New <i>Lactococcus</i> Strain with Immunomodulatory Activity: Enhancement of Th1‐Type Immune Response

Kimoto, Hiromi; Mizumachi, Koko; Okamoto, Takashi; Kurisaki, Jun Ichi

doi:10.1111/j.1348-0421.2004.tb03490.x

Cited by 99 publications

(80 citation statements)

References 24 publications

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“…Probiotic bacteria of Lactococcus lactis subsp. lactis G50 was stated to increase cytokines related with Th1 and showed anti-inflammatory effect by suppressing the hypersensitivity reactions (Kimoto et al 2004). We found that IL-6 levels were significantly decreased in the probiotic, exercise, and restriction subgroups of both young and old groups.…”

Section: Discussionmentioning

confidence: 66%

In case of obesity, longevity-related mechanisms lead to anti-inflammation

Kaya

Bayiroğlu

Mıs

et al. 2013

AGE

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The exact mechanisms which contribute to longevity have not been figured out yet. Our aim was to find out a common way for prompting longevity by bringing together the well-known applications such as food restriction, exercise, and probiotic supplementing in an experimental obesity model. Experimental obesity was promoted in a total of 32 young (2 months old) and 32 aged (16 months old) male Wistar albino rats through 8-week cafeteria diet (salami, chocolate, chips, and biscuits). Old and young animals were divided into groups each consisting of eight animals and also divided into four subgroups as obese control, obese food restriction, obese probiotic-fed and obese exercise groups. Probiotic group diet contained 0.05 %w/total diet inactive and lyophilized Lactobacillus casei str. Shirota. The exercise group was subjected to treadmill running 1 h/day, at 21 m/min and at an uphill incline of 15 % for 5 days a week. Food restriction group was formed by giving 40 % less food than the others. The control group was fed regular pellet feed ad libitum. This program was continued for 16 weeks. Blood samples from all the groups were analyzed for fasting glucose, insulin, IGF-1, insulin-like growth factor binding protein 3 (IGFBP-3), interleukin (IL)-6, IL-12, malondialdehyde (MDA), fT3, TT3, fT4, TT4, and liver tissue MDA levels were measured. All applications showed anti-inflammatory effects through the observed changes in the levels of IGFBP-3, IL-6, and IL-12 in the young and old obese rats. While the interventions normally contribute to longevity by recruiting different action mechanisms, anti-inflammatory effect is the only mode of action for all the applications in the obesity model.

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Section: Discussionmentioning

confidence: 66%

In case of obesity, longevity-related mechanisms lead to anti-inflammation

Kaya

Bayiroğlu

Mıs

et al. 2013

AGE

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“…Lactis G50 killed by heat to induce cytokine production of macrophages in culture, was previously proved by Kimoto et al (2004), it was observed that this strain continued to induce cytokine production, suggesting that such activity is associated with elements on the bacterial wall. Ghadimi et al (2008) proved that Lactobacillus rhamnosus GG, Lactobacillus gasseri (PA16/8), Bifidobacterium bifidum (MP20/5) and Bifidobacterium longum (SP07/3), as well as their genomic DNA, dosedependently modulated the TH1/TH2 response to allergens.…”

Section: Discussionmentioning

confidence: 71%

The Immunomodulatory Effects of Probiotic Bacteria on Peripheral Blood Mononuclear Cells (Pbmcs) of Allergic Patients

