The adiponectin promoter polymorphism rs266729 is associated with gestational diabetes. The minor G allele appears to confer protection against pregnancy-related diabetes mellitus. This effect is probably due to the influence of the variant on the adiponectin transcription regulation during gestation.

Nine strains of Erwinia amylovora were isolated from new host plants in Bulgaria D chokeberry and strawberry. The strains were characterized morphologically and biochemically using the API 20E and BIOLOG system. It was established that they showed three different API 20E metabolic profiles, not found by previous studies of E. amylovora. All strains were identified as E. amylovora due to their metabolic fingerprint patterns obtained by the BIOLOG system. The identification was confirmed by PCR amplification of a specific region of plasmid pEA29 and genome ams-region. This study is the first characterization and identification of E. amylovora strains isolated from chokeberry and strawberry by the API 20E and BIOLOG system and by polymerase chain reaction.

Esta obra, editada por José María Infante Bonfiglio y María Eugenia Flores Treviño, recoge doce trabajos de investigación presentados en el VI Coloquio Internacional del Programa EDICE (Estudios sobre el Discurso de la Cortesía en Español), celebrado en 2011 en Monterrey (México). Dichos trabajos aparecen organizados en tres partes, dedicadas respectivamente a estudios sobre imagen y (des)cortesía, actos de habla y (des)cortesía y atenuación.Los autores cuyas investigaciones integran este libro asumen que la (des) cortesía varía de una cultura a otra, y, por tanto, se basan en postulados teóricos de pragmática sociocultural. En este sentido, adquieren gran importancia las nociones de Bravo (otros trabajos), quien enriqueció las categorías analíticas básicas antecedentes de carácter universalista, dando cuenta de la importancia de los factores de variación sociocultural en los estudios de (des)cortesía.Al inicio, los editores ofrecen una reflexión sobre ciertas deficiencias tanto formales como conceptuales prototípicas en estudios de esta índole, tales como la falta de consenso entre los investigadores en cuanto a la metodología y la terminología empleadas. Se defiende la necesidad de contemplar la concepción que se tiene de determinados valores culturales a la hora de abordar los estudios sobre (des)cortesía. Asimismo, critican el hecho de que la mayoría de los estudiosos se aferren excesivamente a las teorías de autores precedentes para avalar sus investigaciones. Esta crítica se centra en aquellos trabajos fundamentados en los postulados universalistas de Brown y Levinson (1987), los cuales no pueden sostenerse, según los autores, si se examinan los avances de la teoría social de las últimas décadas (p. 11).La primera parte de la obra se abre con un trabajo de Carmen Curcó Cobos, en el que se defiende la postura contraria. Esta autora aboga por el conservadurismo metodológico. Sin dejar de otorgar importancia a la consideración de valores culturales, apuesta por no abandonar la clásica noción de imagen pública. Seguidamente, hace un recorrido bibliográfico por trabajos de autores -9786072703285

The efficiencies of two dosage schedules of amikacin (2 x 10 mg/kg of body weight per 24 h and 1 X 20 mg/kg/24 h intramuscularly for 5 days) against Pseudomonas aeruginosa sepsis in rabbits were compared. Blood samples were drawn at various times after the first application, and amikacin concentrations in serum were assayed microbiologically. The dynamics of the bactericidal effect of amikacin was simulated in vitro with the same strain of P. aeruginosa. No regrowth was found with the 20-mg/kg dose when the bacterial inoculum was in contact with experimental and theoretically predicted serum amikacin concentrations. The killing effect was present even when the drug levels decreased considerably below the MIC. The interrelationship between simulated amikacin concentrations in serum and the corresponding average killing rates was described appropriately by the standard Emax model. The higher amikacin dose performed its bactericidal effect faster and the drug persisted longer in the blood. The two amikacin regimens were therapeutically equivalent, but the once-daily schedule had some advantages over the twice-daily drug administration which became evident when both the pharmacokinetic and the pharmacodynamic parameters of the drug were considered.Aminoglycoside antibiotics play a major role in the therapy of severe gram-negative infections. Pseudomonas aeruginosa sepsis continues to have a high mortality rate (26), so therapeutic strategies to overcome this problem were investigated. Aminoglycoside antibiotics are potent antipseudomonal agents having rapid and concentration-dependent bactericidal activity, but because of their narrow safety margins new approaches are necessary in order to optimize their therapeutic efficacy. One such approach is reducing the frequency of aminoglycoside administration (25). The pharmacodynamic background for once-daily dosing of amikacin includes concentrationdependent bactericidal activity (24), postantibiotic effect (5, 6), and low incidence of resistant subpopulations when concentrations in plasma above the threshold (8 to 10 times the MIC) are achieved (1, 21). An optimal regimen for aminoglycoside therapy would assure an adequate initial drug level, thus minimizing the opportunity for adaptive resistance, reducing the need for drug monitoring, and avoiding severe drug toxicity (18). A wide variety of animal models has been investigated, and the findings suggest that the less frequent dosing does not alter drug effectiveness (27,34). The efficiencies of two dosage schedules of amikacin (2 x 10 mg/kg of body weight per 24 h and 1 x 20 mg/kg/24 h given intramuscularly) against experimental P. aeruginosa sepsis in rabbits were compared. An attempt to correlate pharmacokinetic and pharmacodynamic features of the drug simulating its bactericidal in vitro activity was made.MATERIALS AND METHODS Animals. Chinchilla rabbits divided into three groups (six animals each) with a mean body weight of 3.12 ± 0.36 kg were included in the study.Pseudomonas infection model. Sepsis was induced by ...

Aim Improvement and harmonization of European clinical pharmacology and therapeutics (CPT) education is urgently required. Because digital educational resources can be easily shared, adapted to local situations and re‐used widely across a variety of educational systems, they may be ideally suited for this purpose. Methods With a cross‐sectional survey among principal CPT teachers in 279 out of 304 European medical schools, an overview and classification of digital resources was compiled. Results Teachers from 95 (34%) medical schools in 26 of 28 EU countries responded, 66 (70%) of whom used digital educational resources in their CPT curriculum. A total of 89 of such resources were described in detail, including e‐learning (24%), simulators to teach pharmacokinetics and/or pharmacodynamics (10%), virtual patients (8%), and serious games (5%). Together, these resources covered 235 knowledge‐based learning objectives, 88 skills, and 13 attitudes. Only one third (27) of the resources were in‐part or totally free and only two were licensed open educational resources (free to use, distribute and adapt). A narrative overview of the largest, free and most novel resources is given. Conclusion Digital educational resources, ranging from e‐learning to virtual patients and games, are widely used for CPT education in EU medical schools. Learning objectives are based largely on knowledge rather than skills or attitudes. This may be improved by including more real‐life clinical case scenarios. Moreover, the majority of resources are neither free nor open. Therefore, with a view to harmonizing international CPT education, more needs to be learned about why CPT teachers are not currently sharing their educational materials.