New hyper-variable SSRs for diversity analysis in mango (Mangifera indica L.)

Srivastav, Manish; Singh, Sanjay K.; Prakash, Jai; Singh, Rakesh; Sharma, Neha; Ramchandra, Sridhar; Devi, Ruma; Gupta, Ankit; Mahto, A.K.; Jayaswal, Pawan Kumar; Singh, S. P.; Singh, Nagendra

doi:10.31742/ijgpb.81.1.13

Cited by 11 publications

(8 citation statements)

References 24 publications

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“…SSR markers have been widely used to differentiate mango cultivars and identify genetic diversity [11,20], distinguish some synonymous (identical SSR genotypes with different cultivar names) and homonymous (different SSR genotypes with the same cultivar name) accessions [24], genetic relatedness [10] and so on. All these SSR markers will be used in the future studies of genetic diversity, genetic map construction and marker-assisted selection and utilized for breeding programs in mango.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, SSR uorescent labeling technology has been developed and successfully applied to variety identi cation of grape [13], bayberry [14], watermelon [15] and other crops [16,17] due to its high e ciency, accuracy, sensitivity and automation. At present, only a few literatures have reported the application of SSR uorescent labeling technology to identify the authenticity of mango hybrids [18], genetic diversity and genetic relatedness of cultivars [19][20][21], con rmation of maternal or zygotic origin of embryogenic cultures [22]. In this study, F 1 hybrid population of 'Jinhuang' and 'Renong No.1' were used as experimental materials.…”

Section: Introductionmentioning

confidence: 99%

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Identification of F1 hybrid progenies in mango based on Fluorescent SSR markers

Zheng

et al. 2022

Preprint

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Background Artificial cross breeding (hand pollination) is an important method for breeding new mango cultivars. It is easy to produce false hybrids during hybridization, which makes it difficult to distinguish the authenticity of hybrid offspring. Therefore, it is necessary to identify the authenticity of hybrids. Results Mango ‘Jinhuang’ and ‘Renong No.1’ and 65 individual plants of their F1 hybrids were used as experimental materials, eight SSR primer pairs with polymorphism in parents were used to identify the F1 hybrids by capillary electrophoresis. The results showed that a total of 62 true hybrids were identified from 65 hybrid progenies, and the true hybrid rate was 95.38%. According to the segregation type of co-dominant SSR markers in F1 generation, the segregation ratio of different alleles at the same locus of eight markers was performed by x2 test. Results showed that eight SSR loci were consistent with Mendel’s segregation law. The results of cluster analysis showed that the parents and 62 true hybrids could be classified into two categories at 0.58: the first category contained 27 offspring, clustered with ‘Jinhuang’ and showed a maternal genetic tendency. The second category contained 35 offspring, clustered with ‘Renong No.1’ and showed a partial paternal genetic tendency. DNA fingerprint of hybrids from ‘Jinhuang’ × ‘Renong No.1’ cross were constructed using eight SSR primers for variety protection. Conclusion We used eight SSR fluorescent markers to test the authenticity of hybrids derived from crossing combinations of ‘Jinhuang’ and ‘Renong No.1’. A total of 62 true hybrids were identified from 65 hybrid progenies, which would lay a foundation for the follow-up mango cross breeding and molecular marker assisted breeding. In addition, the development of hybrid fingerprints could facilitate variety protection.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of F1 hybrid progenies in mango based on Fluorescent SSR markers

Zheng

et al. 2022

Preprint

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“…146 Srivastav et al demonstrated that 110 hyper-variable SSR markers were useful in discriminating mango germplasm and further proposed that these SSRs can serve for mapping of QTLs in mangoes. 168 Recent studies on the whole genome of Philippine mango species revealed the genome-wide variants (SNPs and InDels) including putative genes involved in mango fruit development, disease resistance, and economically important traits. 169 ■ CONCLUSIONS AND FUTURE PROSPECTIVE Improvement in fruit nutritional content and maintaining postharvest storage quality are key aspects of mango production.…”

Section: ■ Proteome Analysis Of Mango Fruitsmentioning

confidence: 99%

“…A set of genetic markers including with precise analysis methods will become valuable materials for mango breeders, which leads to the identification of regions in the genome that are associated with economically important traits beneficial for marker assisted selection in mangoes . Srivastav et al demonstrated that 110 hyper-variable SSR markers were useful in discriminating mango germplasm and further proposed that these SSRs can serve for mapping of QTLs in mangoes . Recent studies on the whole genome of Philippine mango species revealed the genome-wide variants (SNPs and InDels) including putative genes involved in mango fruit development, disease resistance, and economically important traits …”