Sheikh¹,

Shalaby²,

Hafez³

et al. 2014

American Journal of Immunology

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Allergic diseases represent major health burden. An allergic reaction is characterized by a disrupted Thelper 1⁄T-helper 2 balance toward a preferential allergen specifically induced TH2 cytokine profile, causing allergic inflammation Probiotic bacteria have various benificial effects in many pathologic situation. Studies have shown that the bacteria present in the intestinal micro flora play a role in the TH1/TH2 balance and its modulation can promote the control of infectious and immune processes. Testing the effects of probiotic bacteria on TH1/TH2 cytokine production by peripheral blood mononuclear cells of allergic patients and control subjects. This study included 24 patients allergic to date pollen and 16 healthy control subjects. PBMC of both groups were separated and cultured for 72 h with date pollen allergen (home-made) in the presence or absence of Lactobacillus rhamnosus ATCC 7469 (Living and dead) and Cphycocyanin (extracted from Spirulina platensis). The cell culture supernatants were collected to measure Interlukin 4 and Interferon gamma by quantitative ELISA. Incubation of PBMCs of allergic patients with living Lactobacillus rhamnosus ATCC 7469 showed marked reduction in IL4 production (median IL4 concentarion = 3.9 pg.) compared to PBMCs callenged with pollen alone (mediam IL4 conentration = 52.6 pg). When PBMC were incubated with living Lactobacillus rhamnosus in absence of allergen significant increase in and IFNγ (median concentration = 42.75 pg.) was obtained, compared to PBMC challenged with allergen alone (median = 22.8 pg). When PBMCs incubated with heat killed Lactobacillus rhamnosus either in presence or absence of the offending allergen, marked reduction in IL4 production was obtained (median = 10.6, 3.6 pg respectively) compared to PBMC incubated with allergen alone (median = 52.6 pg). When PBMCs incubated with dead Lactobacillus rhamnosus, marked increase in IFNγ production was obtained (median = 49 pg) when compared to IFNγ production by PBMC challenged with allergen (median 22.8 pg). PBMCs challenged with PC in the presence or absence of allergen showed marked decrease of IL4 production (median = 19.8, 17 pg respectively) when compared to PBMC incubated with the offending allergen alone (median = 52.6 pg). PBMCs incubated with PC showed significant increase of and IFNγ production (median= 319.6 pg) when compared to PBMC incubated with the offending allergen alone (median = 22.8 pg). Conclusion Lactobacillus rhamnosus ATCC 7469 and C-phycocyanin (extracted from Spirulina platensis) inversed the TH1: TH2 polarization in allergic patients and could be a promissing line of treatmen.

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“…Lactococcus strain G50 described above did not produce slime products. Induction of cytokine production in clonal macrophage by the heatkilled cells of strain G50 was observed 19 , indicating that it is not critical whether cells are viable or nonviable (heatkilled) for the immunomodulatory effects of strain G50 although strain G50 was proved to reach the intestine alive by using mice (unpublished data). In strain G50, cell wall components, which constitute heat-killed cells, may be a factor for immune modulation by this strain.…”

Section: Immune Modulationmentioning

confidence: 99%

Lactococcus sp. as Potential Probiotic Lactic Acid Bacteria

Kimoto‐Nira

Mizumachi

Nomura

et al. 2007

JARQ

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The most widely used probiotic bacteria are lactobacilli and bifidobacteria, which have been isolated from the human gastrointestinal tract. The development of new probiotic strains, which are more feasible and beneficial organisms, is awaited in the dairy industry. Lactococci would be promising species because of their extensive usage in manufacturing dairy products such as cheese and fermented milk. However, there have been few studies on the probiotic activity of lactococci since they are traditionally not considered to be natural inhabitants of the human gastrointestinal tract. Recently, several works showed the possibility of the presence of lactococci in the flora of the human or animal gastrointestinal tract. In this review, we would like to propose Lactococcus sp. as new probiotic bacteria.

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New Lactococcus Strain with Immunomodulatory Activity: Enhancement of Th1‐Type Immune Response

Cited by 99 publications

References 24 publications

In case of obesity, longevity-related mechanisms lead to anti-inflammation

In case of obesity, longevity-related mechanisms lead to anti-inflammation

The Immunomodulatory Effects of Probiotic Bacteria on Peripheral Blood Mononuclear Cells (Pbmcs) of Allergic Patients

Lactococcus sp. as Potential Probiotic Lactic Acid Bacteria

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