References 0 publications

PhytopathogenicBurkholderiaSpecies in Bulb Plants in Bulgaria

PhytopathogenicBurkholderiaSpecies in Bulb Plants in Bulgaria

The Perfect Match: Simultaneous Strawberry Pollination and Bio-Sampling of the Plant Pathogenic Bacterium Erwinia pyrifoliae by Honey Bees Apis mellifera

Differentiation of Erwinia amylovora strains from Bulgaria by PCR-RFLP analysis

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