Section: Molecular Genetic Approachesmentioning

confidence: 99%

Advances in Physiological, Transcriptomic, Proteomic, Metabolomic, and Molecular Genetic Approaches for Enhancing Mango Fruit Quality

Datir

Regan

2022

J. Agric. Food Chem.

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Mango (Mangifera indica L.) is a nutritionally important fruit of high nutritive value, delicious in taste with an attractive aroma. Due to their antioxidant and therapeutic potential, mango fruits are receiving special attention in biochemical and pharmacognosy-based studies. Fruit quality determines consumer’s acceptance, and hence, understanding the physiological, biochemical, and molecular basis of fruit development, maturity, ripening, and storage is essential. Transcriptomic, metabolomic, proteomic, and molecular genetic approaches have led to the identification of key genes, metabolites, protein candidates, and quantitative trait loci that are associated with enhanced mango fruit quality. The major pathways that determine the fruit quality include amino acid metabolism, plant hormone signaling, carbohydrate metabolism and transport, cell wall biosynthesis and degradation, flavonoid and anthocyanin biosynthesis, and carotenoid metabolism. Expression of the polygalacturonase, cutin synthase, pectin methyl esterase, pectate lyase, β-galactosidase, and ethylene biosynthesis enzymes are related to mango fruit ripening, flavor, firmness, softening, and other quality processes, while genes involved in the MAPK signaling pathway, heat shock proteins, hormone signaling, and phenylpropanoid biosynthesis are associated with diseases. Metabolomics identified volatiles, organic acids, amino acids, and various other compounds that determine the characteristic flavor and aroma of the mango fruit. Molecular markers differentiate the mango cultivars based on their geographical origins. Genetic linkage maps and quantitative trait loci studies identified regions in the genome that are associated with economically important traits. The review summarizes the applications of omics techniques and their potential applications toward understanding mango fruit physiology and their usefulness in future mango breeding.

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“…Diversity studies will allow a wider understanding on the genome accessions with variable fruit characters and their origins. As the sequencing technologies improved, the generation of genomic knowledge of fruit crops progressed from application of molecular markers for genetic diversity analysis [ 214 , 215 ] and DNA fingerprinting [ 216 ] to the generation of high throughput sequencing based SNPs, TRAPs (Target Region Amplification Polymorphism) and SSR markers [ 217 , 218 ]. SNP genotyping [ 219 ] was developed based on transcriptome and whole genome sequence information in mango and banana.…”

Section: Genomic Assisted Breeding Strategies In Tropical Fruit Cropsmentioning

confidence: 99%

Genomic Approaches for Improvement of Tropical Fruits: Fruit Quality, Shelf Life and Nutrient Content

Mathiazhagan

Chidambara

Laxman

et al. 2021

Genes

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The breeding of tropical fruit trees for improving fruit traits is complicated, due to the long juvenile phase, generation cycle, parthenocarpy, polyploidy, polyembryony, heterozygosity and biotic and abiotic factors, as well as a lack of good genomic resources. Many molecular techniques have recently evolved to assist and hasten conventional breeding efforts. Molecular markers linked to fruit development and fruit quality traits such as fruit shape, size, texture, aroma, peel and pulp colour were identified in tropical fruit crops, facilitating Marker-assisted breeding (MAB). An increase in the availability of genome sequences of tropical fruits further aided in the discovery of SNP variants/Indels, QTLs and genes that can ascertain the genetic determinants of fruit characters. Through multi-omics approaches such as genomics, transcriptomics, metabolomics and proteomics, the identification and quantification of transcripts, including non-coding RNAs, involved in sugar metabolism, fruit development and ripening, shelf life, and the biotic and abiotic stress that impacts fruit quality were made possible. Utilizing genomic assisted breeding methods such as genome wide association (GWAS), genomic selection (GS) and genetic modifications using CRISPR/Cas9 and transgenics has paved the way to studying gene function and developing cultivars with desirable fruit traits by overcoming long breeding cycles. Such comprehensive multi-omics approaches related to fruit characters in tropical fruits and their applications in breeding strategies and crop improvement are reviewed, discussed and presented here.

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New hyper-variable SSRs for diversity analysis in mango (Mangifera indica L.)

Cited by 11 publications

References 24 publications

Identification of F1 hybrid progenies in mango based on Fluorescent SSR markers

Identification of F1 hybrid progenies in mango based on Fluorescent SSR markers

Advances in Physiological, Transcriptomic, Proteomic, Metabolomic, and Molecular Genetic Approaches for Enhancing Mango Fruit Quality

Genomic Approaches for Improvement of Tropical Fruits: Fruit Quality, Shelf Life and Nutrient Content

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